HAMAP rule MF_02003
General rule information
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| Accession | MF_02003 |
| Dates | 5-DEC-2005 (Created) 19-NOV-2022 (Last updated, Version 24) |
| Name | Ile_tRNA_synth_type2 |
| Scope | Bacteria
Archaea |
| Templates | P56690 (SYI_THET8); P00956 (SYI_ECOLI); P41368 (SYI2_STAAU); P9WFV3 (SYI_MYCTU); Q8L1B1 (SYI2_PSEFL): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). |
| Catalytic activity | RHEA:11060: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)
EC 6.1.1.5 |
| Cofactor | Zn(2+) |
| Subunit | Monomer. |
| Subcellular location | Cytoplasm. |
| Domain | IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)). |
| Similarity | Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. |
Keywords
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Cytoplasm
Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis
Metal-binding
Zinc
Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis
Metal-binding
Zinc
Gene Ontology
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GO:0004822; Molecular function: isoleucine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0006428; Biological process: isoleucyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0005524; Molecular function: ATP binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0006428; Biological process: isoleucyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
| Pfam | PF00133; tRNA-synt_1; 1; |
| PRINTS | PR00984; TRNASYNTHILE; 1; |
| TIGRFAMs | TIGR00392; IleS; 1; |
Features
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| From: SYI_THET8 (P56690) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 47 | 57 | 'HIGH' region | |||||||||
| MOTIF | 591 | 595 | 'KMSKS' region | |||||||||
| BINDING | 594 | 594 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | K | ||||||||
Additional information
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| Size range | 981-1213 amino acids |
| Related rules | MF_02002 (SYI) |
| Fusion | None |
| Comments | Some species have a second copy of IleRS in another subfamily (MF_02002). MYCTU, PSEFL and STAAU confer high-level resistance to the antibiotic mupirocin (pseudomonic acid A), an Ile-analog that competitively inhibits activation by Ile-tRNA synthetase, thus inhibiting protein biosynthesis. Shorter N-terminal in SACS2; sequence not included in alignment and not taken into account in size range. |