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Annotation rule MF_02004
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General rule information [?]

Accession MF_02004
Dates 7-FEB-2006 (Created)
20-NOV-2019 (Last updated, Version 13)
Name Val_tRNA_synth_type1
Scope
Bacteria
Templates P07118 (SYV_ECOLI); P96142 (SYV_THETH); P11931 (SYV_GEOSE): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYV
Protein name
RecName: Full=Valine--tRNA ligase;
EC 6.1.1.9;
AltName: Full=Valyl-tRNA synthetase;
Short=ValRS;
Gene name
valS

Comments [?]

Function Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.
Catalytic activity RHEA:10704: ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)
EC 6.1.1.9
Subunit Monomer.
Subcellular location Cytoplasm.
Domain ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.
The C-terminal coiled-coil domain is crucial for aminoacylation activity.
Similarity Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004832; Molecular function: valine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0006438; Biological process: valyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS00178; AA_TRNA_LIGASE_I; 1;
Pfam PF00133; tRNA-synt_1; 1;
PF08264; Anticodon_1; 1;
PRINTS PR00986; TRNASYNTHVAL; 1;
TIGRFAMs TIGR00422; ValS; 1;

Computed features [?]

General Coiled_coil; -; 1-unlimited; trigger=yes;

Features [?]

From: SYV_ECOLI (P07118)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     42     52       'HIGH' region     P-N-[VIL]-T-G-x-[LI]-H-[MLIV]-G-H  
MOTIF     554     558       'KMSKS' region     K-[MLF]-[ST]-K-[ST]  
BINDING     557     557       ATP     K  

Additional information [?]

Size range 822-1020 amino acids
Related rules MF_02005 (SYV)
Fusion None