HAMAP rule MF_02004
General rule information
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| Accession | MF_02004 |
| Dates | 7-FEB-2006 (Created) 1-JUN-2023 (Last updated, Version 16) |
| Name | Val_tRNA_synth_type1 |
| Scope | Bacteria |
| Templates | P07118 (SYV_ECOLI); P96142 (SYV_THETH); P11931 (SYV_GEOSE): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner. |
| Catalytic activity | RHEA:10704: ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)
EC 6.1.1.9 |
| Subunit | Monomer. |
| Subcellular location | Cytoplasm. |
| Domain | ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site. |
| The C-terminal coiled-coil domain is crucial for aminoacylation activity. | |
| Similarity | Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. |
Keywords
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Cytoplasm
Aminoacyl-tRNA synthetase
ATP-binding
Coiled coil
Ligase
Nucleotide-binding
Protein biosynthesis
Aminoacyl-tRNA synthetase
ATP-binding
Coiled coil
Ligase
Nucleotide-binding
Protein biosynthesis
Gene Ontology
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GO:0004832; Molecular function: valine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0006438; Biological process: valyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0005524; Molecular function: ATP binding.
GO:0006438; Biological process: valyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
| Pfam | PF00133; tRNA-synt_1; 1; |
| PF08264; Anticodon_1; 1; | |
| PRINTS | PR00986; TRNASYNTHVAL; 1; |
| NCBIfam | TIGR00422; ValS; 1; |
Computed features
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| General | Coiled_coil; -; 1-unlimited; trigger=yes; |
Features
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| From: SYV_ECOLI (P07118) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 42 | 52 | 'HIGH' region | P-N-[VIL]-T-G-x-[LI]-H-[MLIV]-G-H | ||||||||
| MOTIF | 554 | 558 | 'KMSKS' region | K-[MLF]-[ST]-K-[ST] | ||||||||
| BINDING | 557 | 557 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | K | ||||||||
Additional information
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| Size range | 822-1020 amino acids |
| Related rules | MF_02005 (SYV) |
| Fusion | None |