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HAMAP rule MF_02004

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General rule information [?]

Accession MF_02004
Dates 7-FEB-2006 (Created)
1-JUN-2023 (Last updated, Version 16)
Name Val_tRNA_synth_type1
Scope(s) Bacteria
Template(s) P07118 (SYV_ECOLI); P96142 (SYV_THETH); P11931 (SYV_GEOSE); [ Recover all ]
Triggered by HAMAP; MF_02004 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYV
Protein name RecName: Full=Valine--tRNA ligase;
AltName: Full=Valyl-tRNA synthetase;
Gene name Name=valS;

Comments [?]

FUNCTIONCatalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner.
CATALYTIC ACTIVITY Reaction=ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl- tRNA(Val); Xref=Rhea:RHEA:10704, Rhea:RHEA-COMP:9672, Rhea:RHEA- COMP:9708, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57762, ChEBI:CHEBI:78442, ChEBI:CHEBI:78537, ChEBI:CHEBI:456215; EC=;
DOMAINValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.
DOMAINThe C-terminal coiled-coil domain is crucial for aminoacylation activity.
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004832; Molecular function:valine-tRNA ligase activity
GO:0005524; Molecular function:ATP binding
GO:0006438; Biological process:valyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00133; tRNA-synt_1; 1;
Pfam PF08264; Anticodon_1; 1;
NCBIfam TIGR00422; ValS; 1;
General Coiled_coil; -; 1-unlimited;

Features [?]

From: SYV_ECOLI (P07118)
Key From To Description Tag Condition FTGroup
MOTIF 42 52 /note="'HIGH' region" P-N-[VIL]-T-G-x-[LI]-H-[MLIV]-G-H
MOTIF 554 558 /note="'KMSKS' region" K-[MLF]-[ST]-K-[ST]
BINDING 557 557 /ligand="ATP"

Additional information [?]

Size range 822-1020 amino acids
Related rules MF_02005
Fusion Nter: None Cter: None

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