HAMAP rule MF_02038
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_02038 |
| Accession | MF_02038 |
| Dates | 01-JUL-2015 (Created)
17-JUN-2025 (Last updated, Version 8) |
| Name | EgtE |
| Scope(s) |
Bacteria Actinomycetota |
| Template(s) | A0R5M7; [ Recover all ] |
| Triggered by |
HAMAP; MF_02038 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | EGTE |
| Protein name | RecName: Full=Hercynylcysteine sulfoxide lyase; EC=4.4.1.36; |
| Gene name | Name=egtE; |
Comments
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| FUNCTION | Catalyzes a C-S bond cleavage, converting hercynylcysteine sulfoxide to 2-sulfenohercynine, a sulfenic acid intermediate that can be spontaneously reduced to ergothioneine. Ergothioneine is a secreted antioxidant that protects from oxidative stress. |
| CATALYTIC ACTIVITY | Reaction=S-(hercyn-2-yl)-L-cysteine S-oxide + AH2 + H(+) = ergothioneine + pyruvate + A + NH4(+); Xref=Rhea:RHEA:42688, ChEBI:CHEBI:13193, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:28938, ChEBI:CHEBI:82706, ChEBI:CHEBI:134344; EC=4.4.1.36; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42689; |
| CATALYTIC ACTIVITY | Reaction=S-(hercyn-2-yl)-L-cysteine S-oxide + H2O = 2-sulfenohercynine + pyruvate + NH4(+); Xref=Rhea:RHEA:52780, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:82706, ChEBI:CHEBI:136834; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52781; |
| COFACTOR | Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; |
| PATHWAY | Amino-acid biosynthesis; ergothioneine biosynthesis. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. EgtE subfamily. |
Keywords
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Gene Ontology
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| GO:0016846; Molecular function:carbon-sulfur lyase activity |
| GO:0052699; Biological process:ergothioneine biosynthetic process |
Cross-references
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Features
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| From: EGTE_MYCS2 (A0R5M7) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 48 | 48 | /ligand="pyruvate" /ligand_id="ChEBI:CHEBI:15361" |
Y | ||||||||
| BINDING | 82 | 82 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" |
S | ||||||||
| BINDING | 83 | 83 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" |
N | ||||||||
| BINDING | 106 | 106 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" |
Y | ||||||||
| BINDING | 183 | 183 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" |
Q | ||||||||
| BINDING | 183 | 183 | /ligand="pyruvate" /ligand_id="ChEBI:CHEBI:15361" |
Q | ||||||||
| BINDING | 200 | 200 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" |
S | ||||||||
| BINDING | 202 | 202 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" |
R | ||||||||
| BINDING | 337 | 337 | /ligand="pyruvate" /ligand_id="ChEBI:CHEBI:15361" |
R | ||||||||
| BINDING | 348 | 348 | /ligand="pyruvate" /ligand_id="ChEBI:CHEBI:15361" |
R | ||||||||
| MOD_RES | 203 | 203 | /note="N6-(pyridoxal phosphate)lysine" | K | ||||||||
Additional information
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| Size range | 359-425 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |