HAMAP rule MF_02050

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_02050
Accession	MF_02050
Dates	06-JAN-2016 (Created) 03-SEP-2024 (Last updated, Version 8)
Name	IcmF
Scope(s)	Bacteria
Template(s)	Q1LRY0; Q5Z110; Q5KUG0; Q146L7; [ Recover all ]
Triggered by	HAMAP; MF_02050 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	ICMF
Protein name	RecName: Full=Fused isobutyryl-CoA mutase; Includes: RecName: Full=Isobutyryl-CoA mutase; Short=ICM; EC=5.4.99.13; Includes: RecName: Full=P-loop GTPase; EC=3.6.5.-; AltName: Full=G-protein chaperone;
Gene name	Name=icmF;

Comments [?]

FUNCTION	Catalyzes the reversible interconversion of isobutyryl-CoA and n-butyryl-CoA, using radical chemistry. Also exhibits GTPase activity, associated with its G-protein domain (MeaI) that functions as a chaperone that assists cofactor delivery and proper holo-enzyme assembly.
CATALYTIC ACTIVITY	Reaction=2-methylpropanoyl-CoA = butanoyl-CoA; Xref=Rhea:RHEA:13141, ChEBI:CHEBI:57338, ChEBI:CHEBI:57371; EC=5.4.99.13;
CATALYTIC ACTIVITY	Reaction=GTP + H2O = GDP + phosphate + H(+); Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189;
COFACTOR	Name=adenosylcob(III)alamin; Xref=ChEBI:CHEBI:18408;
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SUBUNIT	Homodimer.
DOMAIN	Is composed of four functional domains: the N-terminal 5'- deoxyadenosylcobalamin binding region that is homologous to the small subunit of ICM (IcmB), a middle P-loop GTPase domain (MeaI) that likely acts as a chaperone for ICM, a structured linker region involved in dimer formation, and a C-terminal part that is homologous to the large substrate-binding subunit of ICM (IcmA).
SIMILARITY	Belongs to the IcmF family.

Keywords [?]

Multifunctional enzyme

Nucleotide-binding

Gene Ontology [?]

GO:0031419; Molecular function:cobalamin binding

GO:0005525; Molecular function:GTP binding

GO:0003924; Molecular function:GTPase activity

GO:0047727; Molecular function:isobutyryl-CoA mutase activity

GO:0000287; Molecular function:magnesium ion binding

GO:0006637; Biological process:acyl-CoA metabolic process

Cross-references [?]

PROSITE	PS51332; B12_BINDING; 1;
Pfam	PF02310; B12-binding; 1;
Pfam	PF01642; MM_CoA_mutase; 1;
NCBIfam	TIGR00641; acid_CoA_mut_N; 1;
NCBIfam	TIGR00640; acid_CoA_mut_C; 1;

Features [?]

From: ICMF_CUPMC (Q1LRY0)

Key

From

Description

Tag

Condition

FTGroup

BINDING

219

224

/ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"

G-[AS]-G-K-S-[ST]

BINDING

357

360

/ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"

N-K-x-[DE]

REGION

169

417

/note="GTPase chaperone MeaI"

REGION

418

579

/note="Linker"

BINDING

/ligand="adenosylcob(III)alamin"
/ligand_id="ChEBI:CHEBI:18408"
/ligand_part="Co"
/ligand_part_id="ChEBI:CHEBI:27638"
/note="axial binding residue"

BINDING

223

/ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
/ligand_note="catalytic"

BINDING

248