HAMAP rule MF_02098
General rule information
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| Accession | MF_02098 |
| Dates | 31-OCT-2017 (Created)
26-JAN-2023 (Last updated, Version 8) |
| Name | Carnitine_monoox_B |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | D0C9N8 (CNTB_ACIB2); F0KFI7 (CNTB_ACICP); P76254 (CNTB_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_02098 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | CNTB |
| Protein name | RecName: Full=Carnitine monooxygenase reductase subunit; EC=1.14.13.239; AltName: Full=Carnitine monooxygenase beta subunit; |
Comments
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| FUNCTION | Converts carnitine to trimethylamine and malic semialdehyde. |
| CATALYTIC ACTIVITY | Reaction=(R)-carnitine + H(+) + NADH + O2 = (3R)-3-hydroxy-4- oxobutanoate + H2O + NAD(+) + trimethylamine; Xref=Rhea:RHEA:55396, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16347, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58389, ChEBI:CHEBI:138809; EC=1.14.13.239; |
| CATALYTIC ACTIVITY | Reaction=(R)-carnitine + H(+) + NADPH + O2 = (3R)-3-hydroxy-4- oxobutanoate + H2O + NADP(+) + trimethylamine; Xref=Rhea:RHEA:55368, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16347, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:58389, ChEBI:CHEBI:138809; EC=1.14.13.239; |
| COFACTOR | Name=FMN; Xref=ChEBI:CHEBI:58210; |
| case <FTGroup:1> | |
| COFACTOR | Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Note=Binds 1 2Fe-2S cluster |
| end case | |
| PATHWAY | Amine and polyamine metabolism; carnitine metabolism. |
| SUBUNIT | Composed of an oxygenase subunit and a reductase subunit. |
| SIMILARITY | Belongs to the PDR/VanB family. CntB subfamily. |
Keywords
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| case <FTGroup:1> | |
| 2Fe-2S | |
| Iron-sulfur | |
| Iron | |
| Metal-binding | |
| end case | |
| Flavoprotein | |
| FMN | |
| NAD | |
| NADP | |
| Oxidoreductase | |
Gene Ontology
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| GO:0016709; Molecular function:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen | |
| case <FTGroup:1> | |
| GO:0051537; Molecular function:2 iron, 2 sulfur cluster binding | |
| end case | |
Cross-references
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| PROSITE | PS00197; 2FE2S_FER_1; 1; |
| PROSITE | PS51085; 2FE2S_FER_2; 1; |
| PROSITE | PS51384; FAD_FR; 1; |
| Pfam | PF00970; FAD_binding_6; 1; |
| Pfam | PF00111; Fer2; 1; |
| Pfam | PF00175; NAD_binding_1; 1; |
| PRINTS | PR00409; PHDIOXRDTASE; 1; |
Features
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| From: CNTB_ACIB2 (D0C9N8) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 267 | 267 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
C | 1 | |||||||
| BINDING | 272 | 272 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
C | 1 | |||||||
| BINDING | 275 | 275 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
C | 1 | |||||||
| BINDING | 305 | 305 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
C | 1 | |||||||
Additional information
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| Size range | 285-330 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | See MF_02097 for the oxygenase subunit. |