HAMAP rule MF_02112
General rule information
[?]
Accession | MF_02112 |
Dates | 2-JUL-2010 (Created) 13-JAN-2023 (Last updated, Version 12) |
Name | ARC_ATPase |
Scope | Bacteria; Actinomycetota |
Templates | P9WQN5 (ARC_MYCTU); O50202 (ARC_RHOER): [Recover all] |
Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier |
|
case <OC:Mycobacteriaceae> and <FTTag:Cterminus>
Protein name |
|
Gene name |
|
end case
case not <OC:Mycobacteriaceae> and <FTTag:Cterminus>
Protein name |
|
Gene name |
|
end case
case not <FTTag:Cterminus>
Protein name |
|
Gene name |
|
end case
Comments
[?]
case <FTTag:Cterminus>
Function | ATPase which is responsible for recognizing, binding, unfolding and translocation of pupylated proteins into the bacterial 20S proteasome core particle. May be essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase may function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. |
Pathway | Protein degradation; proteasomal Pup-dependent pathway. |
Subunit | Homohexamer. Assembles into a hexameric ring structure that caps the 20S proteasome core. Strongly interacts with the prokaryotic ubiquitin-like protein Pup through a hydrophobic interface; the interacting region of ARC lies in its N-terminal coiled-coil domain. There is one Pup binding site per ARC hexamer ring. Upon ATP-binding, the C-terminus of ARC interacts with the alpha-rings of the proteasome core, possibly by binding to the intersubunit pockets. |
Domain | Consists of three main regions, an N-terminal coiled-coil domain that binds to protein Pup and functions as a docking station, an interdomain involved in ARC hexamerization, and a C-terminal ATPase domain of the AAA type. |
else
Subunit | Homohexamer. Assembles into a hexameric ring structure. |
end case
Similarity | Belongs to the AAA ATPase family. |
Keywords
[?]
case <FTTag:Cterminus>
end case
Gene Ontology
[?]
GO:0005524; Molecular function: ATP binding.
GO:0016887; Molecular function: ATP hydrolysis activity.
GO:0016887; Molecular function: ATP hydrolysis activity.
case <FTTag:Cterminus>
GO:0032182; Molecular function: ubiquitin-like protein binding.
GO:0010498; Biological process: proteasomal protein catabolic process.
GO:0019941; Biological process: modification-dependent protein catabolic process.
GO:0043335; Biological process: protein unfolding.
GO:0000502; Cellular component: proteasome complex.
GO:0022623; Cellular component: proteasome-activating nucleotidase complex.
GO:0010498; Biological process: proteasomal protein catabolic process.
GO:0019941; Biological process: modification-dependent protein catabolic process.
GO:0043335; Biological process: protein unfolding.
GO:0000502; Cellular component: proteasome complex.
GO:0022623; Cellular component: proteasome-activating nucleotidase complex.
end case
Cross-references
[?]
Computed features
[?]
General | Coiled_coil; -; 0-unlimited; trigger=yes; |
Features
[?]
From: ARC_MYCTU (P9WQN5) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 296 | 301 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G-x-G-K-T-[LM] | ||||||||
REGION | 608 | 609 | Docks into pockets in the proteasome alpha-ring | Cterminus | Y-x |
Additional information
[?]
Size range | 509-615 amino acids |
Related rules | None |
Fusion | None |
Comments | The protein performing the same function in archaea (called PAN) is in MF_00553. Some actinobacteria having a match in this family do not possess proteasome subunit sequences. The family members from these actinobacteria appear not to have the conserved C-terminus probably important for association with the proteasome, so the rule triggers a different annotation based on this fact. |