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HAMAP rule MF_02113

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General rule information [?]

Accession MF_02113
Dates 2-JUL-2010 (Created)
1-JUN-2023 (Last updated, Version 18)
Name Proteasome_B
Scope(s) Archaea
Bacteria
Actinomycetota
Template(s) P28061 (PSB_THEAC); P9WHT9 (PSB_MYCTU); Q9P996 (PSB_ARCFU); Q58634 (PSB_METJA); D4GYZ1 (PSB_HALVD); Q53079 (PSB1_RHOER); Q53083 (PSB2_RHOER); [ Recover all ]
Triggered by
case c? <OC:Bacteria>
HAMAP; MF_02113_B (Get profile general information and statistics)
end case
case c? <OC:Archaea>
HAMAP; MF_02113_A (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PSB
case <OC:Bacteria>
Protein name RecName: Full=Proteasome subunit beta;
                 EC=3.4.25.1;
AltName: Full=20S proteasome beta subunit;
AltName: Full=Proteasome core protein PrcB;
                 Flags: Precursor;
Gene name Name=prcB;
end case
case <OC:Archaea>
Protein name RecName: Full=Proteasome subunit beta;
                 EC=3.4.25.1;
AltName: Full=20S proteasome beta subunit;
AltName: Full=Proteasome core protein PsmB;
                 Flags: Precursor;
Gene name Name=psmB;
end case

Comments [?]

FUNCTIONComponent of the proteasome core, a large protease complex with broad specificity involved in protein degradation.
CATALYTIC ACTIVITY Reaction=Cleavage of peptide bonds with very broad specificity.; EC=3.4.25.1;
case <OC:Bacteria>
ACTIVITY REGULATIONThe formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.
PATHWAYProtein degradation; proteasomal Pup-dependent pathway.
SUBUNITThe 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.
end case
case <OC:Archaea>
ACTIVITY REGULATIONThe formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close- gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.
SUBUNITThe 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.
end case
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the peptidase T1B family.

Keywords [?]


Gene Ontology [?]

GO:0004298; Molecular function:threonine-type endopeptidase activity
GO:0010498; Biological process:proteasomal protein catabolic process
case <OCellular component:Bacteria>
GO:0019941; Biological process:modification-dependent protein catabolic process
end case
GO:0005737; Cellular component:cytoplasm
GO:0019774; Cellular component:proteasome core complex, beta-subunit complex

Cross-references [?]

PROSITE PS00854; PROTEASOME_BETA_1; 1;
PROSITE PS51476; PROTEASOME_BETA_2; 1;
Pfam PF00227; Proteasome; 1;
PRINTS PR00141; PROTEASOME; 1;
NCBIfam TIGR03634; Arc_protsome_B; 1;
NCBIfam TIGR03690; 20S_bact_beta; 1;

Features [?]

From: PSB_THEAC (P28061)
Key From To Description Tag Condition FTGroup
case <OC:Archaea>
PROPEP Nter 8 /note="Removed in mature form; by autocatalysis"
CHAIN 9 Cter /note="Proteasome subunit beta"
ACT_SITE 9 9 /note="Nucleophile" T
end case
From: PSB_MYCTU (P9WHT9)
Key From To Description Tag Condition FTGroup
case <OC:Bacteria>
PROPEP Nter 57 /note="Removed in mature form; by autocatalysis"
CHAIN 58 Cter /note="Proteasome subunit beta"
ACT_SITE 58 58 /note="Nucleophile" T
end case

Additional information [?]

Size range 193-243 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Subunit alpha of the proteasome core is in MF_00289.



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