HAMAP rule MF_02113

General rule information [?]

Accession	MF_02113
Dates	2-JUL-2010 (Created) 1-JUN-2023 (Last updated, Version 18)
Name	Proteasome_B
Scope(s)	Archaea Bacteria Actinomycetota
Template(s)	P28061 (PSB_THEAC); P9WHT9 (PSB_MYCTU); Q9P996 (PSB_ARCFU); Q58634 (PSB_METJA); D4GYZ1 (PSB_HALVD); Q53079 (PSB1_RHOER); Q53083 (PSB2_RHOER); [ Recover all ]
Triggered by	case c? <OC:Bacteria> HAMAP; MF_02113_B (Get profile general information and statistics) end case case c? <OC:Archaea> HAMAP; MF_02113_A (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	PSB
case <OC:Bacteria>
Protein name	RecName: Full=Proteasome subunit beta; EC=3.4.25.1; AltName: Full=20S proteasome beta subunit; AltName: Full=Proteasome core protein PrcB; Flags: Precursor;
Gene name	Name=prcB;
end case
case <OC:Archaea>
Protein name	RecName: Full=Proteasome subunit beta; EC=3.4.25.1; AltName: Full=20S proteasome beta subunit; AltName: Full=Proteasome core protein PsmB; Flags: Precursor;
Gene name	Name=psmB;
end case

Comments [?]

FUNCTION	Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.
CATALYTIC ACTIVITY	Reaction=Cleavage of peptide bonds with very broad specificity.; EC=3.4.25.1;
case <OC:Bacteria>
ACTIVITY REGULATION	The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.
PATHWAY	Protein degradation; proteasomal Pup-dependent pathway.
SUBUNIT	The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.
end case
case <OC:Archaea>
ACTIVITY REGULATION	The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close- gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.
SUBUNIT	The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.
end case
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the peptidase T1B family.

Keywords [?]

Autocatalytic cleavage

Gene Ontology [?]

GO:0004298; Molecular function:threonine-type endopeptidase activity
GO:0010498; Biological process:proteasomal protein catabolic process
case <OCellular component:Bacteria>
GO:0019941; Biological process:modification-dependent protein catabolic process
end case
GO:0005737; Cellular component:cytoplasm
GO:0019774; Cellular component:proteasome core complex, beta-subunit complex

Cross-references [?]

PROSITE	PS00854; PROTEASOME_BETA_1; 1;
PROSITE	PS51476; PROTEASOME_BETA_2; 1;
Pfam	PF00227; Proteasome; 1;
PRINTS	PR00141; PROTEASOME; 1;
NCBIfam	TIGR03634; Arc_protsome_B; 1;
NCBIfam	TIGR03690; 20S_bact_beta; 1;

Features [?]

From: PSB_THEAC (P28061)

Key

From

Description

Tag

Condition

FTGroup

case <OC:Archaea>

PROPEP

Nter

/note="Removed in mature form; by autocatalysis"

CHAIN

Cter

/note="Proteasome subunit beta"

ACT_SITE

/note="Nucleophile"

end case

From: PSB_MYCTU (P9WHT9)

Key