HAMAP logo

HAMAP rule MF_02118

Send feedback

General rule information [?]

Accession MF_02118
Dates 23-MAY-2011 (Created)
13-JAN-2023 (Last updated, Version 7)
Name LipM
Scope(s) Bacteria
Template(s) P54511 (LIPM_BACSU); [ Recover all ]
Triggered by HAMAP; MF_02118 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier LIPM
Protein name RecName: Full=Octanoyltransferase LipM;
AltName: Full=Octanoyl-[acyl-carrier-protein]:[GcvH] N-octanoyltransferase;
Gene name Name=lipM;

Comments [?]

FUNCTIONCatalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domain of GcvH, an intermediate carrier during protein lipoylation.
CATALYTIC ACTIVITY Reaction=L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)- octanoyl-L-lysyl-[protein]; Xref=Rhea:RHEA:17665, Rhea:RHEA- COMP:9636, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:9752, Rhea:RHEA- COMP:9928, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:64479, ChEBI:CHEBI:78463, ChEBI:CHEBI:78809; EC=;
PATHWAYProtein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein].
MISCELLANEOUSIn the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes. The reaction proceeds via an octanoyl-thioester enzyme intermediate.
SIMILARITYBelongs to the octanoyltransferase LipM family.

Keywords [?]

Gene Ontology [?]

GO:0016415; Molecular function:octanoyltransferase activity
GO:0009107; Biological process:lipoate biosynthetic process
GO:0009249; Biological process:protein lipoylation

Cross-references [?]

Pfam PF03099; BPL_LplA_LipB; 1;

Features [?]

From: LIPM_BACSU (P54511)
Key From To Description Tag Condition FTGroup
ACT_SITE 150 150 /note="Acyl-thioester intermediate" C
SITE 165 165 /note="Lowers pKa of active site Cys" K

Additional information [?]

Size range 269-278 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments E.coli requires only 2 proteins for the endogenous pathway of protein lipoylation, LipB (MF_00013) and LipA (MF_00206), whereas B.subtilis (and probably many Bacillota) requires 3 enzymes: LipM (MF_02118), LipL (MF_02119) and LipA (MF_00206).

View rule in raw text format (no links)