HAMAP rule MF_02118
General rule information
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Accession | MF_02118 |
Dates | 23-MAY-2011 (Created) 13-JAN-2023 (Last updated, Version 7) |
Name | LipM |
Scope | Bacteria; Bacillota |
Template | P54511 (LIPM_BACSU) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domain of GcvH, an intermediate carrier during protein lipoylation. |
Catalytic activity | RHEA:17665: L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)-octanoyl-L-lysyl-[protein]
EC 2.3.1.181 |
Pathway | Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]. |
Subunit | Monomer. |
Miscellaneous | In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes. The reaction proceeds via an octanoyl-thioester enzyme intermediate. |
Similarity | Belongs to the octanoyltransferase LipM family. |
Keywords
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Gene Ontology
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GO:0016415; Molecular function: octanoyltransferase activity.
GO:0009107; Biological process: lipoate biosynthetic process.
GO:0009249; Biological process: protein lipoylation.
GO:0009107; Biological process: lipoate biosynthetic process.
GO:0009249; Biological process: protein lipoylation.
Cross-references
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Features
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From: LIPM_BACSU (P54511) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 150 | 150 | Acyl-thioester intermediate | C | ||||||||
SITE | 165 | 165 | Lowers pKa of active site Cys | K |
Additional information
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Size range | 269-278 amino acids |
Related rules | None |
Fusion | None |
Comments | E.coli requires only 2 proteins for the endogenous pathway of protein lipoylation, LipB (MF_00013) and LipA (MF_00206), whereas B.subtilis (and probably many Bacillota) requires 3 enzymes: LipM (MF_02118), LipL (MF_02119) and LipA (MF_00206). |