HAMAP rule MF_02124
General rule information
[?]
| PURL | https://purl.expasy.org/hamap/rule/MF_02124 |
| Accession | MF_02124 |
| Dates | 29-SEP-2011 (Created)
2-SEP-2024 (Last updated, Version 13) |
| Name | GlgE |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P9WQ17 (GLGE_MYCTU); Q9L1K2 (GLGE1_STRCO); Q9RP48 (GLGE_MYCS2); Q9KY04 (GLGE2_STRCO); [ Recover all ] |
| Triggered by |
HAMAP; MF_02124 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | GLGE |
| Protein name | RecName: Full=Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase; Short=GMPMT; EC=2.4.99.16; AltName: Full=(1->4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase; |
| Gene name | Name=glgE; |
Comments
[?]
| FUNCTION | Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB. |
| CATALYTIC ACTIVITY | Reaction=alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl](n) = [(1->4)-alpha-D-glucosyl](n+2) + phosphate; Xref=Rhea:RHEA:42692, Rhea:RHEA-COMP:9584, Rhea:RHEA-COMP:10183, ChEBI:CHEBI:15444, ChEBI:CHEBI:43474, ChEBI:CHEBI:63576; EC=2.4.99.16; |
| case <OC:Mycobacterium> | |
| PATHWAY | Glycan biosynthesis; glycogen biosynthesis. |
| end case | |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the glycosyl hydrolase 13 family. GlgE subfamily. |
Keywords
[?]
| Carbohydrate metabolism | |
| Glycosyltransferase | |
| Transferase | |
| case <OC:Mycobacterium> | |
| Glycogen biosynthesis | |
| Glycogen metabolism | |
| end case | |
Gene Ontology
[?]
| GO:0016758; Molecular function:hexosyltransferase activity | |
| GO:0030979; Biological process:alpha-glucan biosynthetic process | |
| case <OCellular component:Mycobacterium> | |
| GO:0005978; Biological process:glycogen biosynthetic process | |
| end case | |
Cross-references
[?]
Features
[?]
| From: GLGE1_STRCO (Q9L1K2) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 534 | 535 | /ligand="alpha-maltose 1-phosphate" /ligand_id="ChEBI:CHEBI:63576" |
K-[YF] | ||||||||
| ACT_SITE | 394 | 394 | /note="Nucleophile" | D | ||||||||
| ACT_SITE | 423 | 423 | /note="Proton donor" | E | ||||||||
| BINDING | 264 | 264 | /ligand="alpha-maltose 1-phosphate" /ligand_id="ChEBI:CHEBI:63576" |
[KR] | ||||||||
| BINDING | 324 | 324 | /ligand="alpha-maltose 1-phosphate" /ligand_id="ChEBI:CHEBI:63576" |
Q | ||||||||
| BINDING | 359 | 359 | /ligand="alpha-maltose 1-phosphate" /ligand_id="ChEBI:CHEBI:63576" |
D | ||||||||
| BINDING | 395 | 395 | /ligand="alpha-maltose 1-phosphate" /ligand_id="ChEBI:CHEBI:63576" |
N | ||||||||
| SITE | 480 | 480 | /note="Transition state stabilizer" | D | ||||||||
Additional information
[?]
| Size range | 630-746 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | The "GlgE pathway" has been shown to be involved in the conversion of trehalosee to glycogen in M.smegmatis (see PubMed=18505459), but it may be also involved in the biosynthesis of other types of alpha(1->4)-glucans. |