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HAMAP rule MF_02124

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General rule information [?]

Accession MF_02124
Dates 29-SEP-2011 (Created)
19-NOV-2022 (Last updated, Version 12)
Name GlgE
Templates P9WQ17 (GLGE_MYCTU); Q9L1K2 (GLGE1_STRCO); Q9RP48 (GLGE_MYCS2); Q9KY04 (GLGE2_STRCO): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase;
AltName: Full=(1->4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase;
Gene name

Comments [?]

Function Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.
Catalytic activity RHEA:42692: [(1->4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate = [(1->4)-alpha-D-glucosyl](n+2) + phosphate
case <OC:Mycobacterium>
Pathway Glycan biosynthesis; glycogen biosynthesis.
end case
Subunit Homodimer.
Similarity Belongs to the glycosyl hydrolase 13 family. GlgE subfamily.

Keywords [?]

case <OC:Mycobacterium>
end case

Gene Ontology [?]

GO:0016758; Molecular function: hexosyltransferase activity.
GO:0030979; Biological process: alpha-glucan biosynthetic process.
case <OC:Mycobacterium>
GO:0005978; Biological process: glycogen biosynthetic process.
end case

Cross-references [?]

Pfam PF00128; Alpha-amylase; 1;
PF11896; DUF3416; 1;

Features [?]

From: GLGE1_STRCO (Q9L1K2)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     534     535       /ligand="alpha-maltose 1-phosphate" /ligand_id="ChEBI:CHEBI:63576     K-[YF]  
ACT_SITE     394     394       Nucleophile     D  
ACT_SITE     423     423       Proton donor     E  
BINDING     264     264       /ligand="alpha-maltose 1-phosphate" /ligand_id="ChEBI:CHEBI:63576     [KR]  
BINDING     324     324       /ligand="alpha-maltose 1-phosphate" /ligand_id="ChEBI:CHEBI:63576     Q  
BINDING     359     359       /ligand="alpha-maltose 1-phosphate" /ligand_id="ChEBI:CHEBI:63576     D  
BINDING     395     395       /ligand="alpha-maltose 1-phosphate" /ligand_id="ChEBI:CHEBI:63576     N  
SITE     480     480       Transition state stabilizer     D  

Additional information [?]

Size range 630-746 amino acids
Related rules None
Fusion None
Comments The "GlgE pathway" has been shown to be involved in the conversion of trehalosee to glycogen in M.smegmatis (see PubMed=18505459), but it may be also involved in the biosynthesis of other types of alpha(1->4)-glucans.