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HAMAP rule MF_02124

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General rule information [?]

Accession MF_02124
Dates 29-SEP-2011 (Created)
19-NOV-2022 (Last updated, Version 12)
Name GlgE
Scope(s) Bacteria
Template(s) P9WQ17 (GLGE_MYCTU); Q9L1K2 (GLGE1_STRCO); Q9RP48 (GLGE_MYCS2); Q9KY04 (GLGE2_STRCO); [ Recover all ]
Triggered by HAMAP; MF_02124 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GLGE
Protein name RecName: Full=Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase;
AltName: Full=(1->4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase;
Gene name Name=glgE;

Comments [?]

FUNCTIONMaltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.
CATALYTIC ACTIVITY Reaction=[(1->4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate = [(1->4)-alpha-D-glucosyl](n+2) + phosphate; Xref=Rhea:RHEA:42692, Rhea:RHEA-COMP:9584, Rhea:RHEA-COMP:10183, ChEBI:CHEBI:15444, ChEBI:CHEBI:43474, ChEBI:CHEBI:63576; EC=;
case <OC:Mycobacterium>
PATHWAYGlycan biosynthesis; glycogen biosynthesis.
end case
SIMILARITYBelongs to the glycosyl hydrolase 13 family. GlgE subfamily.

Keywords [?]

Gene Ontology [?]

GO:0016758; Molecular function:hexosyltransferase activity
GO:0030979; Biological process:alpha-glucan biosynthetic process
case <OCellular component:Mycobacterium>
GO:0005978; Biological process:glycogen biosynthetic process
end case

Cross-references [?]

Pfam PF00128; Alpha-amylase; 1;
Pfam PF11896; DUF3416; 1;

Features [?]

From: GLGE1_STRCO (Q9L1K2)
Key From To Description Tag Condition FTGroup
BINDING 534 535 /ligand="alpha-maltose 1-phosphate"
ACT_SITE 394 394 /note="Nucleophile" D
ACT_SITE 423 423 /note="Proton donor" E
BINDING 264 264 /ligand="alpha-maltose 1-phosphate"
BINDING 324 324 /ligand="alpha-maltose 1-phosphate"
BINDING 359 359 /ligand="alpha-maltose 1-phosphate"
BINDING 395 395 /ligand="alpha-maltose 1-phosphate"
SITE 480 480 /note="Transition state stabilizer" D

Additional information [?]

Size range 630-746 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments The "GlgE pathway" has been shown to be involved in the conversion of trehalosee to glycogen in M.smegmatis (see PubMed=18505459), but it may be also involved in the biosynthesis of other types of alpha(1->4)-glucans.

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