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HAMAP rule MF_02214

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General rule information [?]

Accession MF_02214
Dates 7-MAR-2019 (Created)
19-NOV-2022 (Last updated, Version 6)
Name Lipid_II_synth_MurT
Scope(s) Bacteria
Template(s) A0A0H2WZQ7 (MURT_STAAC); A0A0H3JUU7 (MURT_STAAN); Q8DNZ9 (MURT_STRR6); [ Recover all ]
Triggered by HAMAP; MF_02214 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MURT
Protein name RecName: Full=Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT;
Gene name Name=murT;

Comments [?]

FUNCTIONThe lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D- glutamate residue of lipid II, converting it to an isoglutamine residue.
CATALYTIC ACTIVITY Reaction=ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys- D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L- glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D- isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:57928, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:60033, ChEBI:CHEBI:62233, ChEBI:CHEBI:456216; EC=;
CATALYTIC ACTIVITY Reaction=ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys- D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = ADP + beta- D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)- di-trans,octa-cis-undecaprenyl diphosphate; Xref=Rhea:RHEA:59488, ChEBI:CHEBI:30616, ChEBI:CHEBI:60033, ChEBI:CHEBI:143132, ChEBI:CHEBI:456216;
CATALYTIC ACTIVITY Reaction=beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D- Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4(+) = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D- Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + phosphate; Xref=Rhea:RHEA:57932, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:43474, ChEBI:CHEBI:62233, ChEBI:CHEBI:143132;
PATHWAYCell wall biogenesis; peptidoglycan biosynthesis.
SUBUNITForms a heterodimer with GatD.
SIMILARITYBelongs to the MurCDEF family. MurT subfamily.

Keywords [?]

Gene Ontology [?]

GO:0140282; Molecular function:carbon-nitrogen ligase activity on lipid II
GO:0005524; Molecular function:ATP binding
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case
GO:0009252; Biological process:peptidoglycan biosynthetic process

Cross-references [?]

Pfam PF08353; DUF1727; 1;
Pfam PF08245; Mur_ligase_M; 1;

Features [?]

Key From To Description Tag Condition FTGroup
ACT_SITE 349 349 D
BINDING 202 202 /ligand="Zn(2+)"
C 1
BINDING 205 205 /ligand="Zn(2+)"
C 1
BINDING 224 224 /ligand="Zn(2+)"
C 1
BINDING 226 226 /ligand="Zn(2+)"
C 1

Additional information [?]

Size range 370-550 amino acids
Related rules None
Fusion Nter: None Cter: None

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