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HAMAP rule MF_02214

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General rule information [?]

Accession MF_02214
Dates 7-MAR-2019 (Created)
19-NOV-2022 (Last updated, Version 6)
Name Lipid_II_synth_MurT
Templates A0A0H2WZQ7 (MURT_STAAC); A0A0H3JUU7 (MURT_STAAN); Q8DNZ9 (MURT_STRR6): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT;
Gene name

Comments [?]

Function The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue.
Catalytic activity RHEA:57928: ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate
RHEA:59488: ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate
RHEA:57932: beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4(+) = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + phosphate
Pathway Cell wall biogenesis; peptidoglycan biosynthesis.
Subunit Forms a heterodimer with GatD.
Similarity Belongs to the MurCDEF family. MurT subfamily.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0140282; Molecular function: carbon-nitrogen ligase activity on lipid II.
GO:0005524; Molecular function: ATP binding.
case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0009252; Biological process: peptidoglycan biosynthetic process.

Cross-references [?]

Pfam PF08353; DUF1727; 1;
PF08245; Mur_ligase_M; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     349     349             D  
BINDING     202     202       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C   1
BINDING     205     205       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C   1
BINDING     224     224       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C   1
BINDING     226     226       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C   1

Additional information [?]

Size range 370-550 amino acids
Related rules None
Fusion None