HAMAP rule MF_02214
General rule information
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| Accession | MF_02214 |
| Dates | 7-MAR-2019 (Created)
2-SEP-2024 (Last updated, Version 7) |
| Name | Lipid_II_synth_MurT |
| Scope(s) |
Bacteria Archaea |
| Template(s) | A0A0H2WZQ7 (MURT_STAAC); A0A0H3JUU7 (MURT_STAAN); Q8DNZ9 (MURT_STRR6); [ Recover all ] |
| Triggered by |
HAMAP; MF_02214 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MURT |
| Protein name | RecName: Full=Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT; EC=6.3.5.13; |
| Gene name | Name=murT; |
Comments
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| FUNCTION | The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D- glutamate residue of lipid II, converting it to an isoglutamine residue. |
| CATALYTIC ACTIVITY | Reaction=beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala- D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + L-glutamine + ATP + H2O = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys- D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + L-glutamate + ADP + phosphate + H(+); Xref=Rhea:RHEA:57928, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:60033, ChEBI:CHEBI:62233, ChEBI:CHEBI:456216; EC=6.3.5.13; |
| CATALYTIC ACTIVITY | Reaction=beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala- D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + ATP = beta-D- GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di- trans,octa-cis-undecaprenyl diphosphate + ADP; Xref=Rhea:RHEA:59488, ChEBI:CHEBI:30616, ChEBI:CHEBI:60033, ChEBI:CHEBI:143132, ChEBI:CHEBI:456216; |
| CATALYTIC ACTIVITY | Reaction=beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D- Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4(+) = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D- Ala)-di-trans,octa-cis-undecaprenyl diphosphate + phosphate + H(+); Xref=Rhea:RHEA:57932, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:43474, ChEBI:CHEBI:62233, ChEBI:CHEBI:143132; |
| PATHWAY | Cell wall biogenesis; peptidoglycan biosynthesis. |
| SUBUNIT | Forms a heterodimer with GatD. |
| SIMILARITY | Belongs to the MurCDEF family. MurT subfamily. |
Keywords
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| ATP-binding | |
| Cell shape | |
| Cell wall biogenesis/degradation | |
| Ligase | |
| Metal-binding | |
| Nucleotide-binding | |
| Peptidoglycan synthesis | |
| case <FTGroup:1> | |
| Zinc | |
| end case | |
Gene Ontology
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| GO:0140282; Molecular function:carbon-nitrogen ligase activity on lipid II | |
| GO:0005524; Molecular function:ATP binding | |
| case <FTGroup:1> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0009252; Biological process:peptidoglycan biosynthetic process | |
Cross-references
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Features
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| From: MURT_STAAN (A0A0H3JUU7) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 349 | 349 | D | |||||||||
| BINDING | 202 | 202 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 205 | 205 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 224 | 224 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 226 | 226 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
Additional information
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| Size range | 370-550 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |