HAMAP rule MF_02214

General rule information [?]

Accession	MF_02214
Dates	7-MAR-2019 (Created) 19-NOV-2022 (Last updated, Version 6)

Name	Lipid_II_synth_MurT

Scope

Bacteria

Archaea

Templates

A0A0H2WZQ7 (MURT_STAAC); A0A0H3JUU7 (MURT_STAAN); Q8DNZ9 (MURT_STRR6): [Recover all]

Triggered by

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

MURT

Protein name

RecName:		Full=Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT;
		EC 6.3.5.13;

Gene name

murT

Comments [?]

Function	The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue.
Catalytic activity	RHEA:57928: ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate EC 6.3.5.13
	RHEA:59488: ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate
	RHEA:57932: beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4(+) = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + phosphate
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subunit	Forms a heterodimer with GatD.
Similarity	Belongs to the MurCDEF family. MurT subfamily.

Keywords [?]

case <FTGroup:1>

Zinc

end case

Gene Ontology [?]

GO:0140282; Molecular function: carbon-nitrogen ligase activity on lipid II.
GO:0005524; Molecular function: ATP binding.

case <FTGroup:1>

GO:0008270; Molecular function: zinc ion binding.

end case

GO:0009252; Biological process: peptidoglycan biosynthetic process.

Cross-references [?]

Pfam	PF08353; DUF1727; 1;
	PF08245; Mur_ligase_M; 1;

Features [?]

From: MURT_STAAN (A0A0H3JUU7)

Key From To Description Tag Condition FTGroup

ACT_SITE 349 349 D

BINDING 202 202 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C 1

BINDING 205 205 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C 1

BINDING 224 224 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C 1

BINDING 226 226 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C 1

Additional information [?]