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HAMAP rule MF_02232

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_02232
Accession MF_02232
Dates 01-OCT-2019 (Created)
08-APR-2025 (Last updated, Version 8)
Name UbiU
Scope(s) Bacteria
Pseudomonadota
Template(s) P45527; [ Recover all ]
Triggered by HAMAP; MF_02232 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier UBIU
Protein name RecName: Full=Ubiquinone biosynthesis hydroxylase UbiU;
                 EC=1.97.1.15;
AltName: Full=2-(all-trans-polyprenyl)phenol 6-hydroxylase subunit UbiU;
Gene name Name=ubiU;

Comments [?]

FUNCTIONComponent of the UbiUV hydroxylase, which is involved in the oxygen-independent ubiquinone (coenzyme Q) biosynthesis. It catalyzes the three hydroxylation steps, using prephenate as the oxygen donor.
CATALYTIC ACTIVITY Reaction=a 2-(all-trans-polyprenyl)phenol + prephenate + A + H(+) = a 3-(all-trans-polyprenyl)benzene-1,2-diol + 3-phenylpyruvate + AH2 + CO2; Xref=Rhea:RHEA:81671, Rhea:RHEA-COMP:9516, Rhea:RHEA-COMP:9550, ChEBI:CHEBI:1269, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:17499, ChEBI:CHEBI:18005, ChEBI:CHEBI:29934, ChEBI:CHEBI:62729; EC=1.97.1.15; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:81672;
CATALYTIC ACTIVITY Reaction=a 2-methoxy-6-(all-trans-polyprenyl)phenol + prephenate + A + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + 3- phenylpyruvate + AH2 + CO2; Xref=Rhea:RHEA:81679, Rhea:RHEA- COMP:9551, Rhea:RHEA-COMP:10858, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:17499, ChEBI:CHEBI:18005, ChEBI:CHEBI:29934, ChEBI:CHEBI:62731, ChEBI:CHEBI:84166; EC=1.97.1.15; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:81680;
CATALYTIC ACTIVITY Reaction=a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + prephenate + A + H(+) = a 3-demethylubiquinol + 3-phenylpyruvate + AH2 + CO2; Xref=Rhea:RHEA:81687, Rhea:RHEA-COMP:10859, Rhea:RHEA- COMP:10914, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:17499, ChEBI:CHEBI:18005, ChEBI:CHEBI:29934, ChEBI:CHEBI:84167, ChEBI:CHEBI:84422; EC=1.97.1.15; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:81688;
case <FTGroup:1>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
end case
PATHWAYCofactor biosynthesis; ubiquinone biosynthesis.
SUBUNITForms a heterodimer with UbiV.
SIMILARITYBelongs to the peptidase U32 family. UbiU subfamily.

Keywords [?]

case <FTGroup:1>
4Fe-4S
Iron
Iron-sulfur
Metal-binding
end case
Ubiquinone biosynthesis

Gene Ontology [?]

case <FTGroup:1>
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
end case
GO:0006744; Biological process:ubiquinone biosynthetic process

Cross-references [?]

PROSITE PS01276; PEPTIDASE_U32; 1;
Pfam PF01136; Peptidase_U32; 1;

Features [?]

From: UBIU_ECOLI (P45527)
Key From To Description Tag Condition FTGroup
BINDING 169 169 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C 1
BINDING 176 176 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C 1
BINDING 193 193 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C 1
BINDING 232 232 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C 1

Additional information [?]

Size range 290-400 amino acids
Related rules None
Fusion Nter: None Cter: None



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