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HAMAP rule MF_03001

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General rule information [?]

Accession MF_03001
Dates 29-JAN-2009 (Created)
19-MAR-2021 (Last updated, Version 17)
Name eIF3b
Scope
Eukaryota
Templates P55884 (EIF3B_HUMAN); Q8JZQ9 (EIF3B_MOUSE); Q4G061 (EIF3B_RAT); Q9XWI6 (EIF3B_CAEEL); Q0E940 (EIF3B_DROME); Q10425 (EIF3B_SCHPO); P06103 (EIF3B_YEAST): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
EIF3B
case <OC:Vertebrata>
Protein name
RecName: Full=Eukaryotic translation initiation factor 3 subunit B;
Short=eIF3b;
AltName: Full=Eukaryotic translation initiation factor 3 subunit 9;
AltName: Full=eIF-3-eta;
else case <OC:Fungi>
Protein name
RecName: Full=Eukaryotic translation initiation factor 3 subunit B;
Short=eIF3b;
AltName: Full=Eukaryotic translation initiation factor 3 90 kDa subunit homolog;
Short=eIF3 p90;
AltName: Full=Translation initiation factor eIF3, p90 subunit homolog;
else case <OC:Viridiplantae>
Protein name
RecName: Full=Eukaryotic translation initiation factor 3 subunit B;
Short=eIF3b;
AltName: Full=eIF-3-eta;
AltName: Full=eIF3 p110;
else
Protein name
RecName: Full=Eukaryotic translation initiation factor 3 subunit B;
Short=eIF3b;
AltName: Full=Eukaryotic translation initiation factor 3 subunit 9;
end case
case <OC:Vertebrata>
Gene name
EIF3B, EIF3S9
else case <OC:Caenorhabditis>
Gene name
eif-3.B
else case <OC:Drosophilidae>
Gene name
eIF3b, eIF3-S9
else case <OC:Fungi>
Gene name
PRT1
end case

Comments [?]

case <OC:Mammalia>
Function RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
else
Function RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
end case
case <OC:Mammalia>
Subunit Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with UPF2. Interacts with METTL3.
else case <OC:Vertebrata> and not <OC:Mammalia>
Subunit Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: @gn(EIF3A), @gn(EIF3B), @gn(EIF3C), @gn(EIF3D), @gn(EIF3E), @gn(EIF3F), @gn(EIF3G), @gn(EIF3H), @gn(EIF3I), @gn(EIF3J), @gn(EIF3K), @gn(EIF3L) and @gn(EIF3M).
else case <OC:Drosophilidae>
Subunit Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.
else
Subunit Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.
end case
Subcellular location Cytoplasm.
case <OC:Mammalia>
Domain The RRM domain mediates interaction with EIF3J.
Ptm Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
end case
Similarity Belongs to the eIF-3 subunit B family.

Keywords [?]

case <FTTag:acetyl>
Acetylation
end case
Cytoplasm
Initiation factor
case <OC:Mammalia>
Phosphoprotein
end case
Protein biosynthesis
RNA-binding

Gene Ontology [?]

GO:0003723; Molecular function: RNA binding.
GO:0003743; Molecular function: translation initiation factor activity.
GO:0001732; Biological process: formation of cytoplasmic translation initiation complex.
GO:0002183; Biological process: cytoplasmic translational initiation.
GO:0005737; Cellular component: cytoplasm.
GO:0005852; Cellular component: eukaryotic translation initiation factor 3 complex.
GO:0016282; Cellular component: eukaryotic 43S preinitiation complex.
GO:0033290; Cellular component: eukaryotic 48S preinitiation complex.

Cross-references [?]

PROSITE PS50102; RRM; 1; trigger=PRU00176;
PS00678; WD_REPEATS_1; 1;
PS50082; WD_REPEATS_2; 1;
PS50294; WD_REPEATS_REGION; 1;
Pfam PF08662; eIF2A; 1;
PF00076; RRM_1; 1;

Computed features [?]

REP Repeat_WD40; WD40; 5-7;

Features [?]

case <OC:Mammalia>
From: EIF3B_HUMAN (P55884)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     124     413       Sufficient for interaction with EIF3E        
REGION     170     274       Sufficient for interaction with EIF3J        
MOD_RES     1     1       N-acetylmethionine   acetyl   M  
MOD_RES     83     83       Phosphoserine     S  
MOD_RES     85     85       Phosphoserine     S  
MOD_RES     119     119       Phosphoserine     S  
MOD_RES     125     125       Phosphoserine     S  
MOD_RES     152     152       Phosphoserine     S  
MOD_RES     154     154       Phosphoserine     S  
MOD_RES     164     164       Phosphoserine     S  
end case
case <OC:Saccharomycetes>
From: EIF3B_YEAST (P06103)
REGION     1     136       Sufficient for interaction with HCR1 and TIF32        
REGION     28     261       Sufficient for interaction with PIC8        
end case

Additional information [?]

Size range 621-814 amino acids
Related rules None
Fusion None


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