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HAMAP rule MF_03001

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_03001
Accession MF_03001
Dates 29-JAN-2009 (Created)
19-MAR-2021 (Last updated, Version 18)
Name eIF3b
Scope(s) Eukaryota
Template(s) P55884; Q8JZQ9; Q4G061; Q9XWI6; Q0E940; Q10425; P06103; [ Recover all ]
Triggered by HAMAP; MF_03001 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier EIF3B
case <OC:Vertebrata>
Protein name RecName: Full=Eukaryotic translation initiation factor 3 subunit B;
                 Short=eIF3b;
AltName: Full=Eukaryotic translation initiation factor 3 subunit 9;
AltName: Full=eIF-3-eta;
else case <OC:Fungi>
Protein name RecName: Full=Eukaryotic translation initiation factor 3 subunit B;
                 Short=eIF3b;
AltName: Full=Eukaryotic translation initiation factor 3 90 kDa subunit homolog;
                 Short=eIF3 p90;
AltName: Full=Translation initiation factor eIF3, p90 subunit homolog;
else case <OC:Viridiplantae>
Protein name RecName: Full=Eukaryotic translation initiation factor 3 subunit B;
                 Short=eIF3b;
AltName: Full=eIF-3-eta;
AltName: Full=eIF3 p110;
else
Protein name RecName: Full=Eukaryotic translation initiation factor 3 subunit B;
                 Short=eIF3b;
AltName: Full=Eukaryotic translation initiation factor 3 subunit 9;
end case
case <OC:Vertebrata>
Gene name Name=EIF3B; Synonyms=EIF3S9;
else case <OC:Caenorhabditis>
Gene name Name=eif-3.B;
else case <OC:Drosophilidae>
Gene name Name=eIF3b; Synonyms=eIF3-S9;
else case <OC:Fungi>
Gene name Name=PRT1;
end case

Comments [?]

case <OC:Mammalia>
FUNCTIONRNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
else
FUNCTIONRNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
end case
case <OC:Mammalia>
SUBUNITComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with UPF2. Interacts with METTL3.
else case <OC:Vertebrata> and not <OC:Mammalia>
SUBUNITComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: @gn(EIF3A), @gn(EIF3B), @gn(EIF3C), @gn(EIF3D), @gn(EIF3E), @gn(EIF3F), @gn(EIF3G), @gn(EIF3H), @gn(EIF3I), @gn(EIF3J), @gn(EIF3K), @gn(EIF3L) and @gn(EIF3M).
else case <OC:Drosophilidae>
SUBUNITComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.
else
SUBUNITComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex.
end case
SUBCELLULAR LOCATIONCytoplasm.
case <OC:Mammalia>
DOMAINThe RRM domain mediates interaction with EIF3J.
PTMPhosphorylated. Phosphorylation is enhanced upon serum stimulation.
end case
SIMILARITYBelongs to the eIF-3 subunit B family.

Keywords [?]

case <FTTag:acetyl>
Acetylation
end case
Cytoplasm
Initiation factor
case <OC:Mammalia>
Phosphoprotein
end case
Protein biosynthesis
RNA-binding

Gene Ontology [?]

GO:0003723; Molecular function:RNA binding
GO:0003743; Molecular function:translation initiation factor activity
GO:0001732; Biological process:formation of cytoplasmic translation initiation complex
GO:0002183; Biological process:cytoplasmic translational initiation
GO:0005737; Cellular component:cytoplasm
GO:0005852; Cellular component:eukaryotic translation initiation factor 3 complex
GO:0016282; Cellular component:eukaryotic 43S preinitiation complex
GO:0033290; Cellular component:eukaryotic 48S preinitiation complex

Cross-references [?]

PROSITE PS50102; RRM; 1;
PROSITE PS00678; WD_REPEATS_1; 1;
PROSITE PS50082; WD_REPEATS_2; 1;
PROSITE PS50294; WD_REPEATS_REGION; 1;
Pfam PF08662; eIF2A; 1;
Pfam PF00076; RRM_1; 1;
REP Repeat_WD40; WD40; 5-7;

Features [?]

From: EIF3B_HUMAN (P55884)
Key From To Description Tag Condition FTGroup
case <OC:Mammalia>
REGION 124 413 /note="Sufficient for interaction with EIF3E"
REGION 170 274 /note="Sufficient for interaction with EIF3J"
MOD_RES 1 1 /note="N-acetylmethionine" acetyl M
MOD_RES 83 83 /note="Phosphoserine" S
MOD_RES 85 85 /note="Phosphoserine" S
MOD_RES 119 119 /note="Phosphoserine" S
MOD_RES 125 125 /note="Phosphoserine" S
MOD_RES 152 152 /note="Phosphoserine" S
MOD_RES 154 154 /note="Phosphoserine" S
MOD_RES 164 164 /note="Phosphoserine" S
end case
From: EIF3B_YEAST (P06103)
Key From To Description Tag Condition FTGroup
case <OC:Saccharomycetes>
REGION 1 136 /note="Sufficient for interaction with HCR1 and TIF32"
REGION 28 261 /note="Sufficient for interaction with PIC8"
end case

Additional information [?]

Size range 621-814 amino acids
Related rules None
Fusion Nter: None Cter: None



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