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HAMAP rule MF_03009

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General rule information [?]

Accession MF_03009
Dates 30-JAN-2009 (Created)
12-AUG-2021 (Last updated, Version 16)
Name eIF3j
Scope(s) Eukaryota
Template(s) O75822 (EIF3J_HUMAN); Q66JS6 (EI3JB_MOUSE); Q05775 (EIF3J_YEAST); [ Recover all ]
Triggered by HAMAP; MF_03009 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier EIF3J
case <OC:Vertebrata>
Protein name RecName: Full=Eukaryotic translation initiation factor 3 subunit J;
                 Short=eIF3j;
AltName: Full=Eukaryotic translation initiation factor 3 subunit 1;
AltName: Full=eIF-3-alpha;
AltName: Full=eIF3 p35;
else case <OC:Fungi>
Protein name RecName: Full=Eukaryotic translation initiation factor 3 subunit J;
                 Short=eIF3j;
AltName: Full=Eukaryotic translation initiation factor 3 30 kDa subunit homolog;
                 Short=eIF-3 30 kDa subunit homolog;
else
Protein name RecName: Full=Eukaryotic translation initiation factor 3 subunit J;
                 Short=eIF3j;
end case
case <OC:Vertebrata>
Gene name Name=EIF3J; Synonyms=EIF3S1;
else case <OC:Drosophilidae>
Gene name Name=eIF3j; Synonyms=Adam;
else case <OC:Fungi>
Gene name Name=HCR1;
end case

Comments [?]

case <OC:Mammalia>
FUNCTIONComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF- 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.
else
FUNCTIONComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
end case
case <OC:Mammalia>
SUBUNITComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.
else case <OC:Vertebrata> and not <OC:Mammalia>
SUBUNITComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: @gn(EIF3A), @gn(EIF3B), @gn(EIF3C), @gn(EIF3D), @gn(EIF3E), @gn(EIF3F), @gn(EIF3G), @gn(EIF3H), @gn(EIF3I), @gn(EIF3J), @gn(EIF3K), @gn(EIF3L) and @gn(EIF3M).
else case <OC:Drosophilidae>
SUBUNITComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.
else
SUBUNITComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex.
end case
SUBCELLULAR LOCATIONCytoplasm.
case <OC:Mammalia>
PTMPhosphorylated. Phosphorylation is enhanced upon serum stimulation.
end case
SIMILARITYBelongs to the eIF-3 subunit J family.

Keywords [?]

Cytoplasm
Initiation factor
Protein biosynthesis
case <FTGroup:1>
Acetylation
end case
case <OC:Mammalia>
Phosphoprotein
end case

Gene Ontology [?]

GO:0003743; Molecular function:translation initiation factor activity
GO:0001732; Biological process:formation of cytoplasmic translation initiation complex
GO:0002183; Biological process:cytoplasmic translational initiation
GO:0005737; Cellular component:cytoplasm
GO:0005852; Cellular component:eukaryotic translation initiation factor 3 complex
GO:0016282; Cellular component:eukaryotic 43S preinitiation complex
GO:0033290; Cellular component:eukaryotic 48S preinitiation complex

Cross-references [?]

Pfam PF08597; eIF3_subunit; 1;
General Coiled_coil; -; 0-unlimited;

Features [?]

From: EIF3J_HUMAN (O75822)
Key From To Description Tag Condition FTGroup
case <OC:Mammalia>
INIT_MET Nter Nter /note="Removed" M 1
REGION 1 69 /note="Sufficient for interaction with EIF3B"
REGION 243 258 /note="Promotes stable association with the 40S ribosome"
MOD_RES Nter+1 Nter+1 /note="N-acetylalanine" A 1
MOD_RES 11 11 /note="Phosphoserine" S
MOD_RES 13 13 /note="Phosphoserine" S
MOD_RES 20 20 /note="Phosphoserine" S
MOD_RES 109 109 /note="Phosphothreonine" T
end case

Additional information [?]

Size range 211-289 amino acids
Related rules None
Fusion Nter: None Cter: None



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