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Annotation rule MF_03062 |
Accession | MF_03062 |
Dates | 26-FEB-2009 (Created) 18-AUG-2020 (Last updated, Version 15) |
Name | UBP16 |
Scope | Eukaryota; Vertebrata |
Template | Q9Y5T5 (UBP16_HUMAN) |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. |
Function | Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. |
Catalytic activity | Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
Subunit | Homotetramer. |
Subcellular location | Nucleus. |
Domain | The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin. |
Ptm | Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity. |
Similarity | Belongs to the peptidase C19 family. USP16 subfamily. |
PROSITE | PS00972; USP_1; 1; |
PS00973; USP_2; 1; | |
PS50235; USP_3; 1; | |
PS50271; ZF_UBP; 1; trigger=PRU00502; | |
Pfam | PF00443; UCH; 1; |
PF02148; zf-UBP; 1; |
From: UBP16_HUMAN (Q9Y5T5) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 205 | 205 | Nucleophile | C | ||||||||
ACT_SITE | 758 | 758 | Proton acceptor | H | ||||||||
METAL | 24 | 24 | Zinc 1 | C | ||||||||
METAL | 26 | 26 | Zinc 1 | H | ||||||||
METAL | 48 | 48 | Zinc 2 | C | ||||||||
METAL | 51 | 51 | Zinc 2 | C | ||||||||
METAL | 74 | 74 | Zinc 3 | C | ||||||||
METAL | 77 | 77 | Zinc 3 | C | ||||||||
METAL | 82 | 82 | Zinc 2 | C | ||||||||
METAL | 90 | 90 | Zinc 2 | H | ||||||||
METAL | 94 | 94 | Zinc 3 | H | ||||||||
METAL | 103 | 103 | Zinc 3 | H | ||||||||
METAL | 116 | 116 | Zinc 1 | C | ||||||||
METAL | 119 | 119 | Zinc 1 | C |
Size range | 812-901 amino acids |
Related rules | None |
Fusion | None |