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Annotation rule MF_03062
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General rule information [?]

Accession MF_03062
Dates 26-FEB-2009 (Created)
16-MAR-2021 (Last updated, Version 16)
Name UBP16
Scope
Eukaryota; Vertebrata
Template Q9Y5T5 (UBP16_HUMAN)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
UBP16
Protein name
RecName: Full=Ubiquitin carboxyl-terminal hydrolase 16;
EC 3.4.19.12;
AltName: Full=Deubiquitinating enzyme 16;
AltName: Full=Ubiquitin thioesterase 16;
AltName: Full=Ubiquitin-specific-processing protease 16;
Gene name
USP16

Comments [?]

case <OC:Mammalia>
Function Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.
else
Function Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.
end case
Catalytic activity Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12;
Subunit Homotetramer.
Subcellular location Nucleus.
Domain The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.
case <OC:Mammalia>
Ptm Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.
end case
Similarity Belongs to the peptidase C19 family. USP16 subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005634; Cellular component: nucleus.
GO:0004197; Molecular function: cysteine-type endopeptidase activity.
GO:0042393; Molecular function: histone binding.
GO:0003713; Molecular function: transcription coactivator activity.
GO:0043130; Molecular function: ubiquitin binding.
GO:0004843; Molecular function: thiol-dependent deubiquitinase.
GO:0008270; Molecular function: zinc ion binding.
GO:0000278; Biological process: mitotic cell cycle.
GO:0016578; Biological process: histone deubiquitination.
GO:0140014; Biological process: mitotic nuclear division.
GO:0045893; Biological process: positive regulation of transcription, DNA-templated.
GO:0051289; Biological process: protein homotetramerization.

Cross-references [?]

PROSITE PS00972; USP_1; 1;
PS00973; USP_2; 1;
PS50235; USP_3; 1;
PS50271; ZF_UBP; 1; trigger=PRU00502;
Pfam PF00443; UCH; 1;
PF02148; zf-UBP; 1;

Features [?]

From: UBP16_HUMAN (Q9Y5T5)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     205     205       Nucleophile     C  
ACT_SITE     758     758       Proton acceptor     H  
METAL     24     24       Zinc 1     C  
METAL     26     26       Zinc 1     H  
METAL     48     48       Zinc 2     C  
METAL     51     51       Zinc 2     C  
METAL     74     74       Zinc 3     C  
METAL     77     77       Zinc 3     C  
METAL     82     82       Zinc 2     C  
METAL     90     90       Zinc 2     H  
METAL     94     94       Zinc 3     H  
METAL     103     103       Zinc 3     H  
METAL     116     116       Zinc 1     C  
METAL     119     119       Zinc 1     C  

Additional information [?]

Size range 812-901 amino acids
Related rules None
Fusion None