HAMAP

Accession	MF_03062
Dates	26-FEB-2009 (Created) 16-MAR-2021 (Last updated, Version 16)

Name

UBP16

Scope

Eukaryota; Vertebrata

Template

Q9Y5T5 (UBP16_HUMAN)

Triggered by

HAMAP; MF_03062 (Get profile general information and statistics)

Identifier

UBP16

Protein name

RecName: Full=Ubiquitin carboxyl-terminal hydrolase 16; EC 3.4.19.12; AltName: Full=Deubiquitinating enzyme 16; AltName: Full=Ubiquitin thioesterase 16; AltName: Full=Ubiquitin-specific-processing protease 16;

Gene name

USP16

Function

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.

Function

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.

Catalytic activity	Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12;
Subunit	Homotetramer.
Subcellular location	Nucleus.
Domain	The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.

Ptm	Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.

Similarity

Belongs to the peptidase C19 family. USP16 subfamily.

Activator
Cell cycle
Cell division
Chromatin regulator
Hydrolase
Metal-binding
Mitosis
Nucleus
Protease
Thiol protease
Transcription
Transcription regulation
Ubl conjugation pathway
Zinc
Zinc-finger

GO:0005634; Cellular component: nucleus.
GO:0004197; Molecular function: cysteine-type endopeptidase activity.
GO:0042393; Molecular function: histone binding.
GO:0003713; Molecular function: transcription coactivator activity.
GO:0043130; Molecular function: ubiquitin binding.
GO:0004843; Molecular function: thiol-dependent deubiquitinase.
GO:0008270; Molecular function: zinc ion binding.
GO:0000278; Biological process: mitotic cell cycle.
GO:0016578; Biological process: histone deubiquitination.
GO:0140014; Biological process: mitotic nuclear division.
GO:0045893; Biological process: positive regulation of transcription, DNA-templated.
GO:0051289; Biological process: protein homotetramerization.

PROSITE	PS00972; USP_1; 1;
	PS00973; USP_2; 1;
	PS50235; USP_3; 1;
	PS50271; ZF_UBP; 1; trigger=PRU00502;
Pfam	PF00443; UCH; 1;
	PF02148; zf-UBP; 1;

From: UBP16_HUMAN (Q9Y5T5)

Key

From

To

Description

Tag

Condition

FTGroup

ACT_SITE

205

205

Nucleophile

C

ACT_SITE

758

758

Proton acceptor

H

METAL

24

24

Zinc 1

C

METAL

26

26

Zinc 1

H

METAL

48

48

Zinc 2

C

METAL

51

51

Zinc 2

C

METAL

74

74

Zinc 3

C

METAL

77

77

Zinc 3

C

METAL

82

82

Zinc 2

C

METAL

90

90

Zinc 2

H

METAL

94

94

Zinc 3

H

METAL

103

103

Zinc 3

H

METAL

116

116

Zinc 1

C

METAL

119

119

Zinc 1

C

Size range	812-901 amino acids
Related rules	None
Fusion	None

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