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Annotation rule MF_03106 |
General rule information
[?]
Accession |
MF_03106 |
Dates |
14-AUG-2009 (Created) 20-NOV-2019 (Last updated, Version 7) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
case <OC:Saccharomyces>
Protein name |
RecName:
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Full=Sulfate adenylyltransferase;
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EC 2.7.7.4;
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AltName:
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Full=ATP-sulfurylase;
|
AltName:
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Full=Methionine-requiring protein 3;
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AltName:
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Full=Sulfate adenylate transferase;
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Short=SAT;
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|
else
Protein name |
RecName:
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Full=Sulfate adenylyltransferase;
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EC 2.7.7.4;
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AltName:
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Full=ATP-sulfurylase;
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AltName:
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Full=Sulfate adenylate transferase;
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Short=SAT;
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|
end case
Function |
Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids. |
Catalytic activity |
RHEA:18133: ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate
EC 2.7.7.4
|
Pathway |
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. |
Subunit |
Homohexamer. Dimer of trimers. |
Subcellular location |
Cytoplasm. |
case <IPRO:IPR002891>
Activity regulation |
Allosterically inhibited by 3'-phosphoadenosine 5'-phosphosulfate (PAPS). |
Domain |
The adenylyl-sulfate kinase (APS kinase) is non-functional. It is involved in allosteric regulation by PAPS. PAPS binding induces a large rotational rearrangement of domains lowering the substrate affinity of the enzyme. |
Similarity |
In the N-terminal section; belongs to the sulfate adenylyltransferase family. |
|
In the C-terminal section; belongs to the APS kinase family. |
else
Domain |
The oligomerization domain is distantly related to APS kinases, but it is not functional and does not bind APS. It is required for oligomerization of the enzyme, although the oligomerization state has no effect on the catalytic activity of the enzyme. |
Similarity |
Belongs to the sulfate adenylyltransferase family. |
end case
case <IPRO:IPR002891>
end case
case <OC:Saccharomyces>
end case
GO:0004781; Molecular function: sulfate adenylyltransferase (ATP) activity.
GO:0000103; Biological process: sulfate assimilation.
GO:0005737; Cellular component: cytoplasm.
From: MET3_YEAST (P08536) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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NP_BIND
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195
|
|
198
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ATP
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|
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Q-T-R-N
|
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NP_BIND
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289
|
|
292
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|
ATP
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|
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G-R-D-H
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|
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ACT_SITE
|
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196
|
|
196
|
|
|
|
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T
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|
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ACT_SITE
|
|
197
|
|
197
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|
|
|
|
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R
|
|
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ACT_SITE
|
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198
|
|
198
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N
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BINDING
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195
|
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195
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Sulfate
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Q
|
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BINDING
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197
|
|
197
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Sulfate
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|
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R
|
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BINDING
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293
|
|
293
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Sulfate; via amide nitrogen
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A
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BINDING
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331
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331
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ATP; via amide nitrogen
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[MILV]
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SITE
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201
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201
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Transition state stabilizer
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H
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SITE
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204
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204
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Transition state stabilizer
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H
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SITE
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328
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328
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Induces change in substrate recognition on ATP binding
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F
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REGION
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Nter
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167
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N-terminal
|
|
|
|
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REGION
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168
|
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393
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Catalytic
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case not <IPRO:IPR002891>
REGION
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394
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Cter
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Required for oligomerization; adenylyl-sulfate kinase-like
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else
From: MET3_PENCH (Q12650) |
REGION
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394
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Cter
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Allosteric regulation domain; adenylyl-sulfate kinase-like
|
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REGION
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433
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436
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Allosteric inhibitor PAPS binding
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[DE]-x(2)-R
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REGION
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476
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477
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Allosteric inhibitor PAPS binding
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[IT]-A
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BINDING
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450
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450
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Allosteric inhibitor PAPS
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R
|
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BINDING
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514
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514
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Allosteric inhibitor PAPS
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[RK]
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end case
Additional information
[?]
Size range |
490-581 amino acids |
Related rules |
None |
Fusion |
None |