HAMAP logo

HAMAP rule MF_03112

Send feedback

General rule information [?]

Accession MF_03112
Dates 8-OCT-2009 (Created)
1-JUN-2023 (Last updated, Version 9)
Name Asna1_Get3
Scope
Eukaryota
Template Q12154 (GET3_YEAST)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Fungi>
Identifier
GET3
else
Identifier
ASNA
end case
case <OC:Fungi>
Protein name
RecName: Full=ATPase ;
EC 3.6.-.-;
AltName: Full=Arsenical pump-driving ATPase;
AltName: Full=Arsenite-stimulated ATPase;
AltName: Full=Golgi to ER traffic protein 3;
AltName: Full=Guided entry of tail-anchored proteins 3;
else case <OC:Vertebrata> or <OC:Caenorhabditis> or <OC:Viridiplantae>
Protein name
RecName: Full=ATPase ;
EC 3.6.-.-;
AltName: Full=Arsenical pump-driving ATPase;
AltName: Full=Arsenite-stimulated ATPase;
else
Protein name
RecName: Full=ATPase ASNA1 homolog;
EC 3.6.-.-;
AltName: Full=Arsenical pump-driving ATPase homolog;
AltName: Full=Arsenite-stimulated ATPase;
end case
case <OC:Fungi>
Gene name
GET3
else case <OC:Vertebrata>
Gene name
ASNA1
else case <OC:Caenorhabditis>
Gene name
asna-1
end case

Comments [?]

case <OC:Saccharomycotina>
Function ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors @gn(GET1) and @gn(GET2), where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the @gn(GET1)-@gn(GET2) receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.
else
Function ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.
end case
case <OC:Saccharomycotina>
Subunit Homodimer. Component of the Golgi to ER traffic (GET) complex, which is composed of @gn(GET1), @gn(GET2) and @gn(GET3). Within the complex, @gn(GET1) and @gn(GET2) form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the @gn(GET3) homodimer. Interacts with the chloride channel protein GEF1.
Subcellular location Cytoplasm. Endoplasmic reticulum. Golgi apparatus. Note=@gn(GET1) and @gn(GET2) are required for targeting @gn(GET3) to the endoplasmic reticulum.
else case <OC:Mammalia>
Subunit Homodimer. Component of a transmembrane domain recognition complex (TRC). Interacts with @gn(SERP1) and @gn(SEC61B). Interacts with @gn(WRB).
Subcellular location Cytoplasm. Endoplasmic reticulum. Nucleus, nucleolus.
else
Subunit Homodimer.
Subcellular location Cytoplasm. Endoplasmic reticulum.
end case
Similarity Belongs to the arsA ATPase family.

Keywords [?]

Cytoplasm
Endoplasmic reticulum
Hydrolase
Transport
case <FTGroup:1>
ATP-binding
Nucleotide-binding
end case
case <FTGroup:2>
Metal-binding
Zinc
end case
case <OC:Saccharomyces>
Arsenical resistance
ER-Golgi transport
Golgi apparatus
end case
case <OC:Mammalia>
Nucleus
end case

Gene Ontology [?]

case <OC:Saccharomyces>
GO:0043529; Cellular component: GET complex.
else
GO:0005783; Cellular component: endoplasmic reticulum.
end case
GO:0045048; Biological process: protein insertion into ER membrane.
GO:0005524; Molecular function: ATP binding.

Cross-references [?]

Pfam PF02374; ArsA_ATPase; 1;
NCBIfam TIGR00345; arsA; 1;

Features [?]

From: GET3_YEAST (Q12154)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     26     33       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     K-G-G-V-G-K-T-T   1
ACT_SITE     57     57             D  
BINDING     285     285       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="ligand shared between dimeric partners     C   2
BINDING     288     288       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="ligand shared between dimeric partners     C   2
BINDING     245     245       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     E   1
BINDING     272     272       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     N   1

Additional information [?]

Size range 324-409 amino acids
Related rules None
Fusion None


View rule in raw text format (no links)