HAMAP rule MF_03112
General rule information
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Accession | MF_03112 |
Dates | 8-OCT-2009 (Created) 1-JUN-2023 (Last updated, Version 9) |
Name | Asna1_Get3 |
Scope | Eukaryota |
Template | Q12154 (GET3_YEAST) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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case <OC:Fungi>
Identifier |
|
else
Identifier |
|
end case
case <OC:Fungi>
Protein name |
|
else case <OC:Vertebrata> or <OC:Caenorhabditis> or <OC:Viridiplantae>
Protein name |
|
else
Protein name |
|
end case
case <OC:Fungi>
Gene name |
|
else case <OC:Vertebrata>
Gene name |
|
else case <OC:Caenorhabditis>
Gene name |
|
end case
Comments
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case <OC:Saccharomycotina>
Function | ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors @gn(GET1) and @gn(GET2), where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the @gn(GET1)-@gn(GET2) receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance. |
else
Function | ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting. |
end case
case <OC:Saccharomycotina>
Subunit | Homodimer. Component of the Golgi to ER traffic (GET) complex, which is composed of @gn(GET1), @gn(GET2) and @gn(GET3). Within the complex, @gn(GET1) and @gn(GET2) form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the @gn(GET3) homodimer. Interacts with the chloride channel protein GEF1. |
Subcellular location | Cytoplasm. Endoplasmic reticulum. Golgi apparatus. Note=@gn(GET1) and @gn(GET2) are required for targeting @gn(GET3) to the endoplasmic reticulum. |
else case <OC:Mammalia>
Subunit | Homodimer. Component of a transmembrane domain recognition complex (TRC). Interacts with @gn(SERP1) and @gn(SEC61B). Interacts with @gn(WRB). |
Subcellular location | Cytoplasm. Endoplasmic reticulum. Nucleus, nucleolus. |
else
Subunit | Homodimer. |
Subcellular location | Cytoplasm. Endoplasmic reticulum. |
end case
Similarity | Belongs to the arsA ATPase family. |
Keywords
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case <FTGroup:1>
end case
case <FTGroup:2>
end case
case <OC:Saccharomyces>
end case
case <OC:Mammalia>
end case
Gene Ontology
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case <OC:Saccharomyces>
GO:0043529; Cellular component: GET complex.
else
GO:0005783; Cellular component: endoplasmic reticulum.
end case
GO:0045048; Biological process: protein insertion into ER membrane.
GO:0005524; Molecular function: ATP binding.
GO:0005524; Molecular function: ATP binding.
Cross-references
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Features
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From: GET3_YEAST (Q12154) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 26 | 33 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | K-G-G-V-G-K-T-T | 1 | |||||||
ACT_SITE | 57 | 57 | D | |||||||||
BINDING | 285 | 285 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="ligand shared between dimeric partners | C | 2 | |||||||
BINDING | 288 | 288 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="ligand shared between dimeric partners | C | 2 | |||||||
BINDING | 245 | 245 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | E | 1 | |||||||
BINDING | 272 | 272 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | N | 1 |
Additional information
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Size range | 324-409 amino acids |
Related rules | None |
Fusion | None |