HAMAP rule MF_03112

General rule information [?]

Accession	MF_03112
Dates	8-OCT-2009 (Created) 1-JUN-2023 (Last updated, Version 9)
Name	Asna1_Get3
Scope(s)	Eukaryota
Template(s)	Q12154 (GET3_YEAST); [ Recover all ]
Triggered by	HAMAP; MF_03112 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Fungi>
Identifier	GET3
else
Identifier	ASNA
end case
case <OC:Fungi>
Protein name	RecName: Full=ATPase ; EC=3.6.-.-; AltName: Full=Arsenical pump-driving ATPase; AltName: Full=Arsenite-stimulated ATPase; AltName: Full=Golgi to ER traffic protein 3; AltName: Full=Guided entry of tail-anchored proteins 3;
else case <OC:Vertebrata> or <OC:Caenorhabditis> or <OC:Viridiplantae>
Protein name	RecName: Full=ATPase ; EC=3.6.-.-; AltName: Full=Arsenical pump-driving ATPase; AltName: Full=Arsenite-stimulated ATPase;
else
Protein name	RecName: Full=ATPase ASNA1 homolog; EC=3.6.-.-; AltName: Full=Arsenical pump-driving ATPase homolog; AltName: Full=Arsenite-stimulated ATPase;
end case
case <OC:Fungi>
Gene name	Name=GET3;
else case <OC:Vertebrata>
Gene name	Name=ASNA1;
else case <OC:Caenorhabditis>
Gene name	Name=asna-1;
end case

Comments [?]

case <OC:Saccharomycotina>
FUNCTION	ATPase required for the post-translational delivery of tail- anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors @gn(GET1) and @gn(GET2), where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the @gn(GET1)-@gn(GET2) receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.
else
FUNCTION	ATPase required for the post-translational delivery of tail- anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.
end case
case <OC:Saccharomycotina>
SUBUNIT	Homodimer. Component of the Golgi to ER traffic (GET) complex, which is composed of @gn(GET1), @gn(GET2) and @gn(GET3). Within the complex, @gn(GET1) and @gn(GET2) form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the @gn(GET3) homodimer. Interacts with the chloride channel protein GEF1.
SUBCELLULAR LOCATION	Cytoplasm. Endoplasmic reticulum. Golgi apparatus. Note=@gn(GET1) and @gn(GET2) are required for targeting @gn(GET3) to the endoplasmic reticulum.
else case <OC:Mammalia>
SUBUNIT	Homodimer. Component of a transmembrane domain recognition complex (TRC). Interacts with @gn(SERP1) and @gn(SEC61B). Interacts with @gn(WRB).
SUBCELLULAR LOCATION	Cytoplasm. Endoplasmic reticulum. Nucleus, nucleolus.
else
SUBUNIT	Homodimer.
SUBCELLULAR LOCATION	Cytoplasm. Endoplasmic reticulum.
end case
SIMILARITY	Belongs to the arsA ATPase family.

Keywords [?]

Cytoplasm
Endoplasmic reticulum
Hydrolase
Transport
case <FTGroup:1>
ATP-binding
Nucleotide-binding
end case
case <FTGroup:2>
Metal-binding
Zinc
end case
case <OC:Saccharomyces>
Arsenical resistance
ER-Golgi transport
Golgi apparatus
end case
case <OC:Mammalia>
Nucleus
end case

Gene Ontology [?]

case <OCellular component:Saccharomyces>
GO:0043529; Cellular component:GET complex
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0005783; Cellular component:endoplasmic reticulum
end case
GO:0045048; Biological process:protein insertion into ER membrane
GO:0005524; Molecular function:ATP binding

Cross-references [?]

Pfam	PF02374; ArsA_ATPase; 1;
NCBIfam	TIGR00345; arsA; 1;

Features [?]

From: GET3_YEAST (Q12154)
Key	From	To	Description	Tag	Condition	FTGroup
BINDING	26	33	/ligand="ATP" /ligand_id="ChEBI:CHEBI:30616"		K-G-G-V-G-K-T-T	1
ACT_SITE	57	57			D
BINDING	285	285	/ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="ligand shared between dimeric partners"		C	2
BINDING	288	288	/ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="ligand shared between dimeric partners"		C	2
BINDING	245	245	/ligand="ATP" /ligand_id="ChEBI:CHEBI:30616"		E	1
BINDING	272	272	/ligand="ATP" /ligand_id="ChEBI:CHEBI:30616"		N	1

Additional information [?]

Size range	324-409 amino acids
Related rules	None
Fusion	Nter: None Cter: None

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