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Annotation rule MF_03112 |
General rule information
[?]
Accession |
MF_03112 |
Dates |
8-OCT-2009 (Created) 20-NOV-2019 (Last updated, Version 5) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
case <OC:Fungi>
else
end case
case <OC:Fungi>
Protein name |
RecName:
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Full=ATPase ;
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EC 3.6.-.-;
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AltName:
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Full=Arsenical pump-driving ATPase;
|
AltName:
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Full=Arsenite-stimulated ATPase;
|
AltName:
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Full=Golgi to ER traffic protein 3;
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AltName:
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Full=Guided entry of tail-anchored proteins 3;
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|
else case <OC:Vertebrata> or <OC:Caenorhabditis> or <OC:Viridiplantae>
Protein name |
RecName:
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Full=ATPase ;
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EC 3.6.-.-;
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AltName:
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Full=Arsenical pump-driving ATPase;
|
AltName:
|
Full=Arsenite-stimulated ATPase;
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|
else
Protein name |
RecName:
|
Full=ATPase ASNA1 homolog;
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EC 3.6.-.-;
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AltName:
|
Full=Arsenical pump-driving ATPase homolog;
|
AltName:
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Full=Arsenite-stimulated ATPase;
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|
end case
case <OC:Fungi>
else case <OC:Vertebrata>
else case <OC:Caenorhabditis>
end case
case <OC:Saccharomyces>
Function |
ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance. |
else
Function |
ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting. |
end case
case <OC:Saccharomyces>
Subunit |
Homodimer. Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Interacts with the chloride channel protein GEF1. |
Subcellular location |
Cytoplasm. Endoplasmic reticulum. Golgi apparatus. Note=GET1 and GET2 are required for targeting GET3 to the endoplasmic reticulum. |
else case <OC:Mammalia>
Subunit |
Homodimer. Component of a transmembrane domain recognition complex (TRC). Interacts with @gn(SERP1) and @gn(SEC61B). Interacts with @gn(WRB). |
Subcellular location |
Cytoplasm. Endoplasmic reticulum. Nucleus, nucleolus. |
else
Subunit |
Homodimer. |
Subcellular location |
Cytoplasm. Endoplasmic reticulum. |
end case
Similarity |
Belongs to the arsA ATPase family. |
case <FTGroup:1>
end case
case <FTGroup:2>
end case
case <OC:Saccharomyces>
end case
case <OC:Mammalia>
end case
case <OC:Saccharomyces>
else
GO:0005783; Cellular component: endoplasmic reticulum.
end case
GO:0045048; Biological process: protein insertion into ER membrane.
GO:0005524; Molecular function: ATP binding.
From: GET3_YEAST (Q12154) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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NP_BIND
|
|
26
|
|
33
|
|
ATP
|
|
|
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K-G-G-V-G-K-T-T
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|
1
|
ACT_SITE
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57
|
|
57
|
|
|
|
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|
D
|
|
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METAL
|
|
285
|
|
285
|
|
Zinc; shared with dimeric partner
|
|
|
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C
|
|
2
|
METAL
|
|
288
|
|
288
|
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Zinc; shared with dimeric partner
|
|
|
|
C
|
|
2
|
BINDING
|
|
245
|
|
245
|
|
ATP
|
|
|
|
E
|
|
1
|
BINDING
|
|
272
|
|
272
|
|
ATP
|
|
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N
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1
|
Additional information
[?]
Size range |
324-409 amino acids |
Related rules |
None |
Fusion |
None |