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HAMAP rule MF_03117

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General rule information [?]

Accession MF_03117
Dates 1-APR-2010 (Created)
1-JUN-2023 (Last updated, Version 12)
Name Salvage_MtnC_euk
Scope(s) Eukaryota
Template(s) Q9UHY7 (ENOPH_HUMAN); P32626 (ENOPH_YEAST); [ Recover all ]
Triggered by HAMAP; MF_03117 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ENOPH
case <OC:Vertebrata>
Protein name RecName: Full=Enolase-phosphatase E1;
                 EC=3.1.3.77;
AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase;
AltName: Full=MASA homolog;
else case <OC:Saccharomyces>
Protein name RecName: Full=Enolase-phosphatase E1;
                 EC=3.1.3.77;
AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase;
AltName: Full=Unknown transcript 4 protein;
else
Protein name RecName: Full=Enolase-phosphatase E1;
                 EC=3.1.3.77;
AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase;
end case
case <OC:Vertebrata>
Gene name Name=ENOPH1; Synonyms=MASA;
else case <OC:Fungi>
Gene name Name=UTR4;
end case

Comments [?]

FUNCTIONBifunctional enzyme that catalyzes the enolization of 2,3- diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK- MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
CATALYTIC ACTIVITY Reaction=5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2- dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate; Xref=Rhea:RHEA:21700, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:49252, ChEBI:CHEBI:58828; EC=3.1.3.77;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
PATHWAYAmino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.
PATHWAYAmino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
SUBUNITMonomer.
SUBCELLULAR LOCATIONCytoplasm. Nucleus.
SIMILARITYBelongs to the HAD-like hydrolase superfamily. MasA/MtnC family.

Keywords [?]


Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0043715; Molecular function:2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity
GO:0043716; Molecular function:2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity
GO:0019284; Biological process:L-methionine salvage from S-adenosylmethionine

Cross-references [?]

Pfam PF00702; Hydrolase; 1;
PRINTS PR00413; HADHALOGNASE; 1;
NCBIfam TIGR01691; enolase-ppase; 1;
NCBIfam TIGR01549; HAD-SF-IA-v1; 1;

Features [?]

From: ENOPH_HUMAN (Q9UHY7)
Key From To Description Tag Condition FTGroup
BINDING 153 154 /ligand="substrate" S-S
BINDING 16 16 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D 1
BINDING 18 18 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
E 1
BINDING 212 212 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D 1
BINDING 187 187 /ligand="substrate" K

Additional information [?]

Size range 215-1107 amino acids
Related rules MF_03118
Fusion Nter: None Cter: None



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