HAMAP logo

HAMAP rule MF_03117

Send feedback

General rule information [?]

Accession MF_03117
Dates 1-APR-2010 (Created)
1-JUN-2023 (Last updated, Version 12)
Name Salvage_MtnC_euk
Scope
Eukaryota
Templates Q9UHY7 (ENOPH_HUMAN); P32626 (ENOPH_YEAST): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ENOPH
case <OC:Vertebrata>
Protein name
RecName: Full=Enolase-phosphatase E1;
EC 3.1.3.77;
AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase;
AltName: Full=MASA homolog;
else case <OC:Saccharomyces>
Protein name
RecName: Full=Enolase-phosphatase E1;
EC 3.1.3.77;
AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase;
AltName: Full=Unknown transcript 4 protein;
else
Protein name
RecName: Full=Enolase-phosphatase E1;
EC 3.1.3.77;
AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase;
end case
case <OC:Vertebrata>
Gene name
ENOPH1, MASA
else case <OC:Fungi>
Gene name
UTR4
end case

Comments [?]

Function Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
Catalytic activity RHEA:21700: 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate
EC 3.1.3.77
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
Pathway Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit Monomer.
Subcellular location Cytoplasm. Nucleus.
Similarity Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.

Keywords [?]

Amino-acid biosynthesis
Cytoplasm
Hydrolase
case <FTGroup:1>
Magnesium
Metal-binding
end case
Methionine biosynthesis
Nucleus

Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0043715; Molecular function: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity.
GO:0043716; Molecular function: 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity.
GO:0019284; Biological process: L-methionine salvage from S-adenosylmethionine.

Cross-references [?]

Pfam PF00702; Hydrolase; 1;
PRINTS PR00413; HADHALOGNASE; 1;
NCBIfam TIGR01691; enolase-ppase; 1;
TIGR01549; HAD-SF-IA-v1; 1;

Features [?]

From: ENOPH_HUMAN (Q9UHY7)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     153     154       /ligand="substrate     S-S  
BINDING     16     16       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D   1
BINDING     18     18       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     E   1
BINDING     212     212       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D   1
BINDING     187     187       /ligand="substrate     K  

Additional information [?]

Size range 215-1107 amino acids
Related rules MF_03118 (MTBC supersedes the current rule)
Fusion None


View rule in raw text format (no links)