HAMAP rule MF_03123
General rule information
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| Accession | MF_03123 |
| Dates | 23-JUL-2010 (Created)
14-SEP-2023 (Last updated, Version 17) |
| Name | Lipoyl_synth_euk |
| Scope(s) |
Eukaryota Plastid |
| Template(s) | P32875 (LIPA_YEAST); Q8IDQ0 (LIPA_PLAF7); [ Recover all ] |
| Triggered by |
case c? <OC:Eukaryota> or <OG:Plastid>
HAMAP; MF_00206 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Metazoa> | |
| Identifier | LIAS |
| else | |
| Identifier | LIPA |
| end case | |
| case <OG:Organellar chromatophore> | |
| Protein name | RecName: Full=Lipoyl synthase, organellar chromatophore; EC=2.8.1.8; AltName: Full=Lipoate synthase; Short=Lip-syn; Short=LS; AltName: Full=Lipoic acid synthase; |
| else case <OC:Apicomplexa> | |
| Protein name | RecName: Full=Lipoyl synthase, apicoplast; EC=2.8.1.8; AltName: Full=Lipoate synthase; Short=Lip-syn; Short=LS; AltName: Full=Lipoic acid synthase; |
| else | |
| Protein name | RecName: Full=Lipoyl synthase, mitochondrial; EC=2.8.1.8; AltName: Full=Lipoate synthase; Short=Lip-syn; Short=LS; AltName: Full=Lipoic acid synthase; |
| end case | |
| case <OC:Vertebrata> | |
| Gene name | Name=LIAS; |
| else case <OC:Drosophilidae> | |
| Gene name | Name=Las; |
| else case <OC:Apicomplexa> | |
| Gene name | Name=lipA; |
| else case <OC:Saccharomyces> | |
| Gene name | Name=LIP5; |
| end case | |
Comments
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| FUNCTION | Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. |
| CATALYTIC ACTIVITY | Reaction=[[Fe-S] cluster scaffold protein carrying a second [4Fe- 4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'- deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + N(6)-[(R)-dihydrolipoyl]-L-lysyl- [protein] + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:16585, Rhea:RHEA-COMP:9928, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:10475, Rhea:RHEA-COMP:14568, Rhea:RHEA-COMP:14569, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:29034, ChEBI:CHEBI:29919, ChEBI:CHEBI:33722, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:78809, ChEBI:CHEBI:83100; EC=2.8.1.8; |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
| PATHWAY | Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2. |
| case <OG:Organellar chromatophore> | |
| SUBCELLULAR LOCATION | Plastid, organellar chromatophore. |
| else case <OC:Apicomplexa> | |
| SUBCELLULAR LOCATION | Plastid, apicoplast. |
| else | |
| SUBCELLULAR LOCATION | Mitochondrion. |
| end case | |
| case not <OC:Apicomplexa> and not <OG:Organellar chromatophore> and not <AnyFeature:TransitM> | |
| MISCELLANEOUS | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
| end case | |
| SIMILARITY | Belongs to the radical SAM superfamily. Lipoyl synthase family. |
Keywords
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| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| case <OG:Organellar chromatophore> | |
| Organellar chromatophore | |
| Plastid | |
| else case <OC:Apicomplexa> | |
| Apicoplast | |
| Plastid | |
| Signal | |
| else | |
| Mitochondrion | |
| end case | |
| S-adenosyl-L-methionine | |
| Transferase | |
Gene Ontology
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| GO:0016783; Molecular function:sulfurtransferase activity | |
| GO:0016992; Molecular function:lipoate synthase activity | |
| GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding | |
| GO:0009107; Biological process:lipoate biosynthetic process | |
| GO:0009249; Biological process:protein lipoylation | |
| case <OG:Organellar chromatophore> | |
| GO:0070111; Cellular component:organellar chromatophore | |
| else case <OCellular component:Apicomplexa> | |
| GO:0020011; Cellular component:apicoplast | |
| else; https://www.ebi.ac.uk/QuickGO/term/else | |
| GO:0005739; Cellular component:mitochondrion | |
| end case | |
Cross-references
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| Pfam | PF04055; Radical_SAM; 1; |
| NCBIfam | TIGR00510; lipA; 1; |
| PIRSF | PIRSF005963; Lipoyl_synth; 1; |
| General | Signal; -; 1; |
| General | TransitM; -; 0-1; |
Features
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| From: LIPA_YEAST (P32875) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 150 | 150 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 155 | 155 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 161 | 161 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 181 | 181 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
| BINDING | 185 | 185 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
| BINDING | 188 | 188 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
| BINDING | 396 | 396 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
S | ||||||||
Additional information
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| Size range | 291-502 amino acids |
| Related rules |
MF_03128 MF_03129 |
| Fusion | Nter: None Cter: None |