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Annotation rule MF_03123
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General rule information [?]

Accession MF_03123
Dates 23-JUL-2010 (Created)
20-NOV-2019 (Last updated, Version 12)
Name Lipoyl_synth_euk
Scope
Eukaryota; except Viridiplantae
Plastid
Templates P32875 (LIPA_YEAST); Q8IDQ0 (LIPA_PLAF7): [Recover all]
case <OC:Eukaryota> or <OG:Plastid>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Metazoa>
Identifier
LIAS
else
Identifier
LIPA
end case
case <OG:Organellar chromatophore>
Protein name
RecName: Full=Lipoyl synthase, organellar chromatophore;
EC 2.8.1.8;
AltName: Full=Lipoate synthase;
Short=Lip-syn;
Short=LS;
AltName: Full=Lipoic acid synthase;
else case <OC:Apicomplexa>
Protein name
RecName: Full=Lipoyl synthase, apicoplast;
EC 2.8.1.8;
AltName: Full=Lipoate synthase;
Short=Lip-syn;
Short=LS;
AltName: Full=Lipoic acid synthase;
else
Protein name
RecName: Full=Lipoyl synthase, mitochondrial;
EC 2.8.1.8;
AltName: Full=Lipoate synthase;
Short=Lip-syn;
Short=LS;
AltName: Full=Lipoic acid synthase;
end case
case <OC:Vertebrata>
Gene name
LIAS
else case <OC:Drosophilidae>
Gene name
Las
else case <OC:Apicomplexa>
Gene name
lipA
else case <OC:Saccharomyces>
Gene name
LIP5
end case

Comments [?]

Function Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.
Catalytic activity RHEA:16585: [[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]
EC 2.8.1.8
Cofactor [4Fe-4S] cluster
Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.
case <OG:Organellar chromatophore>
Subcellular location Plastid, organellar chromatophore.
else case <OC:Apicomplexa>
Subcellular location Plastid, apicoplast.
else
Subcellular location Mitochondrion.
end case
case not <OC:Apicomplexa> and not <OG:Organellar chromatophore> and not <AnyFeature:TransitM>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
Similarity Belongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords [?]

case <OG:Organellar chromatophore>
else case <OC:Apicomplexa>
else
end case

Gene Ontology [?]

GO:0016783; Molecular function: sulfurtransferase activity.
GO:0016992; Molecular function: lipoate synthase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0009107; Biological process: lipoate biosynthetic process.
GO:0009249; Biological process: protein lipoylation.
case <OG:Organellar chromatophore>
GO:0070111; Cellular component: organellar chromatophore.
else case <OC:Apicomplexa>
GO:0020011; Cellular component: apicoplast.
else
GO:0005739; Cellular component: mitochondrion.
end case

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
TIGRFAMs TIGR00510; lipA; 1;
PIRSF PIRSF005963; Lipoyl_synth; 1;

Computed features [?]

case <OC:Apicomplexa>
General Signal; -; 1; trigger=yes;
else case not <OG:Organellar chromatophore>
TransitM; -; 0-1; trigger=yes;
end case

Features [?]

From: LIPA_YEAST (P32875)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     150     150       Iron-sulfur 1 (4Fe-4S)     C  
METAL     155     155       Iron-sulfur 1 (4Fe-4S)     C  
METAL     161     161       Iron-sulfur 1 (4Fe-4S)     C  
METAL     181     181       Iron-sulfur 2 (4Fe-4S-S-AdoMet)     C  
METAL     185     185       Iron-sulfur 2 (4Fe-4S-S-AdoMet)     C  
METAL     188     188       Iron-sulfur 2 (4Fe-4S-S-AdoMet)     C  
METAL     396     396       Iron-sulfur 1 (4Fe-4S)     S  

Additional information [?]

Size range 291-502 amino acids
Related rules MF_03128 (LIAS supersedes the current rule); MF_03129 (LISC supersedes the current rule)
Fusion None