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Annotation rule MF_03126
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General rule information [?]

Accession MF_03126
Dates 13-AUG-2010 (Created)
20-NOV-2019 (Last updated, Version 6)
Name Adenylosucc_synth_vert_basic
Scope
Eukaryota; Vertebrata
Templates P28650 (PURA1_MOUSE); P0A7D4 (PURA_ECOLI): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PURA1
Protein name
RecName: Full=Adenylosuccinate synthetase isozyme 1;
Short=AMPSase 1;
Short=AdSS 1;
EC 6.3.4.4;
AltName: Full=Adenylosuccinate synthetase, basic isozyme;
AltName: Full=Adenylosuccinate synthetase, muscle isozyme;
Short=M-type adenylosuccinate synthetase;
AltName: Full=IMP--aspartate ligase 1;
Gene name
ADSSL1, ADSS1

Comments [?]

Function Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
Catalytic activity RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate
EC 6.3.4.4
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
Pathway Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the adenylosuccinate synthetase family.

Keywords [?]

Cytoplasm
GTP-binding
Ligase
Magnesium
Metal-binding
Nucleotide-binding
Purine biosynthesis

Gene Ontology [?]

GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS01266; ADENYLOSUCCIN_SYN_1; 1;
PS00513; ADENYLOSUCCIN_SYN_2; 1;
Pfam PF00709; Adenylsucc_synt; 1;
TIGRFAMs TIGR00184; PurA; 1;

Features [?]

From: PURA1_MOUSE (P28650)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     42     48       GTP     G-D-E-G-K-G-K  
NP_BIND     70     72       GTP     G-H-T  
NP_BIND     363     365       GTP     K-L-D  
NP_BIND     445     448       GTP     G-V-G-K  
REGION     43     46       IMP binding     D-E-G-K  
REGION     68     71       IMP binding     N-A-G-H  
REGION     331     337       Substrate binding     x-x-T-x-[KR]-x-R  
ACT_SITE     43     43       Proton acceptor     D  
ACT_SITE     71     71       Proton donor     H  
METAL     43     43       Magnesium     D  
METAL     70     70       Magnesium; via carbonyl oxygen     G  
BINDING     43     43       Substrate     D  
BINDING     163     163       IMP     T  
BINDING     177     177       IMP; shared with dimeric partner     R  
BINDING     256     256       IMP     N  
BINDING     271     271       IMP     T  
BINDING     335     335       IMP     R  
BINDING     337     337       GTP     R  

Additional information [?]

Size range 449-459 amino acids
Related rules MF_03125 (PURA); MF_03127 (PURA2)
Fusion None


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