HAMAP rule MF_03126

General rule information [?]

Accession	MF_03126
Dates	13-AUG-2010 (Created) 1-JUN-2023 (Last updated, Version 10)

Name	Adenylosucc_synth_vert_basic

Scope

Eukaryota; Vertebrata

Templates

P28650 (PURA1_MOUSE); P0A7D4 (PURA_ECOLI): [Recover all]

case <OC:Eukaryota>

Triggered by

HAMAP; MF_03126 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PURA1

Protein name

RecName:		Full=Adenylosuccinate synthetase isozyme 1;
		Short=AMPSase 1;
		Short=AdSS 1;
		EC 6.3.4.4;
AltName:		Full=Adenylosuccinate synthetase, basic isozyme;
AltName:		Full=Adenylosuccinate synthetase, muscle isozyme;
		Short=M-type adenylosuccinate synthetase;
AltName:		Full=IMP--aspartate ligase 1;

Gene name

ADSSL1, ADSS1

Comments [?]

Function	Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
Catalytic activity	RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate EC 6.3.4.4
Cofactor	Mg(2+) Note: Binds 1 Mg(2+) ion per subunit.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit	Homodimer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the adenylosuccinate synthetase family.

Keywords [?]

Cytoplasm
GTP-binding
Ligase
Magnesium
Metal-binding
Nucleotide-binding
Purine biosynthesis

Gene Ontology [?]

GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS01266; ADENYLOSUCCIN_SYN_1; 1;
	PS00513; ADENYLOSUCCIN_SYN_2; 1;
Pfam	PF00709; Adenylsucc_synt; 1;
NCBIfam	TIGR00184; PurA; 1;

Features [?]

From: PURA1_MOUSE (P28650)

Key From To Description Tag Condition FTGroup

BINDING 42 48 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 G-D-E-G-K-G-K

BINDING 70 72 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 G-H-T

BINDING 363 365 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 K-L-D

BINDING 445 448 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 G-V-G-K

BINDING 43 46 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 D-E-G-K

BINDING 68 71 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 N-A-G-H

BINDING 331 337 /ligand="substrate x-x-T-x-[KR]-x-R

ACT_SITE 43 43 Proton acceptor D

ACT_SITE 71 71 Proton donor H

BINDING 43 43 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 70 70 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 G

BINDING 43 43 /ligand="substrate D

BINDING 163 163 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 T

BINDING 177 177 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" /ligand_note="ligand shared between dimeric partners R

BINDING 256 256 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 N

BINDING 271 271 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 T

BINDING 335 335 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 R

BINDING 337 337 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 R

Additional information [?]

Size range	449-459 amino acids
Related rules	MF_03125 (PURA); MF_03127 (PURA2)
Fusion	None

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