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Annotation rule MF_03126 |
Accession | MF_03126 |
Dates | 13-AUG-2010 (Created) 20-NOV-2019 (Last updated, Version 6) |
Name | Adenylosucc_synth_vert_basic |
Scope | Eukaryota; Vertebrata |
Templates | P28650 (PURA1_MOUSE); P0A7D4 (PURA_ECOLI): [Recover all] |
Triggered by |
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Protein name |
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Gene name |
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Function | Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP. |
Catalytic activity | RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate
EC 6.3.4.4 |
Cofactor | Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
Pathway | Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. |
Subunit | Homodimer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the adenylosuccinate synthetase family. |
PROSITE | PS01266; ADENYLOSUCCIN_SYN_1; 1; |
PS00513; ADENYLOSUCCIN_SYN_2; 1; | |
Pfam | PF00709; Adenylsucc_synt; 1; |
TIGRFAMs | TIGR00184; PurA; 1; |
From: PURA1_MOUSE (P28650) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND | 42 | 48 | GTP | G-D-E-G-K-G-K | ||||||||
NP_BIND | 70 | 72 | GTP | G-H-T | ||||||||
NP_BIND | 363 | 365 | GTP | K-L-D | ||||||||
NP_BIND | 445 | 448 | GTP | G-V-G-K | ||||||||
REGION | 43 | 46 | IMP binding | D-E-G-K | ||||||||
REGION | 68 | 71 | IMP binding | N-A-G-H | ||||||||
REGION | 331 | 337 | Substrate binding | x-x-T-x-[KR]-x-R | ||||||||
ACT_SITE | 43 | 43 | Proton acceptor | D | ||||||||
ACT_SITE | 71 | 71 | Proton donor | H | ||||||||
METAL | 43 | 43 | Magnesium | D | ||||||||
METAL | 70 | 70 | Magnesium; via carbonyl oxygen | G | ||||||||
BINDING | 43 | 43 | Substrate | D | ||||||||
BINDING | 163 | 163 | IMP | T | ||||||||
BINDING | 177 | 177 | IMP; shared with dimeric partner | R | ||||||||
BINDING | 256 | 256 | IMP | N | ||||||||
BINDING | 271 | 271 | IMP | T | ||||||||
BINDING | 335 | 335 | IMP | R | ||||||||
BINDING | 337 | 337 | GTP | R |