HAMAP rule MF_03126
General rule information
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Accession | MF_03126 |
Dates | 13-AUG-2010 (Created) 1-JUN-2023 (Last updated, Version 10) |
Name | Adenylosucc_synth_vert_basic |
Scope | Eukaryota; Vertebrata |
Templates | P28650 (PURA1_MOUSE); P0A7D4 (PURA_ECOLI): [Recover all] |
case <OC:Eukaryota>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP. |
Catalytic activity | RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate
EC 6.3.4.4 |
Cofactor | Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
Pathway | Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. |
Subunit | Homodimer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the adenylosuccinate synthetase family. |
Keywords
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Gene Ontology
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GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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PROSITE | PS01266; ADENYLOSUCCIN_SYN_1; 1; |
PS00513; ADENYLOSUCCIN_SYN_2; 1; | |
Pfam | PF00709; Adenylsucc_synt; 1; |
NCBIfam | TIGR00184; PurA; 1; |
Features
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From: PURA1_MOUSE (P28650) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 42 | 48 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | G-D-E-G-K-G-K | ||||||||
BINDING | 70 | 72 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | G-H-T | ||||||||
BINDING | 363 | 365 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | K-L-D | ||||||||
BINDING | 445 | 448 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | G-V-G-K | ||||||||
BINDING | 43 | 46 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | D-E-G-K | ||||||||
BINDING | 68 | 71 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | N-A-G-H | ||||||||
BINDING | 331 | 337 | /ligand="substrate | x-x-T-x-[KR]-x-R | ||||||||
ACT_SITE | 43 | 43 | Proton acceptor | D | ||||||||
ACT_SITE | 71 | 71 | Proton donor | H | ||||||||
BINDING | 43 | 43 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | ||||||||
BINDING | 70 | 70 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | G | ||||||||
BINDING | 43 | 43 | /ligand="substrate | D | ||||||||
BINDING | 163 | 163 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | T | ||||||||
BINDING | 177 | 177 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" /ligand_note="ligand shared between dimeric partners | R | ||||||||
BINDING | 256 | 256 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | N | ||||||||
BINDING | 271 | 271 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | T | ||||||||
BINDING | 335 | 335 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 | R | ||||||||
BINDING | 337 | 337 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | R |
Additional information
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