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HAMAP rule MF_03127

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General rule information [?]

Accession MF_03127
Dates 13-AUG-2010 (Created)
19-NOV-2022 (Last updated, Version 10)
Name Adenylosucc_synth_vert_acid
Eukaryota; Vertebrata
Templates P30520 (PURA2_HUMAN); P0A7D4 (PURA_ECOLI): [Recover all]
case <OC:Eukaryota>
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Adenylosuccinate synthetase isozyme 2;
Short=AMPSase 2;
Short=AdSS 2;
AltName: Full=Adenylosuccinate synthetase, acidic isozyme;
AltName: Full=Adenylosuccinate synthetase, liver isozyme;
Short=L-type adenylosuccinate synthetase;
AltName: Full=IMP--aspartate ligase 2;
Gene name

Comments [?]

Function Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
Catalytic activity RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
Pathway Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the adenylosuccinate synthetase family.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00709; Adenylsucc_synt; 1;
TIGRFAMs TIGR00184; PurA; 1;

Features [?]

case <OC:Mammalia>
From: PURA2_HUMAN (P30520)
Key     From     To       Description   Tag   Condition   FTGroup
INIT_MET (Optional)     1     1       Removed     M   1
CHAIN     2     Cter       Adenylosuccinate synthetase isozyme 2         1
MOD_RES (Optional)     2     2       N-acetylalanine     A   1
end case
BINDING     39     45       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     G-D-E-G-K-G-K  
BINDING     67     69       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     G-H-T  
BINDING     362     364       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     K-L-D  
BINDING     444     447       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     G-V-G-K  
BINDING     40     43       /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053     D-E-G-K  
BINDING     65     68       /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053     N-A-G-H  
BINDING     330     336       /ligand="substrate     x-x-T-x-[KR]-x-R  
ACT_SITE     40     40       Proton acceptor     D  
ACT_SITE     68     68       Proton donor     H  
BINDING     40     40       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D  
BINDING     67     67       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     G  
BINDING     40     40       /ligand="substrate     D  
BINDING     162     162       /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053     T  
BINDING     176     176       /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" /ligand_note="ligand shared between dimeric partners     R  
BINDING     255     255       /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053     N  
BINDING     270     270       /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053     T  
BINDING     334     334       /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053     R  
BINDING     336     336       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     R  

Additional information [?]

Size range 451-457 amino acids
Related rules MF_03125 (PURA); MF_03126 (PURA1)
Fusion None