HAMAP rule MF_03127
General rule information
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Accession | MF_03127 |
Dates | 13-AUG-2010 (Created)
1-JUN-2023 (Last updated, Version 11) |
Name | Adenylosucc_synth_vert_acid |
Scope(s) |
Eukaryota Vertebrata |
Template(s) | P30520 (PURA2_HUMAN); P0A7D4 (PURA_ECOLI); [ Recover all ] |
Triggered by |
case c? <OC:Eukaryota>
HAMAP; MF_03127 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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Identifier | PURA2 |
Protein name | RecName: Full=Adenylosuccinate synthetase isozyme 2; Short=AMPSase 2; Short=AdSS 2; EC=6.3.4.4; AltName: Full=Adenylosuccinate synthetase, acidic isozyme; AltName: Full=Adenylosuccinate synthetase, liver isozyme; Short=L-type adenylosuccinate synthetase; AltName: Full=IMP--aspartate ligase 2; |
Gene name | Name=ADSS; Synonyms=ADSS2; |
Comments
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FUNCTION | Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP. |
CATALYTIC ACTIVITY | Reaction=GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2- dicarboxyethyl)-AMP + phosphate; Xref=Rhea:RHEA:15753, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57567, ChEBI:CHEBI:58053, ChEBI:CHEBI:58189; EC=6.3.4.4; |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
PATHWAY | Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. |
SUBUNIT | Homodimer. |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the adenylosuccinate synthetase family. |
Keywords
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Cytoplasm | |
GTP-binding | |
Ligase | |
Magnesium | |
Metal-binding | |
Nucleotide-binding | |
Purine biosynthesis | |
case <FTGroup:1> | |
Acetylation | |
end case |
Gene Ontology
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GO:0004019; Molecular function:adenylosuccinate synthase activity |
GO:0005525; Molecular function:GTP binding |
GO:0000287; Molecular function:magnesium ion binding |
GO:0006164; Biological process:purine nucleotide biosynthetic process |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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PROSITE | PS01266; ADENYLOSUCCIN_SYN_1; 1; |
PROSITE | PS00513; ADENYLOSUCCIN_SYN_2; 1; |
Pfam | PF00709; Adenylsucc_synt; 1; |
NCBIfam | TIGR00184; PurA; 1; |
Features
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From: PURA2_HUMAN (P30520) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
case <OC:Mammalia> | ||||||||||||
INIT_MET | 1 | 1 | /note="Removed" | M | 1 | |||||||
CHAIN | 2 | Cter | /note="Adenylosuccinate synthetase isozyme 2" | 1 | ||||||||
MOD_RES | 2 | 2 | /note="N-acetylalanine" | A | 1 | |||||||
end case | ||||||||||||
BINDING | 39 | 45 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-D-E-G-K-G-K | ||||||||
BINDING | 67 | 69 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-H-T | ||||||||
BINDING | 362 | 364 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
K-L-D | ||||||||
BINDING | 444 | 447 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-V-G-K | ||||||||
BINDING | 40 | 43 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
D-E-G-K | ||||||||
BINDING | 65 | 68 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
N-A-G-H | ||||||||
BINDING | 330 | 336 | /ligand="substrate" | x-x-T-x-[KR]-x-R | ||||||||
ACT_SITE | 40 | 40 | /note="Proton acceptor" | D | ||||||||
ACT_SITE | 68 | 68 | /note="Proton donor" | H | ||||||||
BINDING | 40 | 40 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
BINDING | 67 | 67 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
G | ||||||||
BINDING | 40 | 40 | /ligand="substrate" | D | ||||||||
BINDING | 162 | 162 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
T | ||||||||
BINDING | 176 | 176 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" /ligand_note="ligand shared between dimeric partners" |
R | ||||||||
BINDING | 255 | 255 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
N | ||||||||
BINDING | 270 | 270 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
T | ||||||||
BINDING | 334 | 334 | /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" |
R | ||||||||
BINDING | 336 | 336 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
R |
Additional information
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Size range | 451-457 amino acids |
Related rules |
MF_03125 MF_03126 |
Fusion | Nter: None Cter: None |