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Annotation rule MF_03127
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General rule information [?]

Accession MF_03127
Dates 13-AUG-2010 (Created)
20-NOV-2019 (Last updated, Version 7)
Name Adenylosucc_synth_vert_acid
Scope
Eukaryota; Vertebrata
Templates P30520 (PURA2_HUMAN); P0A7D4 (PURA_ECOLI): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PURA2
Protein name
RecName: Full=Adenylosuccinate synthetase isozyme 2;
Short=AMPSase 2;
Short=AdSS 2;
EC 6.3.4.4;
AltName: Full=Adenylosuccinate synthetase, acidic isozyme;
AltName: Full=Adenylosuccinate synthetase, liver isozyme;
Short=L-type adenylosuccinate synthetase;
AltName: Full=IMP--aspartate ligase 2;
Gene name
ADSS, ADSS2

Comments [?]

Function Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
Catalytic activity RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate
EC 6.3.4.4
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
Pathway Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the adenylosuccinate synthetase family.

Keywords [?]

Cytoplasm
GTP-binding
Ligase
Magnesium
Metal-binding
Nucleotide-binding
Purine biosynthesis
case <FTGroup:1>
Acetylation
end case

Gene Ontology [?]

GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS01266; ADENYLOSUCCIN_SYN_1; 1;
PS00513; ADENYLOSUCCIN_SYN_2; 1;
Pfam PF00709; Adenylsucc_synt; 1;
TIGRFAMs TIGR00184; PurA; 1;

Features [?]

case <OC:Mammalia>
From: PURA2_HUMAN (P30520)
Key     From     To       Description   Tag   Condition   FTGroup
INIT_MET (Optional)     1     1       Removed     M   1
CHAIN     2     Cter       Adenylosuccinate synthetase isozyme 2         1
MOD_RES (Optional)     2     2       N-acetylalanine     A   1
end case
NP_BIND     39     45       GTP     G-D-E-G-K-G-K  
NP_BIND     67     69       GTP     G-H-T  
NP_BIND     362     364       GTP     K-L-D  
NP_BIND     444     447       GTP     G-V-G-K  
REGION     40     43       IMP binding     D-E-G-K  
REGION     65     68       IMP binding     N-A-G-H  
REGION     330     336       Substrate binding     x-x-T-x-[KR]-x-R  
ACT_SITE     40     40       Proton acceptor     D  
ACT_SITE     68     68       Proton donor     H  
METAL     40     40       Magnesium     D  
METAL     67     67       Magnesium; via carbonyl oxygen     G  
BINDING     40     40       Substrate     D  
BINDING     162     162       IMP     T  
BINDING     176     176       IMP; shared with dimeric partner     R  
BINDING     255     255       IMP     N  
BINDING     270     270       IMP     T  
BINDING     334     334       IMP     R  
BINDING     336     336       GTP     R  

Additional information [?]

Size range 451-457 amino acids
Related rules MF_03125 (PURA); MF_03126 (PURA1)
Fusion None


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