HAMAP rule MF_03127

General rule information [?]

Accession	MF_03127
Dates	13-AUG-2010 (Created) 19-NOV-2022 (Last updated, Version 10)

Name	Adenylosucc_synth_vert_acid

Scope

Eukaryota; Vertebrata

Templates

P30520 (PURA2_HUMAN); P0A7D4 (PURA_ECOLI): [Recover all]

case <OC:Eukaryota>

Triggered by

HAMAP; MF_03127 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PURA2

Protein name

RecName:		Full=Adenylosuccinate synthetase isozyme 2;
		Short=AMPSase 2;
		Short=AdSS 2;
		EC 6.3.4.4;
AltName:		Full=Adenylosuccinate synthetase, acidic isozyme;
AltName:		Full=Adenylosuccinate synthetase, liver isozyme;
		Short=L-type adenylosuccinate synthetase;
AltName:		Full=IMP--aspartate ligase 2;

Gene name

ADSS, ADSS2

Comments [?]

Function	Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
Catalytic activity	RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate EC 6.3.4.4
Cofactor	Mg(2+) Note: Binds 1 Mg(2+) ion per subunit.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit	Homodimer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the adenylosuccinate synthetase family.

Keywords [?]

Cytoplasm
GTP-binding
Ligase
Magnesium
Metal-binding
Nucleotide-binding
Purine biosynthesis

case <FTGroup:1>

Acetylation

end case

Gene Ontology [?]

GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS01266; ADENYLOSUCCIN_SYN_1; 1;
	PS00513; ADENYLOSUCCIN_SYN_2; 1;
Pfam	PF00709; Adenylsucc_synt; 1;
TIGRFAMs	TIGR00184; PurA; 1;

Features [?]

case <OC:Mammalia>

From: PURA2_HUMAN (P30520)

Key From To Description Tag Condition FTGroup

INIT_MET (Optional) 1 1 Removed M 1

CHAIN 2 Cter Adenylosuccinate synthetase isozyme 2 1

MOD_RES (Optional) 2 2 N-acetylalanine A 1

end case

BINDING 39 45 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 G-D-E-G-K-G-K

BINDING 67 69 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 G-H-T

BINDING 362 364 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 K-L-D

BINDING 444 447 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 G-V-G-K

BINDING 40 43 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 D-E-G-K

BINDING 65 68 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 N-A-G-H

BINDING 330 336 /ligand="substrate x-x-T-x-[KR]-x-R

ACT_SITE 40 40 Proton acceptor D

ACT_SITE 68 68 Proton donor H

BINDING 40 40 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 67 67 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 G

BINDING 40 40 /ligand="substrate D

BINDING 162 162 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 T

BINDING 176 176 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053" /ligand_note="ligand shared between dimeric partners R

BINDING 255 255 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 N

BINDING 270 270 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 T

BINDING 334 334 /ligand="IMP" /ligand_id="ChEBI:CHEBI:58053 R

BINDING 336 336 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 R

Additional information [?]

Size range	451-457 amino acids
Related rules	MF_03125 (PURA); MF_03126 (PURA1)
Fusion	None

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