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HAMAP rule MF_03128

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General rule information [?]

Accession MF_03128
Dates 13-AUG-2010 (Created)
1-JUN-2023 (Last updated, Version 16)
Name Lipoyl_synth_plantM
Eukaryota; Viridiplantae
Templates Q9ZWT1 (LIAS_ARATH); P32875 (LIPA_YEAST): [Recover all]
case <OC:Eukaryota>
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Lipoyl synthase, mitochondrial;
AltName: Full=Lipoate synthase;
AltName: Full=Lipoic acid synthase;
Gene name

Comments [?]

Function Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.
Catalytic activity RHEA:16585: [[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]
Cofactor [4Fe-4S] cluster
Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.
Subcellular location Mitochondrion.
case not <AnyFeature:TransitM>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
Similarity Belongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords [?]

Gene Ontology [?]

GO:0016783; Molecular function: sulfurtransferase activity.
GO:0016992; Molecular function: lipoate synthase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0009107; Biological process: lipoate biosynthetic process.
GO:0009249; Biological process: protein lipoylation.
GO:0005739; Cellular component: mitochondrion.

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
NCBIfam TIGR00510; LipA; 1;
PIRSF PIRSF005963; Lipoyl_synth; 1;

Computed features [?]

General TransitM; -; 0-1; trigger=yes;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
BINDING     103     103       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1     C  
BINDING     108     108       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1     C  
BINDING     114     114       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1     C  
BINDING     134     134       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet     C  
BINDING     138     138       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet     C  
BINDING     141     141       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet     C  
BINDING     350     350       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1     S  

Additional information [?]

Size range 374-399 amino acids
Related rules MF_03129 (LISC); MF_03123 (LIAS)
Fusion None