Annotation rule MF_03129
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General rule information [?]

Accession MF_03129
Dates 13-AUG-2010 (Created)
25-JAN-2017 (Last updated, Version 7)
Name Lipoyl_synth_plantC
Scope Eukaryota; Viridiplantae
Templates Q8LEE8 (LISC_ARATH); P32875 (LIPA_YEAST): [Recover all]
case <OC:Eukaryota>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
LISC
Protein name
RecName: Full=Lipoyl synthase, chloroplastic;
EC=2.8.1.8;
AltName: Full=Lipoate synthase;
Short=LS;
Short=Lip-syn;
AltName: Full=Lipoate synthase, plastidial;
Short=LIP1p;
AltName: Full=Lipoic acid synthase;
Gene name
LIP1P

Comments [?]

Function Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.
Catalytic activity Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.
Cofactor [4Fe-4S] cluster
Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.
Subcellular location Plastid, chloroplast.
case not <AnyFeature:TransitC>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
Similarity Belongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords [?]


Gene Ontology [?]

GO:0016783; Molecular function: sulfurtransferase activity.
GO:0016992; Molecular function: lipoate synthase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0009107; Biological process: lipoate biosynthetic process.
GO:0009249; Biological process: protein lipoylation.
GO:0009507; Cellular component: chloroplast.

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
TIGRFAMs TIGR00510; LipA; 1;
PIRSF PIRSF005963; Lipoyl_synth; 1;

Computed features [?]

General TransitC; -; 0-1; trigger=yes;

Features [?]

From: LISC_ARATH (Q8LEE8)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     127     127       Iron-sulfur 1 (4Fe-4S)     C  
METAL     132     132       Iron-sulfur 1 (4Fe-4S)     C  
METAL     138     138       Iron-sulfur 1 (4Fe-4S)     C  
METAL     158     158       Iron-sulfur 2 (4Fe-4S-S-AdoMet)     C  
METAL     162     162       Iron-sulfur 2 (4Fe-4S-S-AdoMet)     C  
METAL     165     165       Iron-sulfur 2 (4Fe-4S-S-AdoMet)     C  

Additional information [?]

Size range 324-430 amino acids
Related rules MF_03128 (LIAS); MF_03123 (LIAS)
Fusion None