HAMAP rule MF_03129

General rule information [?]

Accession	MF_03129
Dates	13-AUG-2010 (Created) 1-JUN-2023 (Last updated, Version 16)

Name	Lipoyl_synth_plantC

Scope

Eukaryota; Viridiplantae

Templates

Q8LEE8 (LISC_ARATH); P32875 (LIPA_YEAST): [Recover all]

case <OC:Eukaryota>

Triggered by

HAMAP; MF_03129 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

LISC

Protein name

RecName:		Full=Lipoyl synthase, chloroplastic;
		EC 2.8.1.8;
AltName:		Full=Lipoate synthase;
		Short=LS;
		Short=Lip-syn;
AltName:		Full=Lipoate synthase, plastidial;
		Short=LIP1p;
AltName:		Full=Lipoic acid synthase;

Gene name

LIP1P

Comments [?]

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.
Catalytic activity	RHEA:16585: [[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin] EC 2.8.1.8
Cofactor	[4Fe-4S] cluster Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.
Subcellular location	Plastid, chloroplast.

case not <AnyFeature:TransitC>

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.

end case

Similarity

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords [?]

4Fe-4S
Iron
Iron-sulfur
Metal-binding
Chloroplast
Plastid
S-adenosyl-L-methionine
Transferase

Gene Ontology [?]

GO:0016783; Molecular function: sulfurtransferase activity.
GO:0016992; Molecular function: lipoate synthase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0009107; Biological process: lipoate biosynthetic process.
GO:0009249; Biological process: protein lipoylation.
GO:0009507; Cellular component: chloroplast.

Cross-references [?]

Pfam	PF04055; Radical_SAM; 1;
NCBIfam	TIGR00510; LipA; 1;
PIRSF	PIRSF005963; Lipoyl_synth; 1;

Computed features [?]

General

TransitC; -; 0-1; trigger=yes;

Features [?]

From: LISC_ARATH (Q8LEE8)

Key From To Description Tag Condition FTGroup

BINDING 127 127 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C

BINDING 132 132 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C

BINDING 138 138 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C

BINDING 158 158 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet C

BINDING 162 162 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet C

BINDING 165 165 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet C

BINDING 373 373 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 S

Additional information [?]

Size range	324-430 amino acids
Related rules	MF_03128 (LIAS); MF_03123 (LIAS)
Fusion	None

View rule in raw text format (no links)