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HAMAP rule MF_03129

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General rule information [?]

Accession MF_03129
Dates 13-AUG-2010 (Created)
14-SEP-2023 (Last updated, Version 17)
Name Lipoyl_synth_plantC
Scope(s) Eukaryota
Viridiplantae
Template(s) Q8LEE8 (LISC_ARATH); P32875 (LIPA_YEAST); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_03129 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier LISC
Protein name RecName: Full=Lipoyl synthase, chloroplastic;
                 EC=2.8.1.8;
AltName: Full=Lipoate synthase;
                 Short=LS;
                 Short=Lip-syn;
AltName: Full=Lipoate synthase, plastidial;
                 Short=LIP1p;
AltName: Full=Lipoic acid synthase;
Gene name Name=LIP1P;

Comments [?]

FUNCTIONCatalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.
CATALYTIC ACTIVITY Reaction=[[Fe-S] cluster scaffold protein carrying a second [4Fe- 4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'- deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + N(6)-[(R)-dihydrolipoyl]-L-lysyl- [protein] + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:16585, Rhea:RHEA-COMP:9928, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:10475, Rhea:RHEA-COMP:14568, Rhea:RHEA-COMP:14569, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:29034, ChEBI:CHEBI:29919, ChEBI:CHEBI:33722, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:78809, ChEBI:CHEBI:83100; EC=2.8.1.8;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
PATHWAYProtein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2.
SUBCELLULAR LOCATIONPlastid, chloroplast.
case not <AnyFeature:TransitC>
MISCELLANEOUSThis protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITYBelongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords [?]

4Fe-4S
Iron
Iron-sulfur
Metal-binding
Chloroplast
Plastid
S-adenosyl-L-methionine
Transferase

Gene Ontology [?]

GO:0016783; Molecular function:sulfurtransferase activity
GO:0016992; Molecular function:lipoate synthase activity
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
GO:0009107; Biological process:lipoate biosynthetic process
GO:0009249; Biological process:protein lipoylation
GO:0009507; Cellular component:chloroplast

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
NCBIfam TIGR00510; LipA; 1;
PIRSF PIRSF005963; Lipoyl_synth; 1;
General TransitC; -; 0-1;

Features [?]

From: LISC_ARATH (Q8LEE8)
Key From To Description Tag Condition FTGroup
BINDING 127 127 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"		 C
BINDING 132 132 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"		 C
BINDING 138 138 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"		 C
BINDING 158 158 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"		 C
BINDING 162 162 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"		 C
BINDING 165 165 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"		 C
BINDING 373 373 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"		 S

Additional information [?]

Size range 324-430 amino acids
Related rules MF_03128
MF_03123
Fusion Nter: None Cter: None



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