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Annotation rule MF_03134
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General rule information [?]

Accession MF_03134
Dates 22-OCT-2010 (Created)
20-NOV-2019 (Last updated, Version 6)
Name Ala_tRNA_synth_plantC
Scope
Eukaryota; Viridiplantae
Template Q9FFC7 (SYAP_ARATH)
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYAP
Protein name
RecName: Full=Probable alanine--tRNA ligase, chloroplastic;
EC 6.1.1.7;
AltName: Full=Alanyl-tRNA synthetase;
Short=AlaRS;

Comments [?]

Function Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Catalytic activity RHEA:12540: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
EC 6.1.1.7
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
Subunit Monomer.
Subcellular location Plastid, chloroplast. Mitochondrion.
Domain Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
Similarity Belongs to the class-II aminoacyl-tRNA synthetase family.

Keywords [?]

Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis
RNA-binding
tRNA-binding
Plastid
Chloroplast
Mitochondrion
case <FTGroup:1>
Metal-binding
Zinc
end case

Gene Ontology [?]

GO:0004813; Molecular function: alanine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0006419; Biological process: alanyl-tRNA aminoacylation.
GO:0009507; Cellular component: chloroplast.
GO:0005739; Cellular component: mitochondrion.

Cross-references [?]

Pfam PF02272; DHHA1; 1;
PF01411; tRNA-synt_2c; 1;
PF07973; tRNA_SAD; 1;
PRINTS PR00980; TRNASYNTHALA; 1;
TIGRFAMs TIGR00344; AlaS; 1;
PROSITE PS50860; AA_TRNA_LIGASE_II_ALA; 1;

Computed features [?]

General TransitC; -; 0-1; trigger=yes;

Features [?]

From: SYAP_ARATH (Q9FFC7)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     655     655       Zinc     H   1
METAL     659     659       Zinc     H   1
METAL     758     758       Zinc     C   1
METAL     762     762       Zinc     H   1

Additional information [?]

Size range 815-996 amino acids
Related rules MF_03133 (SYAC)
Fusion None


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