HAMAP rule MF_03134
General rule information
[?]
Accession | MF_03134 |
Dates | 22-OCT-2010 (Created) 19-NOV-2022 (Last updated, Version 8) |
Name | Ala_tRNA_synth_plantC |
Scope | Eukaryota; Viridiplantae |
Template | Q9FFC7 (SYAP_ARATH) |
case <OC:Eukaryota>
Triggered by |
end case
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier |
|
Protein name |
|
Comments
[?]
Function | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. |
Catalytic activity | RHEA:12540: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
EC 6.1.1.7 |
Cofactor | Zn(2+) Note: Binds 1 zinc ion per subunit. |
Subunit | Monomer. |
Subcellular location | Plastid, chloroplast. Mitochondrion. |
Domain | Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. |
Similarity | Belongs to the class-II aminoacyl-tRNA synthetase family. |
Keywords
[?]
Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis
RNA-binding
tRNA-binding
Plastid
Chloroplast
Mitochondrion
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis
RNA-binding
tRNA-binding
Plastid
Chloroplast
Mitochondrion
case <FTGroup:1>
end case
Gene Ontology
[?]
GO:0004813; Molecular function: alanine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0006419; Biological process: alanyl-tRNA aminoacylation.
GO:0009507; Cellular component: chloroplast.
GO:0005739; Cellular component: mitochondrion.
GO:0005524; Molecular function: ATP binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0006419; Biological process: alanyl-tRNA aminoacylation.
GO:0009507; Cellular component: chloroplast.
GO:0005739; Cellular component: mitochondrion.
Cross-references
[?]
Pfam | PF02272; DHHA1; 1; |
PF01411; tRNA-synt_2c; 1; | |
PF07973; tRNA_SAD; 1; | |
PRINTS | PR00980; TRNASYNTHALA; 1; |
TIGRFAMs | TIGR00344; AlaS; 1; |
PROSITE | PS50860; AA_TRNA_LIGASE_II_ALA; 1; |
Computed features
[?]
General | TransitC; -; 0-1; trigger=yes; |
Features
[?]
From: SYAP_ARATH (Q9FFC7) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 655 | 655 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | H | 1 | |||||||
BINDING | 659 | 659 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | H | 1 | |||||||
BINDING | 758 | 758 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | 1 | |||||||
BINDING | 762 | 762 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | H | 1 |
Additional information
[?]
Size range | 815-996 amino acids |
Related rules | MF_03133 (SYAC) |
Fusion | None |