HAMAP rule MF_03134
General rule information
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| Accession | MF_03134 |
| Dates | 22-OCT-2010 (Created)
3-SEP-2024 (Last updated, Version 10) |
| Name | Ala_tRNA_synth_plantC |
| Scope(s) |
Eukaryota Viridiplantae |
| Template(s) | Q9FFC7 (SYAP_ARATH); [ Recover all ] |
| Triggered by |
case c? <OC:Eukaryota>
HAMAP; MF_03134 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYAP |
| Protein name | RecName: Full=Probable alanine--tRNA ligase, chloroplastic; EC=6.1.1.7; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS; |
Comments
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| FUNCTION | Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. |
| CATALYTIC ACTIVITY | Reaction=tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate; Xref=Rhea:RHEA:12540, Rhea:RHEA-COMP:9657, Rhea:RHEA- COMP:9923, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57972, ChEBI:CHEBI:78442, ChEBI:CHEBI:78497, ChEBI:CHEBI:456215; EC=6.1.1.7; |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Plastid, chloroplast. Mitochondrion. |
| DOMAIN | Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. |
| SIMILARITY | Belongs to the class-II aminoacyl-tRNA synthetase family. |
Keywords
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| Aminoacyl-tRNA synthetase | |
| ATP-binding | |
| Ligase | |
| Nucleotide-binding | |
| Protein biosynthesis | |
| RNA-binding | |
| tRNA-binding | |
| Plastid | |
| Chloroplast | |
| Mitochondrion | |
| case <FTGroup:1> | |
| Metal-binding | |
| Zinc | |
| end case | |
Gene Ontology
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| GO:0004813; Molecular function:alanine-tRNA ligase activity |
| GO:0005524; Molecular function:ATP binding |
| GO:0008270; Molecular function:zinc ion binding |
| GO:0006419; Biological process:alanyl-tRNA aminoacylation |
| GO:0009507; Cellular component:chloroplast |
| GO:0005739; Cellular component:mitochondrion |
Cross-references
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| Pfam | PF02272; DHHA1; 1; |
| Pfam | PF01411; tRNA-synt_2c; 1; |
| Pfam | PF07973; tRNA_SAD; 1; |
| PRINTS | PR00980; TRNASYNTHALA; 1; |
| NCBIfam | TIGR00344; AlaS; 1; |
| PROSITE | PS50860; AA_TRNA_LIGASE_II_ALA; 1; |
| General | TransitC; -; 0-1; |
Features
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| From: SYAP_ARATH (Q9FFC7) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 655 | 655 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | 1 | |||||||
| BINDING | 659 | 659 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | 1 | |||||||
| BINDING | 758 | 758 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 762 | 762 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | 1 | |||||||
Additional information
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| Size range | 815-996 amino acids |
| Related rules |
MF_03133 |
| Fusion | Nter: None Cter: None |