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Annotation rule MF_03134 |
Accession | MF_03134 |
Dates | 22-OCT-2010 (Created) 20-NOV-2019 (Last updated, Version 6) |
Name | Ala_tRNA_synth_plantC |
Scope | Eukaryota; Viridiplantae |
Template | Q9FFC7 (SYAP_ARATH) |
Triggered by |
Identifier |
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Protein name |
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Function | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. |
Catalytic activity | RHEA:12540: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
EC 6.1.1.7 |
Cofactor | Zn(2+) Note: Binds 1 zinc ion per subunit. |
Subunit | Monomer. |
Subcellular location | Plastid, chloroplast. Mitochondrion. |
Domain | Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. |
Similarity | Belongs to the class-II aminoacyl-tRNA synthetase family. |
Pfam | PF02272; DHHA1; 1; |
PF01411; tRNA-synt_2c; 1; | |
PF07973; tRNA_SAD; 1; | |
PRINTS | PR00980; TRNASYNTHALA; 1; |
TIGRFAMs | TIGR00344; AlaS; 1; |
PROSITE | PS50860; AA_TRNA_LIGASE_II_ALA; 1; |
General | TransitC; -; 0-1; trigger=yes; |
From: SYAP_ARATH (Q9FFC7) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
METAL | 655 | 655 | Zinc | H | 1 | |||||||
METAL | 659 | 659 | Zinc | H | 1 | |||||||
METAL | 758 | 758 | Zinc | C | 1 | |||||||
METAL | 762 | 762 | Zinc | H | 1 |
Size range | 815-996 amino acids |
Related rules | MF_03133 (SYAC) |
Fusion | None |