HAMAP rule MF_03134

General rule information [?]

Accession	MF_03134
Dates	22-OCT-2010 (Created) 1-JUN-2023 (Last updated, Version 9)

Name	Ala_tRNA_synth_plantC

Scope

Eukaryota; Viridiplantae

Template

Q9FFC7 (SYAP_ARATH)

case <OC:Eukaryota>

Triggered by

HAMAP; MF_03134 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

SYAP

Protein name

RecName:		Full=Probable alanine--tRNA ligase, chloroplastic;
		EC 6.1.1.7;
AltName:		Full=Alanyl-tRNA synthetase;
		Short=AlaRS;

Comments [?]

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Catalytic activity	RHEA:12540: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala) EC 6.1.1.7
Cofactor	Zn(2+) Note: Binds 1 zinc ion per subunit.
Subunit	Monomer.
Subcellular location	Plastid, chloroplast. Mitochondrion.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
Similarity	Belongs to the class-II aminoacyl-tRNA synthetase family.

Keywords [?]

Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis
RNA-binding
tRNA-binding
Plastid
Chloroplast
Mitochondrion

case <FTGroup:1>

Metal-binding
Zinc

end case

Gene Ontology [?]

GO:0004813; Molecular function: alanine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0006419; Biological process: alanyl-tRNA aminoacylation.
GO:0009507; Cellular component: chloroplast.
GO:0005739; Cellular component: mitochondrion.

Cross-references [?]

Pfam	PF02272; DHHA1; 1;
	PF01411; tRNA-synt_2c; 1;
	PF07973; tRNA_SAD; 1;
PRINTS	PR00980; TRNASYNTHALA; 1;
NCBIfam	TIGR00344; AlaS; 1;
PROSITE	PS50860; AA_TRNA_LIGASE_II_ALA; 1;

Computed features [?]

General

TransitC; -; 0-1; trigger=yes;

Features [?]

From: SYAP_ARATH (Q9FFC7)

Key From To Description Tag Condition FTGroup

BINDING 655 655 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 H 1

BINDING 659 659 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 H 1

BINDING 758 758 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C 1

BINDING 762 762 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 H 1

Additional information [?]

Size range	815-996 amino acids
Related rules	MF_03133 (SYAC)
Fusion	None

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