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HAMAP rule MF_03134

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General rule information [?]

Accession MF_03134
Dates 22-OCT-2010 (Created)
1-JUN-2023 (Last updated, Version 9)
Name Ala_tRNA_synth_plantC
Scope(s) Eukaryota
Template(s) Q9FFC7 (SYAP_ARATH); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_03134 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYAP
Protein name RecName: Full=Probable alanine--tRNA ligase, chloroplastic;
AltName: Full=Alanyl-tRNA synthetase;

Comments [?]

FUNCTIONCatalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
CATALYTIC ACTIVITY Reaction=ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl- tRNA(Ala); Xref=Rhea:RHEA:12540, Rhea:RHEA-COMP:9657, Rhea:RHEA- COMP:9923, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57972, ChEBI:CHEBI:78442, ChEBI:CHEBI:78497, ChEBI:CHEBI:456215; EC=;
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
SUBCELLULAR LOCATIONPlastid, chloroplast. Mitochondrion.
DOMAINConsists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
SIMILARITYBelongs to the class-II aminoacyl-tRNA synthetase family.

Keywords [?]

Gene Ontology [?]

GO:0004813; Molecular function:alanine-tRNA ligase activity
GO:0005524; Molecular function:ATP binding
GO:0008270; Molecular function:zinc ion binding
GO:0006419; Biological process:alanyl-tRNA aminoacylation
GO:0009507; Cellular component:chloroplast
GO:0005739; Cellular component:mitochondrion

Cross-references [?]

Pfam PF02272; DHHA1; 1;
Pfam PF01411; tRNA-synt_2c; 1;
Pfam PF07973; tRNA_SAD; 1;
NCBIfam TIGR00344; AlaS; 1;
General TransitC; -; 0-1;

Features [?]

Key From To Description Tag Condition FTGroup
BINDING 655 655 /ligand="Zn(2+)"
H 1
BINDING 659 659 /ligand="Zn(2+)"
H 1
BINDING 758 758 /ligand="Zn(2+)"
C 1
BINDING 762 762 /ligand="Zn(2+)"
H 1

Additional information [?]

Size range 815-996 amino acids
Related rules MF_03133
Fusion Nter: None Cter: None

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