HAMAP rule MF_03134
General rule information
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Accession | MF_03134 |
Dates | 22-OCT-2010 (Created)
1-JUN-2023 (Last updated, Version 9) |
Name | Ala_tRNA_synth_plantC |
Scope(s) |
Eukaryota Viridiplantae |
Template(s) | Q9FFC7 (SYAP_ARATH); [ Recover all ] |
Triggered by |
case c? <OC:Eukaryota>
HAMAP; MF_03134 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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Identifier | SYAP |
Protein name | RecName: Full=Probable alanine--tRNA ligase, chloroplastic; EC=6.1.1.7; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS; |
Comments
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FUNCTION | Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. |
CATALYTIC ACTIVITY | Reaction=ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl- tRNA(Ala); Xref=Rhea:RHEA:12540, Rhea:RHEA-COMP:9657, Rhea:RHEA- COMP:9923, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57972, ChEBI:CHEBI:78442, ChEBI:CHEBI:78497, ChEBI:CHEBI:456215; EC=6.1.1.7; |
COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
SUBUNIT | Monomer. |
SUBCELLULAR LOCATION | Plastid, chloroplast. Mitochondrion. |
DOMAIN | Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs. |
SIMILARITY | Belongs to the class-II aminoacyl-tRNA synthetase family. |
Keywords
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Aminoacyl-tRNA synthetase | |
ATP-binding | |
Ligase | |
Nucleotide-binding | |
Protein biosynthesis | |
RNA-binding | |
tRNA-binding | |
Plastid | |
Chloroplast | |
Mitochondrion | |
case <FTGroup:1> | |
Metal-binding | |
Zinc | |
end case |
Gene Ontology
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GO:0004813; Molecular function:alanine-tRNA ligase activity |
GO:0005524; Molecular function:ATP binding |
GO:0008270; Molecular function:zinc ion binding |
GO:0006419; Biological process:alanyl-tRNA aminoacylation |
GO:0009507; Cellular component:chloroplast |
GO:0005739; Cellular component:mitochondrion |
Cross-references
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Pfam | PF02272; DHHA1; 1; |
Pfam | PF01411; tRNA-synt_2c; 1; |
Pfam | PF07973; tRNA_SAD; 1; |
PRINTS | PR00980; TRNASYNTHALA; 1; |
NCBIfam | TIGR00344; AlaS; 1; |
PROSITE | PS50860; AA_TRNA_LIGASE_II_ALA; 1; |
General | TransitC; -; 0-1; |
Features
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From: SYAP_ARATH (Q9FFC7) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 655 | 655 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | 1 | |||||||
BINDING | 659 | 659 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | 1 | |||||||
BINDING | 758 | 758 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
BINDING | 762 | 762 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | 1 |
Additional information
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Size range | 815-996 amino acids |
Related rules |
MF_03133 |
Fusion | Nter: None Cter: None |