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Annotation rule MF_03144 |
Accession | MF_03144 |
Dates | 9-MAR-2011 (Created) 20-NOV-2019 (Last updated, Version 8) |
Name | RtcB_euk |
Scope | Eukaryota |
Template | Q9Y3I0 (RTCB_HUMAN) |
Triggered by |
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Function | Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs. |
Catalytic activity | Reaction=(Ribonucleotide)(n)-3'-phosphate + 5'-hydroxy-(ribonucleotide)(m) + GTP = (ribonucleotide)(n+m) + GMP + diphosphate.; EC=6.5.1.8; |
Reaction=(Ribonucleotide)(n)-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)(m) + GTP + H(2)O = (ribonucleotide)(n+m) + GMP + diphosphate (overall reaction).; EC=6.5.1.8; | |
Cofactor | Mn(2+) Note: Binds 2 manganese ions per subunit |
Subunit | Catalytic component of the tRNA-splicing ligase complex. |
Subcellular location | Cytoplasm. |
Miscellaneous | Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP. |
Similarity | Belongs to the RtcB family. |
From: RTCB_HUMAN (Q9Y3I0) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND | 226 | 230 | GMP | N-H-Y-x-E | ||||||||
NP_BIND | 353 | 354 | GMP | H-N | ||||||||
NP_BIND | 402 | 405 | GMP | G-G-[TS]-M | ||||||||
NP_BIND | 428 | 431 | GMP | H-G-[AS]-G | ||||||||
ACT_SITE | 428 | 428 | GMP-histidine intermediate | H | ||||||||
METAL | 119 | 119 | Manganese 1 | D | ||||||||
METAL | 122 | 122 | Manganese 1 | C | ||||||||
METAL | 122 | 122 | Manganese 2 | C | ||||||||
METAL | 227 | 227 | Manganese 1; via tele nitrogen | H | ||||||||
METAL | 259 | 259 | Manganese 2; via tele nitrogen | H | ||||||||
METAL | 353 | 353 | Manganese 2; via tele nitrogen | H | ||||||||
BINDING | 409 | 409 | GMP | S | ||||||||
BINDING | 504 | 504 | GMP | K |
Size range | 457-535 amino acids |
Related rules | None |
Fusion | None |