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Annotation rule MF_03144
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General rule information [?]

Accession MF_03144
Dates 9-MAR-2011 (Created)
20-NOV-2019 (Last updated, Version 8)
Name RtcB_euk
Scope
Eukaryota
Template Q9Y3I0 (RTCB_HUMAN)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RTCB
Protein name
RecName: Full=RNA-splicing ligase RtcB homolog;
EC 6.5.1.8;
AltName: Full=3'-phosphate/5'-hydroxy nucleic acid ligase;
case <OC:Vertebrata>
Gene name
RTCB
end case

Comments [?]

Function Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Catalytic activity Reaction=(Ribonucleotide)(n)-3'-phosphate + 5'-hydroxy-(ribonucleotide)(m) + GTP = (ribonucleotide)(n+m) + GMP + diphosphate.; EC=6.5.1.8;
Reaction=(Ribonucleotide)(n)-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)(m) + GTP + H(2)O = (ribonucleotide)(n+m) + GMP + diphosphate (overall reaction).; EC=6.5.1.8;
Cofactor Mn(2+)
Note: Binds 2 manganese ions per subunit
Subunit Catalytic component of the tRNA-splicing ligase complex.
case <OC:Vertebrata>
Subcellular location Cytoplasm.
end case
Miscellaneous Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.
Similarity Belongs to the RtcB family.

Cross-references [?]

PROSITE PS01288; UPF0027; 1;
Pfam PF01139; RtcB; 1;

Keywords [?]

case <OC:Vertebrata>
Cytoplasm
end case
GTP-binding
Ligase
Manganese
Metal-binding
Nucleotide-binding
tRNA processing

Gene Ontology [?]

GO:0072669; Cellular component: tRNA-splicing ligase complex.
GO:0016886; Molecular function: ligase activity, forming phosphoric ester bonds.
GO:0006388; Biological process: tRNA splicing, via endonucleolytic cleavage and ligation.

Features [?]

From: RTCB_HUMAN (Q9Y3I0)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     226     230       GMP     N-H-Y-x-E  
NP_BIND     353     354       GMP     H-N  
NP_BIND     402     405       GMP     G-G-[TS]-M  
NP_BIND     428     431       GMP     H-G-[AS]-G  
ACT_SITE     428     428       GMP-histidine intermediate     H  
METAL     119     119       Manganese 1     D  
METAL     122     122       Manganese 1     C  
METAL     122     122       Manganese 2     C  
METAL     227     227       Manganese 1; via tele nitrogen     H  
METAL     259     259       Manganese 2; via tele nitrogen     H  
METAL     353     353       Manganese 2; via tele nitrogen     H  
BINDING     409     409       GMP     S  
BINDING     504     504       GMP     K  

Additional information [?]

Size range 457-535 amino acids
Related rules None
Fusion None


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