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HAMAP rule MF_03148

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_03148
Accession MF_03148
Dates 05-NOV-2012 (Created)
21-FEB-2025 (Last updated, Version 17)
Name HAM1_NTPase
Scope(s) Eukaryota
Template(s) Q9BY32; Q9D892; P47119; [ Recover all ]
Triggered by HAMAP; MF_03148 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ITPA
Protein name RecName: Full=Inosine triphosphate pyrophosphatase;
                 Short=ITPase;
                 Short=Inosine triphosphatase;
                 EC=3.6.1.66;
AltName: Full=Non-canonical purine NTP pyrophosphatase;
AltName: Full=Non-standard purine NTP pyrophosphatase;
AltName: Full=Nucleoside-triphosphate diphosphatase;
AltName: Full=Nucleoside-triphosphate pyrophosphatase;
                 Short=NTPase;
AltName: Full=XTP/dITP diphosphatase;
case <OC:Vertebrata>
Gene name Name=ITPA;
else case <OC:Caenorhabditis>
Gene name Name=hap-1;
else case <OC:Saccharomycotina>
Gene name Name=HAM1;
end case

Comments [?]

case <OC:Vertebrata>
FUNCTIONPyrophosphatase that hydrolyzes the non-canonical purine (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
else case <OC:Saccharomyces>
FUNCTIONPyrophosphatase that hydrolyzes the non-canonical purine (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and 5-bromodeoxyuridine 5'-triphosphate (BrdUTP) to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
else
FUNCTIONPyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
end case
CATALYTIC ACTIVITY Reaction=ITP + H2O = IMP + diphosphate + H(+); Xref=Rhea:RHEA:29399, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:58053, ChEBI:CHEBI:61402; EC=3.6.1.66; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29400;
CATALYTIC ACTIVITY Reaction=dITP + H2O = dIMP + diphosphate + H(+); Xref=Rhea:RHEA:28342, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:61194, ChEBI:CHEBI:61382; EC=3.6.1.66; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28343;
CATALYTIC ACTIVITY Reaction=XTP + H2O = XMP + diphosphate + H(+); Xref=Rhea:RHEA:28610, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:57464, ChEBI:CHEBI:61314; EC=3.6.1.66; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28611;
case <OC:Vertebrata> or <OC:Saccharomyces>
CATALYTIC ACTIVITY Reaction=N(6)-hydroxy-dATP + H2O = N(6)-hydroxy-dAMP + diphosphate + H(+); Xref=Rhea:RHEA:83971, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:233529, ChEBI:CHEBI:233530; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83972;
end case
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).;
SUBUNITHomodimer.
case <OC:Fungi>
SUBCELLULAR LOCATIONCytoplasm. Nucleus.
else
SUBCELLULAR LOCATIONCytoplasm.
end case
SIMILARITYBelongs to the HAM1 NTPase family.

Keywords [?]

case <FTGroup:1>
Acetylation
end case
case <OC:Fungi>
Nucleus
end case
Cytoplasm
Hydrolase
Magnesium
Manganese
Metal-binding
Nucleotide metabolism
Nucleotide-binding

Gene Ontology [?]

case <OCellular component:Fungi>
GO:0005634; Cellular component:nucleus
end case
GO:0005737; Cellular component:cytoplasm
GO:0047429; Molecular function:nucleoside triphosphate diphosphatase activity
GO:0036220; Molecular function:ITP diphosphatase activity
GO:0035870; Molecular function:dITP diphosphatase activity
GO:0036222; Molecular function:XTP diphosphatase activity
GO:0009204; Biological process:deoxyribonucleoside triphosphate catabolic process

Cross-references [?]

Pfam PF01725; Ham1p_like; 1;
NCBIfam TIGR00042; TIGR00042; 1;

Features [?]

From: ITPA_HUMAN (Q9BY32)
Key From To Description Tag Condition FTGroup
case <OC:Mammalia>
INIT_MET Nter Nter /note="Removed" M 1
MOD_RES Nter+1 Nter+1 /note="N-acetylalanine" A 1
end case
BINDING 14 19 /ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"		 T-x-N-x-x-K
BINDING 72 73 /ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"		 D-[TS]
BINDING 149 152 /ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"		 F-G-W-[DEN]
BINDING 177 178 /ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"		 H-R
BINDING 44 44 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 E
BINDING 72 72 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D
BINDING 56 56 /ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"		 K
BINDING 172 172 /ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"		 [KR]

Additional information [?]

Size range 183-234 amino acids
Related rules None
Fusion Nter: None Cter: None



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