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Annotation rule MF_03148
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General rule information [?]

Accession MF_03148
Dates 9-AUG-2011 (Created)
20-NOV-2019 (Last updated, Version 7)
Name HAM1_NTPase
Scope
Eukaryota
Templates Q9BY32 (ITPA_HUMAN); Q9D892 (ITPA_MOUSE); P47119 (ITPA_YEAST): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ITPA
Protein name
RecName: Full=Inosine triphosphate pyrophosphatase;
Short=ITPase;
Short=Inosine triphosphatase;
EC 3.6.1.9;
AltName: Full=Non-canonical purine NTP pyrophosphatase;
AltName: Full=Non-standard purine NTP pyrophosphatase;
AltName: Full=Nucleoside-triphosphate diphosphatase;
AltName: Full=Nucleoside-triphosphate pyrophosphatase;
Short=NTPase;
case <OC:Vertebrata>
Gene name
ITPA
else case <OC:Caenorhabditis>
Gene name
hap-1
else case <OC:Saccharomycotina>
Gene name
HAM1
end case

Comments [?]

case <OC:Vertebrata>
Function Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
else case <OC:Saccharomyces>
Function Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and 5-bromodeoxyuridine 5'-triphosphate (BrdUTP) to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
else
Function Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
end case
Catalytic activity RHEA:23996: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H(+)
EC 3.6.1.9
RHEA:44644: a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside 5'-phosphate + diphosphate + H(+)
EC 3.6.1.9
Cofactor Mg(2+)
Mn(2+)
Note: Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).
Subunit Homodimer.
case <OC:Fungi>
Subcellular location Cytoplasm. Nucleus.
else
Subcellular location Cytoplasm.
end case
Similarity Belongs to the HAM1 NTPase family.

Keywords [?]

case <OC:Mammalia>
end case
case <OC:Fungi>
end case

Gene Ontology [?]

case <OC:Fungi>
GO:0005634; Cellular component: nucleus.
end case
GO:0005737; Cellular component: cytoplasm.
GO:0047429; Molecular function: nucleoside-triphosphate diphosphatase activity.
GO:0009204; Biological process: deoxyribonucleoside triphosphate catabolic process.

Cross-references [?]

Pfam PF01725; Ham1p_like; 1;
TIGRFAMs TIGR00042; TIGR00042; 1;

Features [?]

case <OC:Mammalia>
From: ITPA_HUMAN (Q9BY32)
Key     From     To       Description   Tag   Condition   FTGroup
INIT_MET     Nter     Nter       Removed        
MOD_RES     2     2       N-acetylalanine     A  
end case
REGION     14     19       Substrate binding     T-x-N-x-x-K  
REGION     72     73       Substrate binding     D-[TS]  
REGION     149     152       Substrate binding     F-G-W-[DEN]  
REGION     177     178       Substrate binding     H-R  
METAL     44     44       Magnesium or manganese     E  
METAL     72     72       Magnesium or manganese     D  
BINDING     56     56       Substrate     K  
BINDING     172     172       Substrate     [KR]  

Additional information [?]

Size range 183-234 amino acids
Related rules None
Fusion None