 |
|
Annotation rule MF_03148 |
General rule information
[?]
Accession |
MF_03148 |
Dates |
9-AUG-2011 (Created) 20-NOV-2019 (Last updated, Version 7) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
|
Full=Inosine triphosphate pyrophosphatase;
|
|
Short=ITPase;
|
|
Short=Inosine triphosphatase;
|
|
EC 3.6.1.9;
|
AltName:
|
Full=Non-canonical purine NTP pyrophosphatase;
|
AltName:
|
Full=Non-standard purine NTP pyrophosphatase;
|
AltName:
|
Full=Nucleoside-triphosphate diphosphatase;
|
AltName:
|
Full=Nucleoside-triphosphate pyrophosphatase;
|
|
Short=NTPase;
|
|
case <OC:Vertebrata>
else case <OC:Caenorhabditis>
else case <OC:Saccharomycotina>
end case
case <OC:Vertebrata>
Function |
Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. |
else case <OC:Saccharomyces>
Function |
Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and 5-bromodeoxyuridine 5'-triphosphate (BrdUTP) to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. |
else
Function |
Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. |
end case
Catalytic activity |
RHEA:23996: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H(+)
EC 3.6.1.9
|
|
RHEA:44644: a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside 5'-phosphate + diphosphate + H(+)
EC 3.6.1.9
|
Cofactor |
Mg(2+) Mn(2+) Note: Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+). |
Subunit |
Homodimer. |
case <OC:Fungi>
Subcellular location |
Cytoplasm. Nucleus. |
else
Subcellular location |
Cytoplasm. |
end case
Similarity |
Belongs to the HAM1 NTPase family. |
case <OC:Mammalia>
end case
case <OC:Fungi>
end case
case <OC:Fungi>
end case
GO:0005737; Cellular component: cytoplasm.
GO:0047429; Molecular function: nucleoside-triphosphate diphosphatase activity.
GO:0009204; Biological process: deoxyribonucleoside triphosphate catabolic process.
case <OC:Mammalia>
From: ITPA_HUMAN (Q9BY32) |
Key
|
|
From
|
|
To
|
|
Description
|
|
Tag
|
|
Condition
|
|
FTGroup
|
INIT_MET
|
|
Nter
|
|
Nter
|
|
Removed
|
|
|
|
|
|
|
MOD_RES
|
|
2
|
|
2
|
|
N-acetylalanine
|
|
|
|
A
|
|
|
end case
REGION
|
|
14
|
|
19
|
|
Substrate binding
|
|
|
|
T-x-N-x-x-K
|
|
|
REGION
|
|
72
|
|
73
|
|
Substrate binding
|
|
|
|
D-[TS]
|
|
|
REGION
|
|
149
|
|
152
|
|
Substrate binding
|
|
|
|
F-G-W-[DEN]
|
|
|
REGION
|
|
177
|
|
178
|
|
Substrate binding
|
|
|
|
H-R
|
|
|
METAL
|
|
44
|
|
44
|
|
Magnesium or manganese
|
|
|
|
E
|
|
|
METAL
|
|
72
|
|
72
|
|
Magnesium or manganese
|
|
|
|
D
|
|
|
BINDING
|
|
56
|
|
56
|
|
Substrate
|
|
|
|
K
|
|
|
BINDING
|
|
172
|
|
172
|
|
Substrate
|
|
|
|
[KR]
|
|
|
Additional information
[?]
Size range |
183-234 amino acids |
Related rules |
None |
Fusion |
None |