HAMAP rule MF_03151

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_03151
Accession	MF_03151
Dates	06-NOV-2012 (Created) 03-SEP-2024 (Last updated, Version 8)
Name	GatF
Scope(s)	Eukaryota Saccharomycotina
Template(s)	P53260; [ Recover all ]
Triggered by	HAMAP; MF_03151 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	GATF
Protein name	RecName: Full=Glutamyl-tRNA(Gln) amidotransferase subunit F, mitochondrial; Short=Glu-AdT subunit F; EC=6.3.5.-;
Gene name	Name=GTF1;

Comments [?]

FUNCTION	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Required for proper protein synthesis within the mitochondrion.
CATALYTIC ACTIVITY	Reaction=L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl- tRNA(Gln) + L-glutamate + ADP + phosphate + H(+); Xref=Rhea:RHEA:17521, Rhea:RHEA-COMP:9681, Rhea:RHEA-COMP:9684, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:78520, ChEBI:CHEBI:78521, ChEBI:CHEBI:456216;
SUBUNIT	Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A, B and F subunits.
SUBCELLULAR LOCATION	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
case not <AnyFeature:TransitM>
MISCELLANEOUS	This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITY	Belongs to the GatF family.

Keywords [?]

Gene Ontology [?]

GO:0005739; Cellular component:mitochondrion

GO:0030956; Cellular component:glutamyl-tRNA(Gln) amidotransferase complex

GO:0050567; Molecular function:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity

GO:0016884; Molecular function:carbon-nitrogen ligase activity, with glutamine as amido-N-donor

GO:0070681; Biological process:glutaminyl-tRNAGln biosynthesis via transamidation

GO:0032543; Biological process:mitochondrial translation

Cross-references [?]

General

TransitM; -; 0-1;

Features [?]

Additional information [?]