HAMAP rule MF_03170

General rule information [?]

Accession	MF_03170
Dates	11-MAR-2013 (Created) 1-JUN-2023 (Last updated, Version 14)

Name	Adenylate_kinase_AK4

Scope

Eukaryota; Vertebrata

Template

P27144 (KAD4_HUMAN)

case <OC:Eukaryota>

Triggered by

HAMAP; MF_03170 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

KAD4

Protein name

RecName:		Full=Adenylate kinase 4, mitochondrial;
		Short=AK 4;
		EC 2.7.4.10;
		EC 2.7.4.6;
AltName:		Full=Adenylate kinase 3-like;
AltName:		Full=GTP:AMP phosphotransferase AK4;

Gene name

AK4, AK3L1

Comments [?]

Function	Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.
Catalytic activity	RHEA:13749: a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'-diphosphate + ADP EC 2.7.4.10
	RHEA:44640: a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP EC 2.7.4.6
	RHEA:18113: a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP EC 2.7.4.6
Subunit	Monomer.
Subcellular location	Mitochondrion matrix.
Domain	Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP/ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP/ATP hydrolysis.
Similarity	Belongs to the adenylate kinase family. AK3 subfamily.

Keywords [?]

ATP-binding
GTP-binding
Kinase
Mitochondrion
Nucleotide-binding
Transferase

Gene Ontology [?]

GO:0005759; Cellular component: mitochondrial matrix.
GO:0046899; Molecular function: nucleoside triphosphate adenylate kinase activity.
GO:0006172; Biological process: ADP biosynthetic process.
GO:0046033; Biological process: AMP metabolic process.
GO:0046039; Biological process: GTP metabolic process.
GO:0046034; Biological process: ATP metabolic process.

Cross-references [?]

PROSITE	PS00113; ADENYLATE_KINASE; 1;
Pfam	PF00406; ADK; 1;
	PF05191; ADK_lid; 1;
PRINTS	PR00094; ADENYLTKNASE; 1;
NCBIfam	TIGR01351; adk; 1;

Features [?]

From: KAD4_HUMAN (P27144)

Key From To Description Tag Condition FTGroup

BINDING 15 20 /ligand="a ribonucleoside 5'-triphosphate" /ligand_id="ChEBI:CHEBI:61557 G-x-G-K-G-T

BINDING 62 64 /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215 x-L-V

BINDING 89 92 /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215 G-F-P-R

BINDING 135 136 /ligand="a ribonucleoside 5'-triphosphate" /ligand_id="ChEBI:CHEBI:61557 V-Y

REGION 35 64 NMPbind

REGION 125 162 LID

BINDING 36 36 /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215 S

BINDING 41 41 /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215 R

BINDING 96 96 /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215 Q

BINDING 126 126 /ligand="a ribonucleoside 5'-triphosphate" /ligand_id="ChEBI:CHEBI:61557 R

BINDING 170 170 /ligand="AMP" /ligand_id="ChEBI:CHEBI:456215 R

BINDING 199 199 /ligand="a ribonucleoside 5'-triphosphate" /ligand_id="ChEBI:CHEBI:61557 T

Additional information [?]

Size range	223-231 amino acids
Related rules	MF_00235 (KAD); MF_03168 (KAD2); MF_03169 (KAD3); MF_03171 (KAD1); MF_03172 (KCY)
Fusion	None

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