HAMAP rule MF_03175
General rule information
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| Accession | MF_03175 |
| Dates | 5-JUN-2013 (Created)
7-DEC-2023 (Last updated, Version 12) |
| Name | MetAP_2_euk |
| Scope(s) |
Eukaryota |
| Template(s) | P50579 (MAP2_HUMAN); P38062 (MAP2_RAT); P38174 (MAP2_YEAST); Q8SR45 (MAP2_ENCCU); [ Recover all ] |
| Triggered by |
HAMAP; MF_03175 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MAP2 |
| Protein name | RecName: Full=Methionine aminopeptidase 2; Short=MAP 2; Short=MetAP 2; EC=3.4.11.18; AltName: Full=Peptidase M; |
| case <OC:Vertebrata> | |
| Protein name | + AltName: Full=Initiation factor 2-associated 67 kDa glycoprotein; Short=p67eIF2; Short=p67; |
| end case | |
| case <OC:Vertebrata> | |
| Gene name | Name=METAP2; |
| else case <OC:Saccharomycotina> | |
| Gene name | Name=MAP2; |
| end case | |
Comments
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| FUNCTION | Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met- Ala-, Cys, Gly, Pro, Ser, Thr, or Val). |
| case <OC:Vertebrata> | |
| FUNCTION | Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. |
| SUBUNIT | Binds EIF2S1 at low magnesium concentrations. Interacts strongly with the eIF-2 gamma-subunit EIF2S3. |
| PTM | Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.; EC=3.4.11.18; |
| COFACTOR | Name=Co(2+); Xref=ChEBI:CHEBI:48828; Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.; |
| case <OC:Vertebrata> | |
| SUBCELLULAR LOCATION | Cytoplasm. Note=About 30% of expressed @gn(METAP2) associates with polysomes. |
| else | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| end case | |
| SIMILARITY | Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily. |
Keywords
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| Aminopeptidase | |
| Cytoplasm | |
| Hydrolase | |
| Metal-binding | |
| Protease | |
| case <OC:Vertebrata> | |
| Glycoprotein | |
| end case | |
Gene Ontology
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| GO:0046872; Molecular function:metal ion binding |
| GO:0070006; Molecular function:metalloaminopeptidase activity |
| GO:0004239; Molecular function:initiator methionyl aminopeptidase activity |
Cross-references
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| Pfam | PF00557; Peptidase_M24; 1; |
| PRINTS | PR00599; MAPEPTIDASE; 1; |
| NCBIfam | TIGR00501; met_pdase_II; 1; |
| PROSITE | PS01202; MAP_2; 1; |
| PROSITE | PS50318; LYS_RICH; 0-unlimited; |
| General | POLY_LYS; PolyAA; 0-unlimited; |
Features
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| From: MAP2_HUMAN (P50579) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 251 | 251 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1" |
D | ||||||||
| BINDING | 262 | 262 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1" |
D | ||||||||
| BINDING | 262 | 262 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic" |
D | ||||||||
| BINDING | 331 | 331 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic" |
H | ||||||||
| BINDING | 364 | 364 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic" |
E | ||||||||
| BINDING | 459 | 459 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1" |
E | ||||||||
| BINDING | 459 | 459 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic" |
E | ||||||||
| BINDING | 231 | 231 | /ligand="substrate" | H | ||||||||
| BINDING | 339 | 339 | /ligand="substrate" | H | ||||||||
Additional information
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| Size range | 357-486 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |