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HAMAP rule MF_03180

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General rule information [?]

Accession MF_03180
Dates 17-OCT-2013 (Created)
3-SEP-2024 (Last updated, Version 18)
Name GCP2_Kae1
Scope(s) Eukaryota
Template(s) Q9UXT7 (KAE1_PYRAB); P36132 (KAE1_YEAST); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_01446 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Fungi>
Identifier KAE1
Protein name RecName: Full=tRNA N6-adenosine threonylcarbamoyltransferase;
                 EC=2.3.1.234;
AltName: Full=N6-L-threonylcarbamoyladenine synthase;
                 Short=t(6)A synthase;
AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein ;
AltName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein ;
else case <OC:Vertebrata>
Protein name RecName: Full=tRNA N6-adenosine threonylcarbamoyltransferase;
                 EC=2.3.1.234;
AltName: Full=N6-L-threonylcarbamoyladenine synthase;
                 Short=t(6)A synthase;
AltName: Full=O-sialoglycoprotein endopeptidase;
AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein ;
AltName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein ;
else
Identifier OSGEP
Protein name RecName: Full=Probable tRNA N6-adenosine threonylcarbamoyltransferase;
                 EC=2.3.1.234;
AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein ;
AltName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein ;
end case
case <OC:Viridiplantae>
Protein name + AltName: Full=Glycoprotease 2;
Gene name Name=GCP2;
else case <OC:Dictyostelia>
Gene name Name=osgep;
else case <OC:Fungi>
Gene name Name=KAE1;
end case

Comments [?]

case <OC:Fungi>
FUNCTIONComponent of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. @gn(KAE1) likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.
SUBUNITComponent of the EKC/KEOPS complex composed of at least @gn(BUD32), @gn(CGI121), @gn(GON7), @gn(KAE1) and @gn(PCC1); the whole complex dimerizes.
else case <OC:Vertebrata>
FUNCTIONComponent of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. @gn(OSGEP) likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.
SUBUNITComponent of the EKC/KEOPS complex composed of at least @gn(TP53RK), @gn(TPRKB), @gn(OSGEP) and @gn(LAGE3); the whole complex dimerizes.
else
FUNCTIONComponent of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.
SUBUNITComponent of the EKC/KEOPS complex; the whole complex dimerizes.
end case
CATALYTIC ACTIVITY Reaction=L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L- threonylcarbamoyladenosine(37) in tRNA + AMP + H(+); Xref=Rhea:RHEA:37059, Rhea:RHEA-COMP:10162, Rhea:RHEA-COMP:10163, ChEBI:CHEBI:15378, ChEBI:CHEBI:73682, ChEBI:CHEBI:74411, ChEBI:CHEBI:74418, ChEBI:CHEBI:456215; EC=2.3.1.234;
COFACTOR Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Note=Binds 1 divalent metal cation per subunit.;
SUBCELLULAR LOCATIONCytoplasm. Nucleus.
SIMILARITYBelongs to the KAE1 / TsaD family.

Keywords [?]

Acyltransferase
Cytoplasm
Metal-binding
Nucleus
Transferase
tRNA processing
case <OC:Fungi>
Activator
Transcription
Transcription regulation
end case

Gene Ontology [?]

GO:0005737; Cellular component:cytoplasm
GO:0016747; Molecular function:acyltransferase activity, transferring groups other than amino-acyl groups
GO:0002949; Biological process:tRNA threonylcarbamoyladenosine modification

Cross-references [?]

PROSITE PS01016; GLYCOPROTEASE; 1;
Pfam PF00814; Peptidase_M22; 1;
PRINTS PR00789; OSIALOPTASE; 1;
NCBIfam TIGR00329; Gcp_kae1; 1;

Features [?]

From: KAE1_PYRAB (Q9UXT7)
Key From To Description Tag Condition FTGroup
BINDING 127 131 /ligand="substrate" x-x-[SA]-G-[GA]
BINDING 107 107 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 H
BINDING 111 111 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 H
BINDING 127 127 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 Y
BINDING 285 285 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 D
BINDING 159 159 /ligand="substrate" D
BINDING 172 172 /ligand="substrate" [GA]
BINDING 176 176 /ligand="substrate" [ED]
BINDING 257 257 /ligand="substrate" [NS]

Additional information [?]

Size range 325-407 amino acids
Related rules MF_01447
Fusion Nter: None Cter: None
Comments Was originally (PubMed:17766251) thought to have endonuclease activity, but it could not be confirmed with orthologs purified from M. jannaschii (PubMed:18951093) and S. cerevisiae (PubMed:21183954).



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