HAMAP rule MF_03180
General rule information
[?]
Accession | MF_03180 |
Dates | 17-OCT-2013 (Created) 19-NOV-2022 (Last updated, Version 16) |
Name | GCP2_Kae1 |
Scope | Eukaryota |
Templates | Q9UXT7 (KAE1_PYRAB); P36132 (KAE1_YEAST): [Recover all] |
case <OC:Eukaryota>
Triggered by |
end case
Propagated annotation
[?]
Identifier, protein and gene names
[?]
case <OC:Fungi>
Identifier |
|
Protein name |
|
else case <OC:Vertebrata>
Protein name |
|
else
Identifier |
|
Protein name |
|
end case
case <OC:Viridiplantae>
Protein name |
|
Gene name |
|
else case <OC:Dictyostelia>
Gene name |
|
else case <OC:Fungi>
Gene name |
|
end case
Comments
[?]
case <OC:Fungi>
Function | Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. @gn(KAE1) likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. |
Subunit | Component of the EKC/KEOPS complex composed of at least @gn(BUD32), @gn(CGI121), @gn(GON7), @gn(KAE1) and @gn(PCC1); the whole complex dimerizes. |
else case <OC:Vertebrata>
Function | Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. @gn(OSGEP) likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. |
Subunit | Component of the EKC/KEOPS complex composed of at least @gn(TP53RK), @gn(TPRKB), @gn(OSGEP) and @gn(LAGE3); the whole complex dimerizes. |
else
Function | Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. |
Subunit | Component of the EKC/KEOPS complex; the whole complex dimerizes. |
end case
Catalytic activity | RHEA:37059: adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA
EC 2.3.1.234 |
Cofactor | a divalent metal cation Note: Binds 1 divalent metal cation per subunit. |
Subcellular location | Cytoplasm. Nucleus. |
Similarity | Belongs to the KAE1 / TsaD family. |
Keywords
[?]
case <OC:Fungi>
end case
Gene Ontology
[?]
GO:0005737; Cellular component: cytoplasm.
GO:0016747; Molecular function: acyltransferase activity, transferring groups other than amino-acyl groups.
GO:0002949; Biological process: tRNA threonylcarbamoyladenosine modification.
GO:0016747; Molecular function: acyltransferase activity, transferring groups other than amino-acyl groups.
GO:0002949; Biological process: tRNA threonylcarbamoyladenosine modification.
Cross-references
[?]
PROSITE | PS01016; GLYCOPROTEASE; 1; |
Pfam | PF00814; Peptidase_M22; 1; |
PRINTS | PR00789; OSIALOPTASE; 1; |
TIGRFAMs | TIGR00329; Gcp_kae1; 1; |
Features
[?]
From: KAE1_PYRAB (Q9UXT7) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 127 | 131 | /ligand="substrate | x-x-[SA]-G-[GA] | ||||||||
BINDING | 107 | 107 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240 | H | ||||||||
BINDING | 111 | 111 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240 | H | ||||||||
BINDING (Optional) | 127 | 127 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240 | Y | ||||||||
BINDING | 285 | 285 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240 | D | ||||||||
BINDING | 159 | 159 | /ligand="substrate | D | ||||||||
BINDING (Optional) | 172 | 172 | /ligand="substrate | [GA] | ||||||||
BINDING | 176 | 176 | /ligand="substrate | [ED] | ||||||||
BINDING | 257 | 257 | /ligand="substrate | [NS] |
Additional information
[?]
Size range | 325-407 amino acids |
Related rules | MF_01447 (KAE1B supersedes the current rule) |
Fusion | None |
Comments | Was originally (PubMed:17766251) thought to have endonuclease activity, but it could not be confirmed with orthologs purified from M. jannaschii (PubMed:18951093) and S. cerevisiae (PubMed:21183954). |