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Annotation rule MF_03186 |
General rule information
[?]
Accession |
MF_03186 |
Dates |
16-MAY-2014 (Created) 11-FEB-2020 (Last updated, Version 9) |
Name |
Phosphofructokinase_II_X_euk |
case <OC:Eukaryota>
end case
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=ATP-dependent 6-phosphofructokinase;
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Short=ATP-PFK;
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Short=Phosphofructokinase;
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EC 2.7.1.11;
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AltName:
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Full=Phosphohexokinase;
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case <OC:Viridiplantae>
else case <OC:Kinetoplastea>
end case
Function |
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. |
Catalytic activity |
RHEA:16109: ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)
EC 2.7.1.11
|
Cofactor |
Mg(2+) |
Activity regulation |
Allosterically activated by AMP. |
Pathway |
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. |
Subunit |
Homotetramer. |
case <OC:Kinetoplastea>
Subcellular location |
Glycosome. |
else
Subcellular location |
Cytoplasm. |
end case
Similarity |
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily. |
case <OC:Kinetoplastea>
else
end case
GO:0003872; Molecular function: 6-phosphofructokinase activity.
GO:0006096; Biological process: glycolytic process.
GO:0005737; Cellular component: cytoplasm.
From: PFKA_TRYBB (O15648) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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SITE (Optional)
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200
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200
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Important for substrate specificity; cannot use PPi as phosphoryl donor
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|
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G
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NP_BIND
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173
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174
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ATP
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[RK]-x
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NP_BIND
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198
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201
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ATP
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G-[DENG]-G-[ST]
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BINDING
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107
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107
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ATP; via amide nitrogen
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G
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REGION
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227
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229
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Substrate binding
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|
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T-x-D
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REGION
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272
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274
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Substrate binding
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|
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M-G-[RH]
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REGION
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380
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383
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Substrate binding
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[HY]-x(2)-R
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ACT_SITE
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229
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229
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Proton acceptor
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D
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METAL
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199
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199
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Magnesium; catalytic
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[DEN]
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BINDING
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325
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325
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Substrate
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E
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case <OC:Kinetoplastea>
MOTIF (Optional)
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Cter-2
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Cter
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Peroxisomal targeting signal
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[AS]-K-[LV]>
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end case
Additional information
[?]
Size range |
436-537 amino acids |
Related rules |
MF_01981 (PFKA) |
Fusion |
None |
Comments |
Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511). |