HAMAP logo

HAMAP rule MF_03186

Send feedback

General rule information [?]

Accession MF_03186
Dates 16-MAY-2014 (Created)
19-NOV-2022 (Last updated, Version 12)
Name Phosphofructokinase_II_X_euk
Scope(s) Eukaryota
Template(s) O15648 (PFKA_TRYBB); Q27651 (PFKA_ENTH1); Q9BIC6 (PFKA_LEIDO); Q4E657 (PFKA_TRYCC); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_01981 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PFKA
Protein name RecName: Full=ATP-dependent 6-phosphofructokinase;
AltName: Full=Phosphohexokinase;
case <OC:Viridiplantae>
Gene name Name=PFK;
else case <OC:Kinetoplastea>
Gene name Name=pfk;
end case

Comments [?]

FUNCTIONCatalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
CATALYTIC ACTIVITY Reaction=ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6- bisphosphate + H(+); Xref=Rhea:RHEA:16109, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32966, ChEBI:CHEBI:57634, ChEBI:CHEBI:456216; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
ACTIVITY REGULATIONAllosterically activated by AMP.
PATHWAYCarbohydrate degradation; glycolysis; D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4.
case <OC:Kinetoplastea>
end case
SIMILARITYBelongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003872; Molecular function:6-phosphofructokinase activity
GO:0006096; Biological process:glycolytic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00365; PFK; 1;
PIRSF PIRSF000534; PPi_PFK_TP0108; 1;

Features [?]

From: PFKA_TRYBB (O15648)
Key From To Description Tag Condition FTGroup
SITE 200 200 /note="Important for substrate specificity; cannot use PPi as phosphoryl donor" G
BINDING 173 174 /ligand="ATP"
BINDING 198 201 /ligand="ATP"
BINDING 107 107 /ligand="ATP"
BINDING 227 229 /ligand="substrate" T-x-D
BINDING 272 274 /ligand="substrate" M-G-[RH]
BINDING 380 383 /ligand="substrate" [HY]-x(2)-R
ACT_SITE 229 229 /note="Proton acceptor" D
BINDING 199 199 /ligand="Mg(2+)"
BINDING 325 325 /ligand="substrate" E
case <OC:Kinetoplastea>
MOTIF Cter-2 Cter /note="Peroxisomal targeting signal" [AS]-K-[LV]>
end case

Additional information [?]

Size range 436-537 amino acids
Related rules MF_01981
Fusion Nter: None Cter: None
Comments Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511).

View rule in raw text format (no links)