HAMAP rule MF_03186
General rule information
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| Accession | MF_03186 |
| Dates | 16-MAY-2014 (Created)
19-NOV-2022 (Last updated, Version 12) |
| Name | Phosphofructokinase_II_X_euk |
| Scope(s) |
Eukaryota |
| Template(s) | O15648 (PFKA_TRYBB); Q27651 (PFKA_ENTH1); Q9BIC6 (PFKA_LEIDO); Q4E657 (PFKA_TRYCC); [ Recover all ] |
| Triggered by |
case c? <OC:Eukaryota>
HAMAP; MF_01981 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PFKA |
| Protein name | RecName: Full=ATP-dependent 6-phosphofructokinase; Short=ATP-PFK; Short=Phosphofructokinase; EC=2.7.1.11; AltName: Full=Phosphohexokinase; |
| case <OC:Viridiplantae> | |
| Gene name | Name=PFK; |
| else case <OC:Kinetoplastea> | |
| Gene name | Name=pfk; |
| end case | |
Comments
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| FUNCTION | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. |
| CATALYTIC ACTIVITY | Reaction=ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6- bisphosphate + H(+); Xref=Rhea:RHEA:16109, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32966, ChEBI:CHEBI:57634, ChEBI:CHEBI:456216; EC=2.7.1.11; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| ACTIVITY REGULATION | Allosterically activated by AMP. |
| PATHWAY | Carbohydrate degradation; glycolysis; D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4. |
| SUBUNIT | Homotetramer. |
| case <OC:Kinetoplastea> | |
| SUBCELLULAR LOCATION | Glycosome. |
| else | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| end case | |
| SIMILARITY | Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily. |
Keywords
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| Allosteric enzyme | |
| Kinase | |
| Transferase | |
| Glycolysis | |
| ATP-binding | |
| Nucleotide-binding | |
| Magnesium | |
| Metal-binding | |
| case <OC:Kinetoplastea> | |
| Glycosome | |
| Peroxisome | |
| else | |
| Cytoplasm | |
| end case | |
Gene Ontology
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| GO:0003872; Molecular function:6-phosphofructokinase activity |
| GO:0006096; Biological process:glycolytic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00365; PFK; 1; |
| PIRSF | PIRSF000534; PPi_PFK_TP0108; 1; |
| PRINTS | PR00476; PHFRCTKINASE; 1; |
Features
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| From: PFKA_TRYBB (O15648) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| SITE | 200 | 200 | /note="Important for substrate specificity; cannot use PPi as phosphoryl donor" | G | ||||||||
| BINDING | 173 | 174 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[RK]-x | ||||||||
| BINDING | 198 | 201 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-[DENG]-G-[ST] | ||||||||
| BINDING | 107 | 107 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
| BINDING | 227 | 229 | /ligand="substrate" | T-x-D | ||||||||
| BINDING | 272 | 274 | /ligand="substrate" | M-G-[RH] | ||||||||
| BINDING | 380 | 383 | /ligand="substrate" | [HY]-x(2)-R | ||||||||
| ACT_SITE | 229 | 229 | /note="Proton acceptor" | D | ||||||||
| BINDING | 199 | 199 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="catalytic" |
[DEN] | ||||||||
| BINDING | 325 | 325 | /ligand="substrate" | E | ||||||||
| case <OC:Kinetoplastea> | ||||||||||||
| MOTIF | Cter-2 | Cter | /note="Peroxisomal targeting signal" | [AS]-K-[LV]> | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 436-537 amino acids |
| Related rules |
MF_01981 |
| Fusion | Nter: None Cter: None |
| Comments | Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511). |