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HAMAP rule MF_03186

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General rule information [?]

Accession MF_03186
Dates 16-MAY-2014 (Created)
19-NOV-2022 (Last updated, Version 12)
Name Phosphofructokinase_II_X_euk
Scope
Eukaryota
Templates O15648 (PFKA_TRYBB); Q27651 (PFKA_ENTH1); Q9BIC6 (PFKA_LEIDO); Q4E657 (PFKA_TRYCC): [Recover all]
case <OC:Eukaryota>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PFKA
Protein name
RecName: Full=ATP-dependent 6-phosphofructokinase;
Short=ATP-PFK;
Short=Phosphofructokinase;
EC 2.7.1.11;
AltName: Full=Phosphohexokinase;
case <OC:Viridiplantae>
Gene name
PFK
else case <OC:Kinetoplastea>
Gene name
pfk
end case

Comments [?]

Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity RHEA:16109: ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)
EC 2.7.1.11
Cofactor Mg(2+)
Activity regulation Allosterically activated by AMP.
Pathway Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit Homotetramer.
case <OC:Kinetoplastea>
Subcellular location Glycosome.
else
Subcellular location Cytoplasm.
end case
Similarity Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.

Keywords [?]

Allosteric enzyme
Kinase
Transferase
Glycolysis
ATP-binding
Nucleotide-binding
Magnesium
Metal-binding
case <OC:Kinetoplastea>
Glycosome
Peroxisome
else
Cytoplasm
end case

Gene Ontology [?]

GO:0003872; Molecular function: 6-phosphofructokinase activity.
GO:0006096; Biological process: glycolytic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00365; PFK; 1;
PIRSF PIRSF000534; PPi_PFK_TP0108; 1;
PRINTS PR00476; PHFRCTKINASE; 1;

Features [?]

From: PFKA_TRYBB (O15648)
Key     From     To       Description   Tag   Condition   FTGroup
SITE (Optional)     200     200       Important for substrate specificity; cannot use PPi as phosphoryl donor     G  
BINDING     173     174       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [RK]-x  
BINDING     198     201       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-[DENG]-G-[ST]  
BINDING     107     107       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G  
BINDING     227     229       /ligand="substrate     T-x-D  
BINDING     272     274       /ligand="substrate     M-G-[RH]  
BINDING     380     383       /ligand="substrate     [HY]-x(2)-R  
ACT_SITE     229     229       Proton acceptor     D  
BINDING     199     199       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="catalytic     [DEN]  
BINDING     325     325       /ligand="substrate     E  
case <OC:Kinetoplastea>
MOTIF (Optional)     Cter-2     Cter       Peroxisomal targeting signal     [AS]-K-[LV]>  
end case

Additional information [?]

Size range 436-537 amino acids
Related rules MF_01981 (PFKA)
Fusion None
Comments Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511).


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