HAMAP rule MF_03195
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_03195 |
| Accession | MF_03195 |
| Dates | 17-JUL-2015 (Created)
3-SEP-2024 (Last updated, Version 12) |
| Name | GMP_reduct_type1_euk |
| Scope(s) |
Eukaryota |
| Template(s) | Q9P2T1 (GMPR2_HUMAN); P36959 (GMPR1_HUMAN); Q4QEB3 (GMPR_LEIMA); [ Recover all ] |
| Triggered by |
case c? <OC:Eukaryota>
HAMAP; MF_00596 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GMPR |
| Protein name | RecName: Full=GMP reductase; Short=GMPR; EC=1.7.1.7; AltName: Full=Guanosine 5'-monophosphate oxidoreductase; Short=Guanosine monophosphate reductase; |
| case <OC:Vertebrata> or <OC:Kinetoplastea> | |
| Gene name | Name=GMPR; |
| end case | |
Comments
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| FUNCTION | Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides. |
| CATALYTIC ACTIVITY | Reaction=IMP + NH4(+) + NADP(+) = GMP + NADPH + 2 H(+); Xref=Rhea:RHEA:17185, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:57783, ChEBI:CHEBI:58053, ChEBI:CHEBI:58115, ChEBI:CHEBI:58349; EC=1.7.1.7; |
| SUBUNIT | Homotetramer. |
| case <OC:Kinetoplastea> and <FTTag:Microbody> | |
| SUBCELLULAR LOCATION | Glycosome. |
| end case | |
| SIMILARITY | Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily. |
Keywords
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| Metal-binding | |
| NADP | |
| Oxidoreductase | |
| Potassium | |
| Purine metabolism | |
| case <OC:Kinetoplastea> and <FTTag:Microbody> | |
| Glycosome | |
| Peroxisome | |
| end case | |
Gene Ontology
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| GO:0003920; Molecular function:GMP reductase activity |
| GO:0006163; Biological process:purine nucleotide metabolic process |
Cross-references
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| PROSITE | PS00487; IMP_DH_GMP_RED; 1; |
| PROSITE | PS51371; CBS; 0-2; |
| Pfam | PF00478; IMPDH; 1; |
| Pfam | PF00571; CBS; 0-2; |
| NCBIfam | TIGR01305; GMP_reduct_1; 1; |
| PIRSF | PIRSF000235; GMP_reductase; 1; |
Features
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| From: GMPR2_HUMAN (Q9P2T1) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 26 | 27 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" /ligand_note="ligand shared between two neighboring subunits" |
S-R | ||||||||
| BINDING | 129 | 131 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
D-[IV]-A | ||||||||
| BINDING | 180 | 181 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
[IV]-G | ||||||||
| BINDING | 285 | 286 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
Y-R | ||||||||
| BINDING | 314 | 317 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" /ligand_note="ligand shared between two neighboring subunits" |
S-[ATG]-[CI]-[TS] | ||||||||
| BINDING | 219 | 221 | /ligand="GMP" /ligand_id="ChEBI:CHEBI:58115" |
D-G-G | ||||||||
| BINDING | 242 | 243 | /ligand="GMP" /ligand_id="ChEBI:CHEBI:58115" |
G-[GN] | ||||||||
| BINDING | 268 | 270 | /ligand="GMP" /ligand_id="ChEBI:CHEBI:58115" |
G-M-[AS] | ||||||||
| BINDING | 286 | 290 | /ligand="GMP" /ligand_id="ChEBI:CHEBI:58115" |
[RL]-[AV]-[SAP]-E-G | ||||||||
| ACT_SITE | 186 | 186 | /note="Thioimidate intermediate" | C | ||||||||
| ACT_SITE | 188 | 188 | /note="Proton donor/acceptor" | T | ||||||||
| BINDING | 181 | 181 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103" |
G | ||||||||
| BINDING | 183 | 183 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103" |
G | ||||||||
| BINDING | 186 | 186 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103" |
C | ||||||||
| BINDING | 189 | 189 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103" |
R | ||||||||
| BINDING | 78 | 78 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
[KR] | ||||||||
| BINDING | 269 | 269 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
M | ||||||||
| From: GMPR_LEIMA (Q4QEB3) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Kinetoplastea> | ||||||||||||
| MOTIF | 490 | Cter | /note="Microbody targeting signal" | Microbody | [AS]-K-[ML] | |||||||
| end case | ||||||||||||
Additional information
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| Size range | 339-553 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |