HAMAP rule MF_03215

General rule information [?]

Accession	MF_03215
Dates	29-FEB-2016 (Created) 19-NOV-2022 (Last updated, Version 8)

Name	Ribokinase_euk

Scope

Eukaryota

Templates

Q9H477 (RBSK_HUMAN); P0A9J6 (RBSK_ECOLI): [Recover all]

case <OC:Eukaryota>

Triggered by

HAMAP; MF_01987 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

RBSK

Protein name

RecName:		Full=Ribokinase;
		Short=RK;
		EC 2.7.1.15;

case <OC:Vertebrata>

Gene name

RBKS

else case <OC:Saccharomycotina>

Gene name

RBK1

end case

Comments [?]

Function	Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.
Catalytic activity	RHEA:13697: ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+) EC 2.7.1.15
Cofactor	Mg(2+) Note: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.
Activity regulation	Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.
Pathway	Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.
Subunit	Homodimer.
Subcellular location	Cytoplasm. Nucleus.
Similarity	Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.

Keywords [?]

ATP-binding
Carbohydrate metabolism
Cytoplasm
Kinase
Magnesium
Metal-binding
Nucleotide-binding
Nucleus
Potassium
Transferase

Gene Ontology [?]

GO:0005737; Cellular component: cytoplasm.
GO:0005634; Cellular component: nucleus.
GO:0005524; Molecular function: ATP binding.
GO:0004747; Molecular function: ribokinase activity.
GO:0046835; Biological process: carbohydrate phosphorylation.
GO:0019303; Biological process: D-ribose catabolic process.

Cross-references [?]

PROSITE	PS00584; PFKB_KINASES_2; 1;
Pfam	PF00294; PfkB; 1;
PRINTS	PR00990; RIBOKINASE; 1;
TIGRFAMs	TIGR02152; D_ribokin_bact; 1;

Features [?]

From: RBSK_HUMAN (Q9H477)

Key From To Description Tag Condition FTGroup

BINDING 235 240 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 x(2)-G-x(2)-G

BINDING 268 269 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G-D

BINDING 25 27 /ligand="substrate x(2)-D

BINDING 53 57 /ligand="substrate G-[KR]-[GAS]-x-[NR]

ACT_SITE 269 269 Proton acceptor D

BINDING (Optional) 263 263 /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103 [DN]

BINDING 265 265 /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103

BINDING 301 301 /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103

BINDING 304 304 /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103

BINDING (Optional) 306 306 /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103 G

BINDING (Optional) 310 310 /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103 S

BINDING (Optional) 154 154 /ligand="substrate E

BINDING 199 199 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 N

BINDING (Optional) 256 256 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 T

BINDING 269 269 /ligand="substrate D

BINDING (Optional) 295 295 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [HN]

Additional information [?]

Size range	280-470 amino acids
Related rules	None
Fusion	None

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