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HAMAP rule MF_03215

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General rule information [?]

Accession MF_03215
Dates 29-FEB-2016 (Created)
19-NOV-2022 (Last updated, Version 8)
Name Ribokinase_euk
Scope
Eukaryota
Templates Q9H477 (RBSK_HUMAN); P0A9J6 (RBSK_ECOLI): [Recover all]
case <OC:Eukaryota>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RBSK
Protein name
RecName: Full=Ribokinase;
Short=RK;
EC 2.7.1.15;
case <OC:Vertebrata>
Gene name
RBKS
else case <OC:Saccharomycotina>
Gene name
RBK1
end case

Comments [?]

Function Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.
Catalytic activity RHEA:13697: ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+)
EC 2.7.1.15
Cofactor Mg(2+)
Note: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.
Activity regulation Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.
Pathway Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.
Subunit Homodimer.
Subcellular location Cytoplasm. Nucleus.
Similarity Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005737; Cellular component: cytoplasm.
GO:0005634; Cellular component: nucleus.
GO:0005524; Molecular function: ATP binding.
GO:0004747; Molecular function: ribokinase activity.
GO:0046835; Biological process: carbohydrate phosphorylation.
GO:0019303; Biological process: D-ribose catabolic process.

Cross-references [?]

PROSITE PS00584; PFKB_KINASES_2; 1;
Pfam PF00294; PfkB; 1;
PRINTS PR00990; RIBOKINASE; 1;
TIGRFAMs TIGR02152; D_ribokin_bact; 1;

Features [?]

From: RBSK_HUMAN (Q9H477)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     235     240       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     x(2)-G-x(2)-G  
BINDING     268     269       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-D  
BINDING     25     27       /ligand="substrate     x(2)-D  
BINDING     53     57       /ligand="substrate     G-[KR]-[GAS]-x-[NR]  
ACT_SITE     269     269       Proton acceptor     D  
BINDING (Optional)     263     263       /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103     [DN]  
BINDING     265     265       /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103        
BINDING     301     301       /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103        
BINDING     304     304       /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103        
BINDING (Optional)     306     306       /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103     G  
BINDING (Optional)     310     310       /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103     S  
BINDING (Optional)     154     154       /ligand="substrate     E  
BINDING     199     199       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     N  
BINDING (Optional)     256     256       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     T  
BINDING     269     269       /ligand="substrate     D  
BINDING (Optional)     295     295       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [HN]  

Additional information [?]

Size range 280-470 amino acids
Related rules None
Fusion None