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Annotation rule MF_03215
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General rule information [?]

Accession MF_03215
Dates 29-FEB-2016 (Created)
20-NOV-2019 (Last updated, Version 5)
Name Ribokinase_euk
Templates Q9H477 (RBSK_HUMAN); P0A9J6 (RBSK_ECOLI): [Recover all]
case <OC:Eukaryota>
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Ribokinase;
case <OC:Vertebrata>
Gene name
else case <OC:Saccharomycotina>
Gene name
end case

Comments [?]

Function Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.
Catalytic activity RHEA:13697: ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+)
Cofactor Mg(2+)
Note: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.
Activity regulation Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.
Pathway Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.
Subunit Homodimer.
Subcellular location Cytoplasm. Nucleus.
Similarity Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.

Keywords [?]

Gene Ontology [?]

GO:0005737; Cellular component: cytoplasm.
GO:0005634; Cellular component: nucleus.
GO:0005524; Molecular function: ATP binding.
GO:0004747; Molecular function: ribokinase activity.
GO:0046835; Biological process: carbohydrate phosphorylation.
GO:0019303; Biological process: D-ribose catabolic process.

Cross-references [?]

Pfam PF00294; PfkB; 1;
TIGRFAMs TIGR02152; D_ribokin_bact; 1;

Features [?]

From: RBSK_HUMAN (Q9H477)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     235     240       ATP     x(2)-G-x(2)-G  
NP_BIND     268     269       ATP     G-D  
REGION     25     27       Substrate binding     x(2)-D  
REGION     53     57       Substrate binding     G-[KR]-[GAS]-x-[NR]  
ACT_SITE     269     269       Proton acceptor     D  
METAL (Optional)     263     263       Potassium     [DN]  
METAL     265     265       Potassium; via carbonyl oxygen        
METAL     301     301       Potassium; via carbonyl oxygen        
METAL     304     304       Potassium; via carbonyl oxygen        
METAL (Optional)     306     306       Potassium; via carbonyl oxygen     G  
METAL (Optional)     310     310       Potassium     S  
BINDING (Optional)     154     154       Substrate     E  
BINDING     199     199       ATP     N  
BINDING (Optional)     256     256       ATP     T  
BINDING     269     269       Substrate     D  
BINDING (Optional)     295     295       ATP     [HN]  

Additional information [?]

Size range 280-470 amino acids
Related rules None
Fusion None