HAMAP rule MF_03218
General rule information
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Accession | MF_03218 |
Dates | 22-JUL-2016 (Created) 1-JUN-2023 (Last updated, Version 12) |
Name | Q_tRNA_Tgt_euk |
Scope | Eukaryota |
Templates | Q9BXR0 (TGT_HUMAN); P28720 (TGT_ZYMMO); P0A847 (TGT_ECOLI); Q9JMA2 (TGT_MOUSE): [Recover all] |
case <OC:Eukaryota>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
Protein name |
|
case <OC:Vertebrata>
Gene name |
|
else case <OC:Caenorhabditis>
Gene name |
|
end case
Comments
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Function | Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product. |
Catalytic activity | RHEA:16633: guanosine(34) in tRNA + queuine = guanine + queuosine(34) in tRNA
EC 2.4.2.64 |
case <FTGroup:1>
Cofactor | Zn(2+) |
end case
case <OC:Vertebrata>
Subunit | Heterodimer of a catalytic subunit @gn(QTRT1) and an accessory subunit @gn(QTRT2). |
Subcellular location | Cytoplasm. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note=Weakly associates with mitochondria, possibly via @gn(QTRT2). |
else
Subunit | Heterodimer of a catalytic subunit and an accessory subunit. |
Subcellular location | Cytoplasm. |
end case
Similarity | Belongs to the queuine tRNA-ribosyltransferase family. |
Keywords
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case <OC:Vertebrata>
end case
case <FTGroup:1>
end case
Gene Ontology
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GO:0005737; Cellular component: cytoplasm.
case <OC:Vertebrata>
GO:0005741; Cellular component: mitochondrial outer membrane.
end case
GO:0008479; Molecular function: queuine tRNA-ribosyltransferase activity.
GO:0101030; Biological process: tRNA-guanine transglycosylation.
GO:0101030; Biological process: tRNA-guanine transglycosylation.
Cross-references
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Features
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From: TGT_ZYMMO (P28720) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 102 | 106 | /ligand="substrate | D-S-G-G-[FY] | ||||||||
REGION | 261 | 267 | RNA binding | G-[VIG]-G-x(4) | ||||||||
REGION | 285 | 289 | RNA binding; important for wobble base 34 recognition | [TA]-[RK]-x(2)-R | ||||||||
ACT_SITE | 102 | 102 | Proton acceptor | D | ||||||||
ACT_SITE | 280 | 280 | Nucleophile | D | ||||||||
BINDING | 318 | 318 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | 1 | |||||||
BINDING | 320 | 320 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | 1 | |||||||
BINDING | 323 | 323 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | 1 | |||||||
BINDING | 349 | 349 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | H | 1 | |||||||
BINDING | 156 | 156 | /ligand="substrate | D | ||||||||
BINDING | 203 | 203 | /ligand="substrate | Q | ||||||||
BINDING | 230 | 230 | /ligand="substrate | G |
Additional information
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Size range | 396-427 amino acids |
Related rules | None |
Fusion | None |