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Annotation rule MF_03218 |
General rule information
[?]
Accession |
MF_03218 |
Dates |
22-JUL-2016 (Created) 20-NOV-2019 (Last updated, Version 6) |
case <OC:Eukaryota>
end case
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=Queuine tRNA-ribosyltransferase catalytic subunit 1;
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EC 2.4.2.29;
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AltName:
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Full=Guanine insertion enzyme;
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AltName:
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Full=tRNA-guanine transglycosylase;
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case <OC:Vertebrata>
else case <OC:Caenorhabditis>
end case
Function |
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product. |
Catalytic activity |
RHEA:16633: guanosine(34) in tRNA + queuine = guanine + queuosine(34) in tRNA
EC 2.4.2.29
|
case <FTGroup:1>
end case
case <OC:Vertebrata>
Subunit |
Heterodimer of a catalytic subunit @gn(QTRT1) and an accessory subunit @gn(QTRT2). |
Subcellular location |
Cytoplasm. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note=Weakly associates with mitochondria, possibly via @gn(QTRT2). |
else
Subunit |
Heterodimer of a catalytic subunit and an accessory subunit. |
Subcellular location |
Cytoplasm. |
end case
Similarity |
Belongs to the queuine tRNA-ribosyltransferase family. |
case <OC:Vertebrata>
end case
case <FTGroup:1>
end case
case <OC:Vertebrata>
GO:0005741; Cellular component: mitochondrial outer membrane.
end case
GO:0008479; Molecular function: queuine tRNA-ribosyltransferase activity.
GO:0101030; Biological process: tRNA-guanine transglycosylation.
From: TGT_ZYMMO (P28720) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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REGION
|
|
102
|
|
106
|
|
Substrate binding
|
|
|
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D-S-G-G-[FY]
|
|
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REGION
|
|
261
|
|
267
|
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RNA binding
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G-[VIG]-G-x(4)
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REGION
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285
|
|
289
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RNA binding; important for wobble base 34 recognition
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[TA]-[RK]-x(2)-R
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ACT_SITE
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102
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|
102
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Proton acceptor
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|
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D
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ACT_SITE
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280
|
|
280
|
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Nucleophile
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|
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D
|
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METAL
|
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318
|
|
318
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Zinc
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|
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C
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1
|
METAL
|
|
320
|
|
320
|
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Zinc
|
|
|
|
C
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|
1
|
METAL
|
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323
|
|
323
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Zinc
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|
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C
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1
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METAL
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349
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349
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Zinc; via pros nitrogen
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H
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1
|
BINDING
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156
|
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156
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Substrate
|
|
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D
|
|
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BINDING
|
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203
|
|
203
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Substrate
|
|
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Q
|
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BINDING
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230
|
|
230
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Substrate; via amide nitrogen
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G
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Additional information
[?]
Size range |
396-427 amino acids |
Related rules |
None |
Fusion |
None |