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HAMAP rule MF_03220

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General rule information [?]

Accession MF_03220
Dates 20-OCT-2016 (Created)
19-NOV-2022 (Last updated, Version 8)
Name Succ_CoA_betaA_euk
Templates Q9YI37 (SUCB1_COLLI); P0A836 (SUCC_ECOLI); P53590 (SUCB2_PIG): [Recover all]
case <OC:Metazoa> or <OC:Amoebozoa>
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial;
AltName: Full=ATP-specific succinyl-CoA synthetase subunit beta;
AltName: Full=Succinyl-CoA synthetase beta-A chain;
case <OC:Vertebrata>
Gene name
end case

Comments [?]

Function ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Catalytic activity RHEA:17661: ATP + CoA + succinate = ADP + phosphate + succinyl-CoA
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
Pathway Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Subunit Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for ATP.
Subcellular location Mitochondrion.
Similarity Belongs to the succinate/malate CoA ligase beta subunit family. ATP-specific subunit beta subfamily.
case not <AnyFeature:TransitM>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0004775; Molecular function: succinate-CoA ligase (ADP-forming) activity.
GO:0006099; Biological process: tricarboxylic acid cycle.

Cross-references [?]

Pfam PF08442; ATP-grasp_2; 1;
PF00549; Ligase_CoA; 1;
TIGRFAMs TIGR01016; sucCoAbeta; 1;
PIRSF PIRSF001554; SucCS_beta; 1;

Computed features [?]

General TransitM; -; 0-1; trigger=yes;

Features [?]

case <Feature:PS50975>
From: SUCB1_COLLI (Q9YI37)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     11     238       ATP-grasp        
end case
BINDING     55     57       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-R-G  
BINDING     330     332       /ligand="substrate" /ligand_note="ligand shared with subunit alpha     G-I-[VM]  
BINDING     208     208       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     N   1
BINDING     222     222       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D   1
BINDING     48     48       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     K  
BINDING     273     273       /ligand="substrate" /ligand_note="ligand shared with subunit alpha     N  
SITE     44     44       Important for substrate specificity     [DN]  
SITE     112     112       Important for substrate specificity     [YF]  

Additional information [?]

Size range 405-503 amino acids
Related rules MF_00558 (SUCC); MF_03221 (SUCB2)
Fusion None
Comments In animals, two different isoforms fo SCS exist, one specific for ATP and the other specific for GTP (PubMed:9765291). The two isoforms include the same alpha-subunit, but different beta-subunits (PubMed:9765290). This is the subfamily of ATP-specific beta subunits.