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Annotation rule MF_03221 |
General rule information
[?]
Accession |
MF_03221 |
Dates |
20-OCT-2016 (Created) 20-NOV-2019 (Last updated, Version 5) |
case <OC:Metazoa> or <OC:Amoebozoa>
end case
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial;
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EC 6.2.1.4;
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AltName:
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Full=GTP-specific succinyl-CoA synthetase subunit beta;
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Short=G-SCS;
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Short=GTPSCS;
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AltName:
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Full=Succinyl-CoA synthetase beta-G chain;
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Short=SCS-betaG;
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case <OC:Vertebrata>
end case
Function |
GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. |
Catalytic activity |
RHEA:22120: CoA + GTP + succinate = GDP + phosphate + succinyl-CoA
EC 6.2.1.4
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case <FTGroup:1>
Cofactor |
Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
end case
Pathway |
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. |
Subunit |
Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP. |
Subcellular location |
Mitochondrion. |
Similarity |
Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily. |
case not <AnyFeature:TransitM>
Miscellaneous |
This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case
case <FTGroup:1>
end case
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0004775; Molecular function: succinate-CoA ligase (ADP-forming) activity.
GO:0006099; Biological process: tricarboxylic acid cycle.
General |
TransitM; -; 0-1; trigger=yes; |
case <Feature:PS50975>
From: SUCB2_PIG (P53590) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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DOMAIN
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47
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275
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ATP-grasp
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end case
NP_BIND
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91
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93
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GTP
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G-R-G
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REGION
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366
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368
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Substrate binding; shared with subunit alpha
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G-I-[VM]
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METAL
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244
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244
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Magnesium
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N
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1
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METAL
|
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258
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258
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Magnesium
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D
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1
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BINDING
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58
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58
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GTP
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Q
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BINDING
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147
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147
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GTP; via amide nitrogen and carbonyl oxygen
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BINDING
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309
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309
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Substrate; shared with subunit alpha
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N
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SITE
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80
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80
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Important for substrate specificity
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E
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SITE
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148
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148
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Important for substrate specificity
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[DN]
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Additional information
[?]
Size range |
367-542 amino acids |
Related rules |
MF_00558 (SUCC); MF_03220 (SUCB1) |
Fusion |
None |
Comments |
In animals, two different isoforms of SCS exist, one specific for ATP and the other specific for GTP (PubMed:9765291). The two isoforms include the same alpha-subunit, but different beta-subunits (PubMed:9765290). This is the subfamily of GTP-specific beta subunits. |