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HAMAP rule MF_03221
General rule information
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| Accession | MF_03221 |
| Dates | 20-OCT-2016 (Created)
1-JUN-2023 (Last updated, Version 9) |
| Name | Succ_CoA_betaG_euk |
| Scope(s) |
Eukaryota |
| Template(s) | P53590 (SUCB2_PIG); [ Recover all ] |
| Triggered by |
case c? <OC:Metazoa> or <OC:Amoebozoa>
HAMAP; MF_03221 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SUCB2 |
| Protein name | RecName: Full=Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial; EC=6.2.1.4; AltName: Full=GTP-specific succinyl-CoA synthetase subunit beta; Short=G-SCS; Short=GTPSCS; AltName: Full=Succinyl-CoA synthetase beta-G chain; Short=SCS-betaG; |
| case <OC:Vertebrata> | |
| Gene name | Name=SUCLG2; |
| end case | |
Comments
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| FUNCTION | GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. |
| CATALYTIC ACTIVITY | Reaction=CoA + GTP + succinate = GDP + phosphate + succinyl-CoA; Xref=Rhea:RHEA:22120, ChEBI:CHEBI:30031, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:58189; EC=6.2.1.4; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| end case | |
| PATHWAY | Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. |
| SUBUNIT | Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP. |
| SUBCELLULAR LOCATION | Mitochondrion. |
| SIMILARITY | Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily. |
| case not <AnyFeature:TransitM> | |
| MISCELLANEOUS | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
| end case | |
Keywords
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| GTP-binding | |
| Ligase | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
| Mitochondrion | |
| Nucleotide-binding | |
| Tricarboxylic acid cycle | |
Gene Ontology
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| GO:0005525; Molecular function:GTP binding | |
| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| GO:0004775; Molecular function:succinate-CoA ligase (ADP-forming) activity | |
| GO:0006099; Biological process:tricarboxylic acid cycle | |
Cross-references
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| PROSITE | PS01217; SUCCINYL_COA_LIG_3; 1; |
| PROSITE | PS50975; ATP_GRASP; 1; |
| Pfam | PF08442; ATP-grasp_2; 1; |
| Pfam | PF00549; Ligase_CoA; 1; |
| NCBIfam | TIGR01016; sucCoAbeta; 1; |
| PIRSF | PIRSF001554; SucCS_beta; 1; |
| General | TransitM; -; 0-1; |
Features
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| From: SUCB2_PIG (P53590) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <Feature:PS50975> | ||||||||||||
| DOMAIN | 47 | 275 | /note="ATP-grasp" | |||||||||
| end case | ||||||||||||
| BINDING | 91 | 93 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-R-G | ||||||||
| BINDING | 366 | 368 | /ligand="substrate" /ligand_note="ligand shared with subunit alpha" |
G-I-[VM] | ||||||||
| BINDING | 244 | 244 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
N | 1 | |||||||
| BINDING | 258 | 258 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 58 | 58 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
Q | ||||||||
| BINDING | 147 | 147 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
|||||||||
| BINDING | 309 | 309 | /ligand="substrate" /ligand_note="ligand shared with subunit alpha" |
N | ||||||||
| SITE | 80 | 80 | /note="Important for substrate specificity" | E | ||||||||
| SITE | 148 | 148 | /note="Important for substrate specificity" | [DN] | ||||||||
Additional information
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| Size range | 367-542 amino acids |
| Related rules |
MF_00558 MF_03220 |
| Fusion | Nter: None Cter: None |
| Comments | In animals, two different isoforms of SCS exist, one specific for ATP and the other specific for GTP (PubMed:9765291). The two isoforms include the same alpha-subunit, but different beta-subunits (PubMed:9765290). This is the subfamily of GTP-specific beta subunits. |