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HAMAP rule MF_03221

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General rule information [?]

Accession MF_03221
Dates 20-OCT-2016 (Created)
1-JUN-2023 (Last updated, Version 9)
Name Succ_CoA_betaG_euk
Scope
Eukaryota
Template P53590 (SUCB2_PIG)
case <OC:Metazoa> or <OC:Amoebozoa>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SUCB2
Protein name
RecName: Full=Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial;
EC 6.2.1.4;
AltName: Full=GTP-specific succinyl-CoA synthetase subunit beta;
Short=G-SCS;
Short=GTPSCS;
AltName: Full=Succinyl-CoA synthetase beta-G chain;
Short=SCS-betaG;
case <OC:Vertebrata>
Gene name
SUCLG2
end case

Comments [?]

Function GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Catalytic activity RHEA:22120: CoA + GTP + succinate = GDP + phosphate + succinyl-CoA
EC 6.2.1.4
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
Pathway Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Subunit Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.
Subcellular location Mitochondrion.
Similarity Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.
case not <AnyFeature:TransitM>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case

Keywords [?]

GTP-binding
Ligase
case <FTGroup:1>
Magnesium
Metal-binding
end case
Mitochondrion
Nucleotide-binding
Tricarboxylic acid cycle

Gene Ontology [?]

GO:0005525; Molecular function: GTP binding.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0004775; Molecular function: succinate-CoA ligase (ADP-forming) activity.
GO:0006099; Biological process: tricarboxylic acid cycle.

Cross-references [?]

PROSITE PS01217; SUCCINYL_COA_LIG_3; 1;
PS50975; ATP_GRASP; 1;
Pfam PF08442; ATP-grasp_2; 1;
PF00549; Ligase_CoA; 1;
NCBIfam TIGR01016; sucCoAbeta; 1;
PIRSF PIRSF001554; SucCS_beta; 1;

Computed features [?]

General TransitM; -; 0-1; trigger=yes;

Features [?]

case <Feature:PS50975>
From: SUCB2_PIG (P53590)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     47     275       ATP-grasp        
end case
BINDING     91     93       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     G-R-G  
BINDING     366     368       /ligand="substrate" /ligand_note="ligand shared with subunit alpha     G-I-[VM]  
BINDING     244     244       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     N   1
BINDING     258     258       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D   1
BINDING     58     58       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     Q  
BINDING     147     147       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565        
BINDING     309     309       /ligand="substrate" /ligand_note="ligand shared with subunit alpha     N  
SITE     80     80       Important for substrate specificity     E  
SITE     148     148       Important for substrate specificity     [DN]  

Additional information [?]

Size range 367-542 amino acids
Related rules MF_00558 (SUCC); MF_03220 (SUCB1)
Fusion None
Comments In animals, two different isoforms of SCS exist, one specific for ATP and the other specific for GTP (PubMed:9765291). The two isoforms include the same alpha-subunit, but different beta-subunits (PubMed:9765290). This is the subfamily of GTP-specific beta subunits.


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