HAMAP rule MF_03221
General rule information
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Accession | MF_03221 |
Dates | 20-OCT-2016 (Created) 1-JUN-2023 (Last updated, Version 9) |
Name | Succ_CoA_betaG_euk |
Scope | Eukaryota |
Template | P53590 (SUCB2_PIG) |
case <OC:Metazoa> or <OC:Amoebozoa>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
Protein name |
|
case <OC:Vertebrata>
Gene name |
|
end case
Comments
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Function | GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. |
Catalytic activity | RHEA:22120: CoA + GTP + succinate = GDP + phosphate + succinyl-CoA
EC 6.2.1.4 |
case <FTGroup:1>
Cofactor | Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
end case
Pathway | Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. |
Subunit | Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP. |
Subcellular location | Mitochondrion. |
Similarity | Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily. |
case not <AnyFeature:TransitM>
Miscellaneous | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case
Keywords
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case <FTGroup:1>
end case
Gene Ontology
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GO:0005525; Molecular function: GTP binding.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0004775; Molecular function: succinate-CoA ligase (ADP-forming) activity.
GO:0006099; Biological process: tricarboxylic acid cycle.
GO:0006099; Biological process: tricarboxylic acid cycle.
Cross-references
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PROSITE | PS01217; SUCCINYL_COA_LIG_3; 1; |
PS50975; ATP_GRASP; 1; | |
Pfam | PF08442; ATP-grasp_2; 1; |
PF00549; Ligase_CoA; 1; | |
NCBIfam | TIGR01016; sucCoAbeta; 1; |
PIRSF | PIRSF001554; SucCS_beta; 1; |
Computed features
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General | TransitM; -; 0-1; trigger=yes; |
Features
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case <Feature:PS50975>
From: SUCB2_PIG (P53590) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
DOMAIN | 47 | 275 | ATP-grasp |
end case
Additional information
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Size range | 367-542 amino acids |
Related rules | MF_00558 (SUCC); MF_03220 (SUCB1) |
Fusion | None |
Comments | In animals, two different isoforms of SCS exist, one specific for ATP and the other specific for GTP (PubMed:9765291). The two isoforms include the same alpha-subunit, but different beta-subunits (PubMed:9765290). This is the subfamily of GTP-specific beta subunits. |