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HAMAP rule MF_04000

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General rule information [?]

Accession MF_04000
Dates 29-OCT-2015 (Created)
19-NOV-2022 (Last updated, Version 8)
Name PPV_E1
Scope(s) Viruses
Template(s) P03116 (VE1_BPV1); P04014 (VE1_HPV11); [ Recover all ]
Triggered by HAMAP; MF_04000 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier VE1
RecName: Full=Replication protein E1;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/"></a>;<br /> AltName: Full=ATP-dependent helicase E1;
Gene name Name=E1;

Comments [?]

FUNCTIONATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1- E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.
CATALYTIC ACTIVITY Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=;
SUBUNITCan form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA- binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.
case <FTTag:Ubl>
end case
SIMILARITYBelongs to the papillomaviridae E1 protein family.

Keywords [?]

DNA replication
Early protein
<a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a>
Host nucleus
case <FTTag:Ubl>
Ubl conjugation
Isopeptide bond
end case

Gene Ontology [?]

GO:0042025; Cellular component:host cell nucleus
GO:0005524; Molecular function:ATP binding
GO:0003678; Molecular function:DNA helicase activity
GO:0003677; Molecular function:DNA binding
GO:0006260; Biological process:DNA replication

Cross-references [?]

Pfam PF00519; PPV_E1_C; 1;
Pfam PF00524; PPV_E1_N; 1;
PIRSF PIRSF003383; Rep_E1_papillomaV; 1;

Features [?]

From: VE1_BPV1 (P03116)
Key From To Description Tag Condition FTGroup
BINDING 433 440 /ligand="ATP"
MOTIF 84 86 /note="Nuclear localization signal" K-R-K
REGION 142 308 /note="DNA-binding region"
MOD_RES 102 102 /note="Phosphothreonine; by host" T
MOD_RES 109 109 /note="Phosphoserine; by host" S
CROSSLNK 514 514 /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)" Ubl K
From: VE1_HPV11 (P04014)
Key From To Description Tag Condition FTGroup
MOTIF 106 115 /note="Nuclear export signal" [LIV]-x-x-x-[LIV]-x-x-[LIV]-x-[LIV]
MOD_RES 89 89 /note="Phosphoserine; by host" S
MOD_RES 93 93 /note="Phosphoserine; by host" S
MOD_RES 107 107 /note="Phosphoserine; by host" S

Additional information [?]

Size range 580-700 amino acids
Related rules None
Fusion Nter: None Cter: None

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