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HAMAP rule MF_04000

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General rule information [?]

Accession	MF_04000
Dates	29-OCT-2015 (Created) 19-NOV-2022 (Last updated, Version 8)
Name	PPV_E1
Scope(s)	Viruses Papillomaviridae
Template(s)	P03116 (VE1_BPV1); P04014 (VE1_HPV11); [ Recover all ]
Triggered by	HAMAP; MF_04000 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	VE1
RecName: Full=Replication protein E1;<br />                  EC=<a href="https://enzyme.expasy.org/EC/3.6.4.12">3.6.4.12</a>;<br /> AltName: Full=ATP-dependent helicase E1;
Gene name	Name=E1;

Comments [?]

FUNCTION	ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1- E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.
CATALYTIC ACTIVITY	Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
SUBUNIT	Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA- binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.
SUBCELLULAR LOCATION	Host nucleus.
PTM	Phosphorylated.
case <FTTag:Ubl>
PTM	Sumoylated.
end case
SIMILARITY	Belongs to the papillomaviridae E1 protein family.

Keywords [?]

ATP-binding
DNA replication
DNA-binding
Early protein
<a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a>
Host nucleus
Hydrolase
Nucleotide-binding
Phosphoprotein
case <FTTag:Ubl>
Ubl conjugation
Isopeptide bond
end case

Gene Ontology [?]

GO:0042025; Cellular component:host cell nucleus

GO:0005524; Molecular function:ATP binding

GO:0003678; Molecular function:DNA helicase activity

GO:0003677; Molecular function:DNA binding

GO:0006260; Biological process:DNA replication

Cross-references [?]

PROSITE	PS51206; SF3_HELICASE_1; 1;
Pfam	PF00519; PPV_E1_C; 1;
Pfam	PF00524; PPV_E1_N; 1;
PIRSF	PIRSF003383; Rep_E1_papillomaV; 1;

Features [?]

From: VE1_BPV1 (P03116)

Key

From

Description

Tag

Condition

FTGroup

BINDING

433

440

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

G-x-x-x-x-G-K-S

MOTIF

/note="Nuclear localization signal"

K-R-K

REGION

142

308

/note="DNA-binding region"

MOD_RES

102

/note="Phosphothreonine; by host"

MOD_RES

109

/note="Phosphoserine; by host"

CROSSLNK

514

/note="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)"

Ubl

From: VE1_HPV11 (P04014)

Key

From

Description

Tag

Condition

FTGroup

MOTIF

106

115

/note="Nuclear export signal"

[LIV]-x-x-x-[LIV]-x-x-[LIV]-x-[LIV]

MOD_RES

/note="Phosphoserine; by host"

MOD_RES

/note="Phosphoserine; by host"

MOD_RES

107

/note="Phosphoserine; by host"

Additional information [?]

Size range	580-700 amino acids
Related rules	None
Fusion	Nter: None Cter: None

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