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HAMAP rule MF_04000

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General rule information [?]

Accession MF_04000
Dates 29-OCT-2015 (Created)
19-NOV-2022 (Last updated, Version 8)
Name PPV_E1
Viruses; Papillomaviridae
Templates P03116 (VE1_BPV1); P04014 (VE1_HPV11): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Replication protein E1;
AltName: Full=ATP-dependent helicase E1;
Gene name

Comments [?]

Function ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.
Catalytic activity RHEA:13065: ATP + H2O = ADP + H(+) + phosphate
Subunit Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.
Subcellular location Host nucleus.
Ptm Phosphorylated.
case <FTTag:Ubl>
Ptm Sumoylated.
end case
Similarity Belongs to the papillomaviridae E1 protein family.

Keywords [?]

case <FTTag:Ubl>
end case

Gene Ontology [?]

GO:0042025; Cellular component: host cell nucleus.
GO:0005524; Molecular function: ATP binding.
GO:0003678; Molecular function: DNA helicase activity.
GO:0003677; Molecular function: DNA binding.
GO:0006260; Biological process: DNA replication.

Cross-references [?]

PROSITE PS51206; SF3_HELICASE_1; 1; trigger=PRU00551;
Pfam PF00519; PPV_E1_C; 1;
PF00524; PPV_E1_N; 1;
PIRSF PIRSF003383; Rep_E1_papillomaV; 1;

Features [?]

From: VE1_BPV1 (P03116)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     433     440       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-x-x-x-x-G-K-S  
MOTIF     84     86       Nuclear localization signal     K-R-K  
REGION     142     308       DNA-binding region        
MOD_RES     102     102       Phosphothreonine; by host     T  
MOD_RES     109     109       Phosphoserine; by host     S  
CROSSLNK     514     514       Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)   Ubl   K  
From: VE1_HPV11 (P04014)
MOTIF (Optional)     106     115       Nuclear export signal     [LIV]-x-x-x-[LIV]-x-x-[LIV]-x-[LIV]  
MOD_RES (Optional)     89     89       Phosphoserine; by host     S  
MOD_RES (Optional)     93     93       Phosphoserine; by host     S  
MOD_RES (Optional)     107     107       Phosphoserine; by host     S  

Additional information [?]

Size range 580-700 amino acids
Related rules None
Fusion None