HAMAP rule MF_04000
General rule information
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| Accession | MF_04000 |
| Dates | 29-OCT-2015 (Created)
19-NOV-2022 (Last updated, Version 8) |
| Name | PPV_E1 |
| Scope(s) |
Viruses Papillomaviridae |
| Template(s) | P03116 (VE1_BPV1); P04014 (VE1_HPV11); [ Recover all ] |
| Triggered by |
HAMAP; MF_04000 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | VE1 |
| RecName: Full=Replication protein E1;<br /> EC=<a href="https://enzyme.expasy.org/EC/3.6.4.12">3.6.4.12</a>;<br /> AltName: Full=ATP-dependent helicase E1; | |
| Gene name | Name=E1; |
Comments
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| FUNCTION | ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1- E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication. |
| CATALYTIC ACTIVITY | Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; |
| SUBUNIT | Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA- binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1. |
| SUBCELLULAR LOCATION | Host nucleus. |
| PTM | Phosphorylated. |
| case <FTTag:Ubl> | |
| PTM | Sumoylated. |
| end case | |
| SIMILARITY | Belongs to the papillomaviridae E1 protein family. |
Keywords
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| ATP-binding | |
| DNA replication | |
| DNA-binding | |
| Early protein | |
| <a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a> | |
| Host nucleus | |
| Hydrolase | |
| Nucleotide-binding | |
| Phosphoprotein | |
| case <FTTag:Ubl> | |
| Ubl conjugation | |
| Isopeptide bond | |
| end case | |
Gene Ontology
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| GO:0042025; Cellular component:host cell nucleus |
| GO:0005524; Molecular function:ATP binding |
| GO:0003678; Molecular function:DNA helicase activity |
| GO:0003677; Molecular function:DNA binding |
| GO:0006260; Biological process:DNA replication |
Cross-references
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| PROSITE | PS51206; SF3_HELICASE_1; 1; |
| Pfam | PF00519; PPV_E1_C; 1; |
| Pfam | PF00524; PPV_E1_N; 1; |
| PIRSF | PIRSF003383; Rep_E1_papillomaV; 1; |
Features
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| From: VE1_BPV1 (P03116) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 433 | 440 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-x-x-x-x-G-K-S | ||||||||
| MOTIF | 84 | 86 | /note="Nuclear localization signal" | K-R-K | ||||||||
| REGION | 142 | 308 | /note="DNA-binding region" | |||||||||
| MOD_RES | 102 | 102 | /note="Phosphothreonine; by host" | T | ||||||||
| MOD_RES | 109 | 109 | /note="Phosphoserine; by host" | S | ||||||||
| CROSSLNK | 514 | 514 | /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)" | Ubl | K | |||||||
| From: VE1_HPV11 (P04014) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 106 | 115 | /note="Nuclear export signal" | [LIV]-x-x-x-[LIV]-x-x-[LIV]-x-[LIV] | ||||||||
| MOD_RES | 89 | 89 | /note="Phosphoserine; by host" | S | ||||||||
| MOD_RES | 93 | 93 | /note="Phosphoserine; by host" | S | ||||||||
| MOD_RES | 107 | 107 | /note="Phosphoserine; by host" | S | ||||||||
Additional information
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| Size range | 580-700 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |