HAMAP rule MF_04008

General rule information [?]

Accession	MF_04008
Dates	11-FEB-2016 (Created) 26-JUL-2022 (Last updated, Version 7)

Name

HSV_SCAF

Scope

Viruses; Herpesvirales

Template

P10210 (SCAF_HHV11)

Triggered by

HAMAP; MF_04008 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

SCAF

Protein name

RecName:		Full=Capsid scaffolding protein;
AltName:		Full=Protease precursor;
		Short=pPR;
Contains:
	RecName:		Full=Assemblin;
			EC 3.4.21.97;
	AltName:		Full=Protease;
Contains:
	RecName:		Full=Assembly protein;
	AltName:		Full=Capsid assembly protein;

Comments [?]

Function	Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging.
	Assemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.
	Assembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.
Catalytic activity	Reaction=Cleaves -Ala-\|-Ser- and -Ala-\|-Ala- bonds in the scaffold protein.; EC=3.4.21.97;
Subunit	[Capsid scaffolding protein]: Homomultimer. Interacts with major capsid protein.
	[Assemblin]: Exists in a monomer-dimer equilibrium with the dimer being the active species.
	[Assembly protein]: Homomultimer. Interacts with major capsid protein.
Subcellular location	Capsid scaffolding protein: Host cytoplasm.
	Assemblin: Host nucleus.
	Assembly protein: Host nucleus.
Domain	Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD).
Ptm	Capsid scaffolding protein is cleaved by assemblin after formation of the spherical procapsid. As a result, the capsid obtains its mature, icosahedral shape. Cleavages occur at two or more sites: release and tail site.
Similarity	Belongs to the herpesviridae capsid scaffolding protein family.

Keywords [?]

Host cytoplasm
Host nucleus
Hydrolase
Phosphoprotein
Protease
Serine protease
Viral capsid assembly
Viral release from host cell

Gene Ontology [?]

GO:0030430; Cellular component: host cell cytoplasm.
GO:0042025; Cellular component: host cell nucleus.
GO:0042802; Molecular function: identical protein binding.
GO:0004252; Molecular function: serine-type endopeptidase activity.
GO:0019076; Biological process: viral release from host cell.

Cross-references [?]

Pfam	PF00716; Peptidase_S21; 1;
PRINTS	PR00236; HSVCAPSIDP40; 1;

Features [?]

From: SCAF_HHV11 (P10210)

Key From To Description Tag Condition FTGroup

CHAIN Nter Cter Capsid scaffolding protein

CHAIN Nter 247 Assemblin

CHAIN 248 Cter Assembly protein

REGION 324 343 Interaction with pAP

REGION Cter-20 Cter Interaction with major capsid protein

ACT_SITE 61 61 Charge relay system H

ACT_SITE 129 129 Charge relay system S

ACT_SITE 148 148 Charge relay system H

SITE 247 248 Cleavage; by assemblin; Release site A-x

SITE 610 611 Cleavage; by assemblin; Tail site A-S

Additional information [?]

Size range	475-726 amino acids
Related rules	None
Fusion	None

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