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HAMAP rule MF_04008

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General rule information [?]

Accession MF_04008
Dates 11-FEB-2016 (Created)
21-MAR-2024 (Last updated, Version 8)
Name HSV_SCAF
Scope(s) Viruses
Herpesvirales
Template(s) P10210 (SCAF_HHV11); [ Recover all ]
Triggered by HAMAP; MF_04008 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SCAF
Protein name RecName: Full=Capsid scaffolding protein;
AltName: Full=Protease precursor;
                 Short=pPR;
                 Contains:
RecName: Full=Assemblin;
                 EC=3.4.21.97;
AltName: Full=Protease;
                 Short=Pr;
                 Contains:
RecName: Full=Assembly protein;
                 Short=AP;
AltName: Full=Capsid assembly protein;

Comments [?]

FUNCTIONCapsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging.
FUNCTIONAssemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.
FUNCTIONAssembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.
CATALYTIC ACTIVITY Reaction=Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.; EC=3.4.21.97;
SUBUNIT[Capsid scaffolding protein]: Homomultimer. Interacts with major capsid protein.
SUBUNIT[Assemblin]: Exists in a monomer-dimer equilibrium with the dimer being the active species.
SUBUNIT[Assembly protein]: Homomultimer. Interacts with major capsid protein.
SUBCELLULAR LOCATIONCapsid scaffolding protein: Host cytoplasm.
SUBCELLULAR LOCATIONAssemblin: Host nucleus.
SUBCELLULAR LOCATIONAssembly protein: Host nucleus.
DOMAINRegion of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD).
PTMCapsid scaffolding protein: Capsid scaffolding protein is cleaved by assemblin after formation of the spherical procapsid. As a result, the capsid obtains its mature, icosahedral shape. Cleavages occur at two or more sites: release (R-site) and maturation (M-site).
SIMILARITYBelongs to the herpesviridae capsid scaffolding protein family.

Keywords [?]


Gene Ontology [?]

GO:0030430; Cellular component:host cell cytoplasm
GO:0042025; Cellular component:host cell nucleus
GO:0042802; Molecular function:identical protein binding
GO:0004252; Molecular function:serine-type endopeptidase activity
GO:0019076; Biological process:viral release from host cell

Cross-references [?]

Pfam PF00716; Peptidase_S21; 1;
PRINTS PR00236; HSVCAPSIDP40; 1;

Features [?]

From: SCAF_HHV11 (P10210)
Key From To Description Tag Condition FTGroup
CHAIN Nter Cter /note="Capsid scaffolding protein"
CHAIN Nter 247 /note="Assemblin"
CHAIN 248 Cter /note="Assembly protein"
REGION 324 343 /note="Interaction with pAP"
REGION Cter-20 Cter /note="Interaction with major capsid protein"
ACT_SITE 61 61 /note="Charge relay system" H
ACT_SITE 129 129 /note="Charge relay system" S
ACT_SITE 148 148 /note="Charge relay system" H
SITE 247 248 /note="Cleavage; by assemblin; Release site" A-x
SITE 610 611 /note="Cleavage; by assemblin; Tail site" A-S

Additional information [?]

Size range 475-726 amino acids
Related rules None
Fusion Nter: None Cter: None



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