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| Annotation rule MF_04008 |
General rule information
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| Accession | MF_04008 |
| Dates | 11-FEB-2016 (Created) 20-NOV-2019 (Last updated, Version 6) |
| Name | HSV_SCAF |
| Scope | Viruses; Herpesvirales |
| Template | P10210 (SCAF_HHV11) |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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Comments
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| Function | Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging. |
| Assemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging. | |
| Assembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging. | |
| Catalytic activity | Reaction=Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.; EC=3.4.21.97; |
| Subunit | Capsid scaffolding protein homomultimerizes and interacts with major capsid protein. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein. |
| Subcellular location | Capsid scaffolding protein: Host cytoplasm. |
| Assemblin: Host nucleus. | |
| Assembly protein: Host nucleus. | |
| Domain | Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD). |
| Ptm | Capsid scaffolding protein is cleaved by assemblin after formation of the spherical procapsid. As a result, the capsid obtains its mature, icosahedral shape. Cleavages occur at two or more sites: release and tail site. |
| Similarity | Belongs to the herpesviridae capsid scaffolding protein family. |
Keywords
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Host cytoplasm
Host nucleus
Hydrolase
Phosphoprotein
Protease
Serine protease
Viral capsid assembly
Viral release from host cell
Host nucleus
Hydrolase
Phosphoprotein
Protease
Serine protease
Viral capsid assembly
Viral release from host cell
Gene Ontology
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GO:0030430; Cellular component: host cell cytoplasm.
GO:0042025; Cellular component: host cell nucleus.
GO:0042802; Molecular function: identical protein binding.
GO:0004252; Molecular function: serine-type endopeptidase activity.
GO:0019076; Biological process: viral release from host cell.
GO:0042025; Cellular component: host cell nucleus.
GO:0042802; Molecular function: identical protein binding.
GO:0004252; Molecular function: serine-type endopeptidase activity.
GO:0019076; Biological process: viral release from host cell.
Cross-references
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Features
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| From: SCAF_HHV11 (P10210) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| CHAIN | Nter | Cter | Capsid scaffolding protein | |||||||||
| CHAIN | Nter | 247 | Assemblin | |||||||||
| CHAIN | 248 | Cter | Assembly protein | |||||||||
| REGION | 324 | 343 | Interaction with pAP | |||||||||
| REGION | Cter-20 | Cter | Interaction with major capsid protein | |||||||||
| ACT_SITE | 61 | 61 | Charge relay system | H | ||||||||
| ACT_SITE | 129 | 129 | Charge relay system | S | ||||||||
| ACT_SITE | 148 | 148 | Charge relay system | H | ||||||||
| SITE | 247 | 248 | Cleavage; by assemblin; Release site | A-x | ||||||||
| SITE | 610 | 611 | Cleavage; by assemblin; Tail site | A-S | ||||||||
Additional information
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| Size range | 475-726 amino acids |
| Related rules | None |
| Fusion | None |