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Annotation rule MF_04008
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General rule information [?]

Accession MF_04008
Dates 11-FEB-2016 (Created)
20-NOV-2019 (Last updated, Version 6)
Name HSV_SCAF
Scope
Viruses; Herpesvirales
Template P10210 (SCAF_HHV11)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SCAF
Protein name
RecName: Full=Capsid scaffolding protein;
AltName: Full=Protease precursor;
Short=pPR;
Contains:
RecName: Full=Assemblin;
EC 3.4.21.97;
AltName: Full=Protease;
Contains:
RecName: Full=Assembly protein;
AltName: Full=Capsid assembly protein;

Comments [?]

Function Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging.
Assemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.
Assembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.
Catalytic activity Reaction=Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.; EC=3.4.21.97;
Subunit Capsid scaffolding protein homomultimerizes and interacts with major capsid protein. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein.
Subcellular location Capsid scaffolding protein: Host cytoplasm.
Assemblin: Host nucleus.
Assembly protein: Host nucleus.
Domain Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD).
Ptm Capsid scaffolding protein is cleaved by assemblin after formation of the spherical procapsid. As a result, the capsid obtains its mature, icosahedral shape. Cleavages occur at two or more sites: release and tail site.
Similarity Belongs to the herpesviridae capsid scaffolding protein family.

Keywords [?]


Gene Ontology [?]

GO:0030430; Cellular component: host cell cytoplasm.
GO:0042025; Cellular component: host cell nucleus.
GO:0042802; Molecular function: identical protein binding.
GO:0004252; Molecular function: serine-type endopeptidase activity.
GO:0019076; Biological process: viral release from host cell.

Cross-references [?]

Pfam PF00716; Peptidase_S21; 1;
PRINTS PR00236; HSVCAPSIDP40; 1;

Features [?]

From: SCAF_HHV11 (P10210)
Key     From     To       Description   Tag   Condition   FTGroup
CHAIN     Nter     Cter       Capsid scaffolding protein        
CHAIN     Nter     247       Assemblin        
CHAIN     248     Cter       Assembly protein        
REGION     324     343       Interaction with pAP        
REGION     Cter-20     Cter       Interaction with major capsid protein        
ACT_SITE     61     61       Charge relay system     H  
ACT_SITE     129     129       Charge relay system     S  
ACT_SITE     148     148       Charge relay system     H  
SITE     247     248       Cleavage; by assemblin; Release site     A-x  
SITE     610     611       Cleavage; by assemblin; Tail site     A-S  

Additional information [?]

Size range 475-726 amino acids
Related rules None
Fusion None