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HAMAP rule MF_04009

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General rule information [?]

Accession MF_04009
Dates 16-FEB-2016 (Created)
1-MAR-2024 (Last updated, Version 7)
Name HSV_AN
Scope(s) Viruses
Herpesviridae
Template(s) P04294 (AN_HHV11); Q2HR95 (AN_HHV8P); P03217 (AN_EBVB9); [ Recover all ]
Triggered by HAMAP; MF_04009 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier AN
case <OC:Gammaherpesvirinae>
Protein name RecName: Full=Shutoff alkaline exonuclease;
                 Short=SOX;
                 EC=3.1.-.-;
else
Protein name RecName: Full=Alkaline nuclease;
                 EC=3.1.-.-;
end case

Comments [?]

case <OC:Gammaherpesvirinae>
FUNCTIONPlays a role in processing non linear or branched viral DNA intermediates in order to promote the production of mature packaged unit-length linear progeny viral DNA molecules. Exhibits endonuclease and exonuclease activities and accepts both double-stranded and single- stranded DNA as substrate. Exonuclease digestion of DNA is in the 5'-> 3' direction and the products are 5'-monophosphate nucleosides. Additionally, forms a recombinase with the major DNA-binding protein, which displays strand exchange activity. Also acts as a cytoplasmic RNA endonuclease that induces degradation of the majority of the cellular messenger RNAs during early lytic infection. The resulting inhibition of cellular protein synthesis serves to ensure maximal viral gene expression and evasion from host immune response. Internally cleaves host mRNAs which are then degraded by the cellular exonuclease XRN1. Bypasses therefore the regulatory steps of deadenylation and decapping normally required for XRN1 activation.
else
FUNCTIONPlays a role in processing non linear or branched viral DNA intermediates in order to promote the production of mature packaged unit-length linear progeny viral DNA molecules. Exhibits endonuclease and exonuclease activities and accepts both double-stranded and single- stranded DNA as substrate. Exonuclease digestion of DNA is in the 5'-> 3' direction and the products are 5'-monophosphate nucleosides. Additionally, forms a recombinase with the major DNA-binding protein, which displays strand exchange activity.
end case
case <OC:Gammaherpesvirinae>
SUBUNITForms a complex with the DNA polymerase, the DNA polymerase processivity factor, and the major DNA binding protein.
else
SUBUNITInteracts with major DNA-binding protein; this interaction increases the nuclease processivity of the alkaline exonuclease.
end case
SUBCELLULAR LOCATIONHost nucleus. Host cytoplasm.
SIMILARITYBelongs to the herpesviridae alkaline nuclease family.

Keywords [?]

Endonuclease
Exonuclease
Host cytoplasm
Host nucleus
Host-virus interaction
Hydrolase
Nuclease

Gene Ontology [?]

case <OCellular component:Gammaherpesvirinae>
GO:0030430; Cellular component:host cell cytoplasm
GO:0042025; Cellular component:host cell nucleus
GO:0003677; Molecular function:DNA binding
GO:0004519; Molecular function:endonuclease activity
GO:0004527; Molecular function:exonuclease activity
GO:0004518; Molecular function:nuclease activity
GO:0090304; Biological process:nucleic acid metabolic process
GO:0016032; Biological process:viral process
GO:0003723; Molecular function:RNA binding
GO:0039595; Biological process:symbiont-mediated degradation of host mRNA
GO:0039657; Biological process:symbiont-mediated suppression of host gene expression
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0030430; Cellular component:host cell cytoplasm
GO:0042025; Cellular component:host cell nucleus
GO:0003677; Molecular function:DNA binding
GO:0004519; Molecular function:endonuclease activity
GO:0004527; Molecular function:exonuclease activity
GO:0004518; Molecular function:nuclease activity
GO:0090304; Biological process:nucleic acid metabolic process
GO:0016032; Biological process:viral process
end case

Cross-references [?]

Pfam PF01771; Herpes_alk_exo; 1;
PRINTS PR00924; ALKEXNUCLASE; 1;

Features [?]

From: AN_HHV11 (P04294)
Key From To Description Tag Condition FTGroup
SITE 280 280 /note="Required for function" E
SITE 340 340 /note="Required for function" D
SITE 364 364 /note="Required for function" E
SITE 366 366 /note="Required for function" K

Additional information [?]

Size range 470-626 amino acids
Related rules None
Fusion Nter: None Cter: None



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