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HAMAP rule MF_04059

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General rule information [?]

Accession MF_04059
Dates 16-MAR-2017 (Created)
4-JUN-2021 (Last updated, Version 7)
Viruses; Adenoviridae
Template P03252 (PRO_ADE02)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Protease;
AltName: Full=Adenain;
AltName: Full=Adenovirus protease;
AltName: Full=Adenovirus proteinase;
AltName: Full=Endoprotease;
Gene name

Comments [?]

Function Cleaves viral precursor proteins (pTP, pIIIa, pVI, pVII, pVIII, and pX) inside newly assembled particles giving rise to mature virions. Protease complexed to its cofactor slides along the viral DNA to specifically locate and cleave the viral precursors. Mature virions have a weakened organization compared to the unmature virions, thereby facilitating subsequent uncoating. Without maturation, the particle lacks infectivity and is unable to uncoat. Late in adenovirus infection, in the cytoplasm, may participate in the cytoskeleton destruction. Cleaves host cell cytoskeletal keratins K7 and K18.
Catalytic activity Reaction=Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).; EC=;
Activity regulation Requires DNA and protease cofactor for maximal activation. Inside nascent virions, becomes partially activated by binding to the viral DNA, allowing it to cleave the cofactor that binds to the protease and fully activates it. Actin, like the viral protease cofactor, seems to act as a cofactor in the cleavage of cytokeratin 18 and of actin itself.
Subunit Interacts with protease cofactor pVI-C; this interaction is necessary for protease activation.
Subcellular location Virion. Host nucleus. Note=Present in about 10 copies per virion.
Induction Expressed in the late phase of the viral replicative cycle.
Miscellaneous All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.
Similarity Belongs to the peptidase C5 family.

Keywords [?]

Gene Ontology [?]

GO:0042025; Cellular component: host cell nucleus.
GO:0044423; Cellular component: virion component.
GO:0004197; Molecular function: cysteine-type endopeptidase activity.
GO:0008234; Molecular function: cysteine-type peptidase activity.
GO:0003677; Molecular function: DNA binding.

Cross-references [?]

Pfam PF00770; Peptidase_C5; 1;
PIRSF PIRSF001218; Protease_ADV; 1;

Features [?]

From: PRO_ADE02 (P03252)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     54     54             H  
ACT_SITE     71     71             [DE]  
ACT_SITE     122     122             C  
SITE     51     52       Cleavage; by autolysis     G-x  
DISULFID     104     104       Interchain (with C-10 in cleaved protease cofactor pVI-C)     C  

Additional information [?]

Size range 196-214 amino acids
Related rules None
Fusion None