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HAMAP rule MF_04060

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General rule information [?]

Accession MF_04060
Dates 16-MAR-2017 (Created)
20-NOV-2019 (Last updated, Version 3)
Scope(s) Viruses
Template(s) P24932 (SHUT_ADE02); [ Recover all ]
Triggered by HAMAP; MF_04060 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SHUT
Protein name RecName: Full=Shutoff protein;
AltName: Full=100 kDa protein;
AltName: Full=100K-chaperone protein;
AltName: Full=L4-100K;
AltName: Full=Shutoff protein 100K;
Gene name Name=L4;

Comments [?]

FUNCTIONProtein that inhibits host translation while promoting late viral translation by ribosome shunting. Blocks host cap-dependent translation by binding to eIF4G, displacing MKNK1 from cap initiation complexes and preventing EIF4E phosphorylation. Binds to the tripartite leader sequence of viral late mRNAs and recruits host eIF4G, PABPC1/poly-A binding protein and 40S ribosomes subunits on viral mRNAs, allowing ribosome shunting and efficient translation of late viral mRNAs even though conventional translation via ribosome scanning from the cap has been shut off in the host cell. During assembly, acts as a chaperone protein that helps hexon proteins assembly into trimers.
SUBUNITMonomer. Interacts with hexon protein; this interaction allows chaperoning and trimerization of hexon proteins. Interacts (via N- terminus) with host initiation factor EIF4G (via C-terminus). Interacts (via RRM domain) with viral mRNAs that contain the tripartite leader; this interaction allows ribosome shunting and expression of viral late mRNAs.
INDUCTIONExpressed in the late phase of the viral replicative cycle.
PTMPhosphorylated. Tyrosine phosphorylation enhances preferential binding to tripartite leader mRNAs and allows ribosome shunting.
PTMMethylated. Asymmetric dimethylation by host PRMT1 of the Arg/Gly- rich region may regulate shutoff protein binding to hexon and promote the capsid assembly in the nucleus.
PTMMight be cleaved by the viral protease.
MISCELLANEOUSAll late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.
SIMILARITYBelongs to the adenoviridae shutoff protein family.

Keywords [?]

Gene Ontology [?]

GO:0030430; Cellular component:host cell cytoplasm
GO:0003723; Molecular function:RNA binding
GO:0019060; Biological process:intracellular transport of viral protein in host cell
GO:0039657; Biological process:suppression by virus of host gene expression
GO:0039704; Biological process:viral translational shunt

Cross-references [?]

Pfam PF02438; Adeno_100; 1;

Features [?]

From: SHUT_ADE02 (P24932)
Key From To Description Tag Condition FTGroup
DOMAIN 348 466 /note="RRM"
REGION 280 345 /note="Binding to host EIF4G"
MOD_RES 365 365 /note="Phosphotyrosine; by host" Y
MOD_RES 682 682 /note="Phosphotyrosine; by host" Y

Additional information [?]

Size range 679-984 amino acids
Related rules None
Fusion Nter: None Cter: None

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