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Annotation rule MF_04060
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General rule information [?]

Accession MF_04060
Dates 16-MAR-2017 (Created)
20-NOV-2019 (Last updated, Version 3)
Name ADV_SHUT
Scope
Viruses; Adenoviridae
Template P24932 (SHUT_ADE02)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SHUT
Protein name
RecName: Full=Shutoff protein;
AltName: Full=100 kDa protein;
Short=p100K;
AltName: Full=100K-chaperone protein;
AltName: Full=L4-100K;
AltName: Full=Shutoff protein 100K;
Gene name
L4

Comments [?]

Function Protein that inhibits host translation while promoting late viral translation by ribosome shunting. Blocks host cap-dependent translation by binding to eIF4G, displacing MKNK1 from cap initiation complexes and preventing EIF4E phosphorylation. Binds to the tripartite leader sequence of viral late mRNAs and recruits host eIF4G, PABPC1/poly-A binding protein and 40S ribosomes subunits on viral mRNAs, allowing ribosome shunting and efficient translation of late viral mRNAs even though conventional translation via ribosome scanning from the cap has been shut off in the host cell. During assembly, acts as a chaperone protein that helps hexon proteins assembly into trimers.
Subunit Monomer. Interacts with hexon protein; this interaction allows chaperoning and trimerization of hexon proteins. Interacts (via N-terminus) with host initiation factor EIF4G (via C-terminus). Interacts (via RRM domain) with viral mRNAs that contain the tripartite leader; this interaction allows ribosome shunting and expression of viral late mRNAs.
Subcellular location Host cytoplasm.
Induction Expressed in the late phase of the viral replicative cycle.
Ptm Phosphorylated. Tyrosine phosphorylation enhances preferential binding to tripartite leader mRNAs and allows ribosome shunting.
Methylated. Asymmetric dimethylation by host PRMT1 of the Arg/Gly-rich region may regulate shutoff protein binding to hexon and promote the capsid assembly in the nucleus.
Might be cleaved by the viral protease.
Miscellaneous All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.
Similarity Belongs to the adenoviridae shutoff protein family.

Keywords [?]

Chaperone
Eukaryotic host gene expression shutoff by virus
Eukaryotic host translation shutoff by virus
Host cytoplasm
Host gene expression shutoff by virus
Host-virus interaction
Inhibition of eukaryotic host translation factors by virus
Late protein
Methylation
Phosphoprotein
RNA-binding
Translational shunt
Transport

Gene Ontology [?]

GO:0030430; Cellular component: host cell cytoplasm.
GO:0003723; Molecular function: RNA binding.
GO:0019060; Biological process: intracellular transport of viral protein in host cell.
GO:0039657; Biological process: suppression by virus of host gene expression.
GO:0039704; Biological process: viral translational shunt.

Cross-references [?]

Pfam PF02438; Adeno_100; 1;

Features [?]

From: SHUT_ADE02 (P24932)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     348     466       RRM        
REGION     280     345       Binding to host EIF4G        
MOD_RES     365     365       Phosphotyrosine; by host     Y  
MOD_RES     682     682       Phosphotyrosine; by host     Y  

Additional information [?]

Size range 679-984 amino acids
Related rules None
Fusion None


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