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Annotation rule MF_04084
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General rule information [?]

Accession MF_04084
Dates 12-JAN-2018 (Created)
20-NOV-2019 (Last updated, Version 5)
Name ARENA_GPC
Scope
Viruses; Arenaviridae
Templates P19240 (GLYC_MOPEI); P08669 (GLYC_LASSJ); P26313 (GLYC_JUNIN); P09991 (GLYC_LYCVA): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
GLYC
Protein name
RecName: Full=Pre-glycoprotein polyprotein GP complex;
Short=Pre-GP-C;
Contains:
RecName: Full=Stable signal peptide;
Short=SSP;
Contains:
RecName: Full=Glycoprotein G1;
Short=GP1;
Contains:
RecName: Full=Glycoprotein G2;
Short=GP2;
Gene name
GPC

Comments [?]

Function Stable signal peptide (SSP): cleaved and functions as a signal peptide. In addition, it is also retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of GP1 and GP2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.
Glycoprotein G1: interacts with the host receptor.
Glycoprotein G2: class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced upon acidification in the endosome.
Subunit [Glycoprotein G1]: homotetramer; disulfide-linked.
[Glycoprotein G2]: homotetramer. GP2 homotetramers bind through ionic interactions with GP1 homotetramers to form the GP complex together with the stable signal peptide. The GP-C polyprotein interacts with the host protease MBTPS1/SKI-1 resulting in the polyprotein processing.
Subcellular location Stable signal peptide: Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein. Host Golgi apparatus membrane; Multi-pass membrane protein. Host cell membrane; Multi-pass membrane protein.
Glycoprotein G1: Virion membrane; Peripheral membrane protein. Host endoplasmic reticulum membrane; Peripheral membrane protein. Host Golgi apparatus membrane; Peripheral membrane protein. Host cell membrane; Peripheral membrane protein.
Glycoprotein G2: Virion membrane; Single-pass membrane protein. Host endoplasmic reticulum membrane; Single-pass membrane protein. Host Golgi apparatus membrane; Single-pass membrane protein. Host cell membrane; Single-pass membrane protein. Note=Binding to the stable signal peptide masks endogenous ER localization signals in the cytoplasmic domain of G2 to ensure that only the fully assembled, tripartite GP complex is transported for virion assembly.
Domain The cytoplasmic domain of GP2 plays a role in ER location. It also contains a zinc-binding domain that allows SSP retention in the GPC complex by accepting a cysteine from SSP as the fourth ligand.
Ptm The SSP remains stably associated with the GP complex following cleavage by signal peptidase and plays crucial roles in the trafficking of GP through the secretory pathway.
Specific enzymatic cleavages in vivo yield mature proteins. GP-C polyprotein is cleaved in the endoplasmic reticulum by the host protease MBTPS1. Only cleaved glycoprotein is incorporated into virions.
Similarity Belongs to the arenaviridae GPC protein family.

Keywords [?]


Gene Ontology [?]

GO:0020002; Cellular component: host cell plasma membrane.
GO:0016021; Cellular component: integral component of membrane.
GO:0019031; Cellular component: viral envelope.
GO:0055036; Cellular component: virion membrane.
GO:0039654; Biological process: fusion of virus membrane with host endosome membrane.
GO:0019065; Biological process: receptor-mediated endocytosis of virus by host cell.
GO:0019062; Biological process: virion attachment to host cell.

Cross-references [?]

Pfam PF00798; Arena_glycoprot; 1;
PIRSF PIRSF004028; GPC_ArenaV; 1;
PROSITE PS00001; ASN_GLYCOSYLATION; 0-unlimited; trigger=PRU00498;

Computed features [?]

General Transmembrane; -; 3;

Features [?]

From: GLYC_JUNIN (P26313)
Key     From     To       Description   Tag   Condition   FTGroup
INIT_MET     1     1       Removed; by host     M  
CHAIN     2     Cter       Pre-glycoprotein polyprotein GP complex        
CHAIN     2     58       Stable signal peptide        
CHAIN     59     251       Glycoprotein G1        
CHAIN     252     Cter       Glycoprotein G2        
TOPO_DOM     2     17       Extracellular        
TRANSMEM     18     32       Helical        
TOPO_DOM     33     33       Cytoplasmic        
TRANSMEM     34     53       Helical        
TOPO_DOM     54     58       Extracellular        
TOPO_DOM     59     424       Extracellular        
TRANSMEM     425     445       Helical        
TOPO_DOM     446     Cter       Cytoplasmic        
METAL     57     57       Zinc 1     C  
METAL     447     447       Zinc 2; via tele nitrogen     H  
METAL     449     449       Zinc 2; via tele nitrogen     H  
METAL     455     455       Zinc 2     C  
METAL     459     459       Zinc 1; via pros nitrogen     H  
METAL     467     467       Zinc 1     C  
METAL     469     469       Zinc 1     C  
METAL     485     485       Zinc 2; via tele nitrogen     H  
SITE     58     59       Cleavage; by host signal peptidase        
SITE     251     252       Cleavage; by host MBTPS1        
LIPID     2     2       N-myristoyl glycine; by host     G  
DISULFID     92     226             C-x*-C  
DISULFID     135     164             C-x*-C  
DISULFID     207     213             C-x*-C  
From: GLYC_LASSJ (P26313)
DISULFID     118     155             C-x*-C  
DISULFID     180     212             C-x*-C  
DISULFID     279     292             C-x*-C  
DISULFID     301     310             C-x*-C  
DISULFID     364     385             C-x*-C  

Additional information [?]

Size range 479-518 amino acids
Related rules None
Fusion None