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Annotation rule MF_04086
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General rule information [?]

Accession MF_04086
Dates 8-FEB-2018 (Created)
20-NOV-2019 (Last updated, Version 4)
Name ARENA_L
Scope
Viruses; Arenaviridae
Template P14240 (L_LYCVA)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
L
Protein name
RecName: Full=RNA-directed RNA polymerase L;
Short=Protein L;
EC 2.7.7.48;
EC 3.1.-.-;
AltName: Full=Large structural protein;
AltName: Full=Replicase;
AltName: Full=Transcriptase;
Gene name
L

Comments [?]

Function RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner.
Catalytic activity RHEA:21248: a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
EC 2.7.7.48
Subunit Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N. Interacts with protein Z; this interaction inhibits viral transcription and replication.
Subcellular location Virion. Host cytoplasm.
Domain The N-terminal domain contains an RNA endonuclease activity that plays an essential role in mRNA transcription. This activity may be involved in cap snatching.
Similarity Belongs to the arenaviridae RNA polymerase family.

Keywords [?]

Cap snatching
Host cytoplasm
Hydrolase
Nucleotide-binding
Nucleotidyltransferase
RNA-directed RNA polymerase
Transferase
Viral RNA replication
Virion

Gene Ontology [?]

GO:0030430; Cellular component: host cell cytoplasm.
GO:0019012; Cellular component: virion.
GO:0000166; Molecular function: nucleotide binding.
GO:0003968; Molecular function: RNA-directed 5'-3' RNA polymerase activity.
GO:0075526; Biological process: cap snatching.
GO:0039689; Biological process: negative stranded viral RNA replication.
GO:0039696; Biological process: RNA-templated viral transcription.

Cross-references [?]

PROSITE PS50525; RDRP_SSRNA_NEG_SEG; 1;
Pfam PF06317; Arena_RNA_pol; 1;
PF17296; ArenaCapSnatch; 1;
PIRSF PIRSF000836; L_ArenaV; 1;

Features [?]

From: L_LYCVA (P14240)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     1166     1360       RdRp catalytic        

Additional information [?]

Size range 2198-2238 amino acids
Related rules None
Fusion None


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