![]() |
|
Annotation rule MF_04086 |
Accession | MF_04086 |
Dates | 8-FEB-2018 (Created) 20-NOV-2019 (Last updated, Version 4) |
Name | ARENA_L |
Scope | Viruses; Arenaviridae |
Template | P14240 (L_LYCVA) |
Triggered by |
Identifier |
|
Protein name |
|
Gene name |
|
Function | RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner. |
Catalytic activity | RHEA:21248: a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
EC 2.7.7.48 |
Subunit | Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N. Interacts with protein Z; this interaction inhibits viral transcription and replication. |
Subcellular location | Virion. Host cytoplasm. |
Domain | The N-terminal domain contains an RNA endonuclease activity that plays an essential role in mRNA transcription. This activity may be involved in cap snatching. |
Similarity | Belongs to the arenaviridae RNA polymerase family. |
PROSITE | PS50525; RDRP_SSRNA_NEG_SEG; 1; |
Pfam | PF06317; Arena_RNA_pol; 1; |
PF17296; ArenaCapSnatch; 1; | |
PIRSF | PIRSF000836; L_ArenaV; 1; |
From: L_LYCVA (P14240) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
DOMAIN | 1166 | 1360 | RdRp catalytic |
Size range | 2198-2238 amino acids |
Related rules | None |
Fusion | None |