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Annotation rule MF_04087
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General rule information [?]

Accession MF_04087
Dates 8-FEB-2018 (Created)
20-NOV-2019 (Last updated, Version 3)
Viruses; Arenaviridae
Template O73557 (Z_LASSJ)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=RING finger protein Z;
Short=Protein Z;
AltName: Full=Zinc-binding protein;
Gene name

Comments [?]

Function Plays a crucial role in virion assembly and budding. Expressed late in the virus life cycle, it acts as an inhibitor of viral transcription and RNA synthesis by interacting with the viral polymerase L. Presumably recruits the NP encapsidated genome to cellular membranes at budding sites via direct interaction with NP. Plays critical roles in the final steps of viral release by interacting with host TSG101, a member of the vacuolar protein-sorting pathway and using other cellular host proteins involved in vesicle formation pathway. The budding of the virus progeny occurs after association of protein Z with the viral glycoprotein complex SSP-GP1-GP2 at the cell periphery, step that requires myristoylation of protein Z. Also selectively represses protein production by associating with host eIF4E.
Subunit Interacts with protein NP; this interaction probably directs the encapsidated genome to budding sites. Interacts (via RING domain) with polymerase L; this interaction inhibits viral transcription and replication. Interacts with the glycoprotein complex; this interaction plays a role in virion budding. Interacts with host eIF4E; this interaction results in eIF4E reduced affinity for its substrate, the 5'-m7 G cap structure. Interacts (via late-budding domain) with host TSG101; this interaction is essential for budding and release of viral particles. Interacts with host RPLP0; this interaction may serve to load ribosome-like particles inside the virion. Interacts with host PML; this interaction induces PML bodies redistribution in the cytoplasm upon viral infection.
Subcellular location Virion. Host cytoplasm, host perinuclear region. Host cell membrane; Lipid-anchor; Cytoplasmic side. Note=Mainly perinuclear. During budding, associates at the inner side of the plasma membrane of infected cells.
Domain Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins.
Similarity Belongs to the arenaviridae Z protein family.

Keywords [?]

Gene Ontology [?]

GO:0044220; Cellular component: host cell perinuclear region of cytoplasm.
GO:0020002; Cellular component: host cell plasma membrane.
GO:0016020; Cellular component: membrane.
GO:0019012; Cellular component: virion.
GO:0003723; Molecular function: RNA binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0046761; Biological process: viral budding from plasma membrane.
GO:0039702; Biological process: viral budding via host ESCRT complex.

Cross-references [?]

Pfam PF03854; zf-P11; 1;
PIRSF PIRSF004030; Z_ArenaV; 1;

Features [?]

From: Z_LASSJ (O73557)
Key     From     To       Description   Tag   Condition   FTGroup
INIT_MET     1     1       Removed; by host     M  
CHAIN     2     Cter       RING finger protein Z        
ZN_FING     31     67       RING-type; atypical     C-x-x-C-x*-C-x-x-C  
MOTIF     81     84       PTAP/PSAP motif     P-[TS]-A-P  
MOTIF     94     97       PPXY motif     P-P-P-Y  
LIPID     2     2       N-myristoyl glycine; by host     G  

Additional information [?]

Size range 90-101 amino acids
Related rules None
Fusion None