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HAMAP rule MF_04089

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General rule information [?]

Accession MF_04089
Dates 9-FEB-2018 (Created)
29-AUG-2023 (Last updated, Version 7)
Scope(s) Viruses
Template(s) P03537 (NSP2_ROTS1); Q03243 (NSP2_ROTSR); [ Recover all ]
Triggered by HAMAP; MF_04089 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NSP2
Protein name RecName: Full=Non-structural protein 2;
AltName: Full=NCVP3;
AltName: Full=Non-structural RNA-binding protein 35;

Comments [?]

FUNCTIONParticipates in replication and packaging of the viral genome. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activities. The unwinding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. The RTPase activity plays a role in the removal of the gamma-phosphate from the rotavirus RNA minus strands of dsRNA genome segments. Participates in the selective exclusion of host proteins from stress granules (SG) and P bodies (PB). Participates also in the sequestration of these remodeled organelles in viral factories.
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SUBUNITHomooctamer. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 phosphorylation and formation of viral factories. Interacts with host DCP1A, DCP1B, DDX6, EDC4 and EIF2S1/eIF2-alpha; these interactions are probably part of the sequestration of some host SGs and PBs proteins in viral factories.
SUBCELLULAR LOCATIONHost cytoplasm. Note=Found in spherical cytoplasmic structures, called viral factories, that appear early after infection and are the site of viral replication and packaging.
SIMILARITYBelongs to the rotavirus NSP2 family.

Keywords [?]

Gene Ontology [?]

GO:0030430; Cellular component:host cell cytoplasm
GO:0016817; Molecular function:hydrolase activity, acting on acid anhydrides
GO:0046872; Molecular function:metal ion binding
GO:0000166; Molecular function:nucleotide binding
GO:0003723; Molecular function:RNA binding
GO:0019079; Biological process:viral genome replication

Cross-references [?]

Pfam PF02509; Rota_NS35; 1;

Features [?]

From: NSP2_ROTS1 (P03537)
Key From To Description Tag Condition FTGroup
REGION 205 241 /note="RNA-binding"
ACT_SITE 225 225 /note="For NTPase and RTPase activities" H
From: NSP2_ROTSR (Q03243)
Key From To Description Tag Condition FTGroup
BINDING 107 109 /ligand="ATP"
BINDING 221 223 /ligand="ATP"
BINDING 188 188 /ligand="ATP"
BINDING 227 227 /ligand="ATP"

Additional information [?]

Size range 279-317 amino acids
Related rules None
Fusion Nter: None Cter: None

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