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HAMAP rule MF_04089

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General rule information [?]

Accession MF_04089
Dates 9-FEB-2018 (Created)
19-NOV-2022 (Last updated, Version 6)
Viruses; Rotavirus
Templates P03537 (NSP2_ROTS1); Q03243 (NSP2_ROTSR): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Non-structural protein 2;
EC 3.6.4.-;
AltName: Full=NCVP3;
AltName: Full=Non-structural RNA-binding protein 35;

Comments [?]

Function Participates in replication and packaging of the viral genome. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activities. The unwinding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. The RTPase activity plays a role in the removal of the gamma-phosphate from the rotavirus RNA minus strands of dsRNA genome segments.
Cofactor Mg(2+)
Subunit Homooctamer. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 phosphorylation and formation of viral factories.
Subcellular location Host cytoplasm. Note=Found in spherical cytoplasmic structures, called viral factories, that appear early after infection and are the site of viral replication and packaging.
Similarity Belongs to the rotavirus NSP2 family.

Keywords [?]

Gene Ontology [?]

GO:0030430; Cellular component: host cell cytoplasm.
GO:0016817; Molecular function: hydrolase activity, acting on acid anhydrides.
GO:0046872; Molecular function: metal ion binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0003723; Molecular function: RNA binding.
GO:0019079; Biological process: viral genome replication.

Cross-references [?]

Pfam PF02509; Rota_NS35; 1;

Features [?]

From: NSP2_ROTS1 (P03537)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     205     241       RNA-binding        
ACT_SITE     225     225       For NTPase and RTPase activities     H  
From: NSP2_ROTSR (Q03243)
BINDING     107     109       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [SDQ]-[VI]-[RLF]  
BINDING     221     223       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     H-G-[KH]  
BINDING     188     188       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     K  
BINDING     227     227       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     R  

Additional information [?]

Size range 279-317 amino acids
Related rules None
Fusion None