HAMAP rule MF_04089
General rule information
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| Accession | MF_04089 |
| Dates | 9-FEB-2018 (Created)
29-AUG-2023 (Last updated, Version 7) |
| Name | ROTA_NSP2 |
| Scope(s) |
Viruses Rotavirus |
| Template(s) | P03537 (NSP2_ROTS1); Q03243 (NSP2_ROTSR); [ Recover all ] |
| Triggered by |
HAMAP; MF_04089 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | NSP2 |
| Protein name | RecName: Full=Non-structural protein 2; Short=NSP2; EC=3.6.4.-; AltName: Full=NCVP3; AltName: Full=Non-structural RNA-binding protein 35; Short=NS35; |
Comments
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| FUNCTION | Participates in replication and packaging of the viral genome. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activities. The unwinding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. The RTPase activity plays a role in the removal of the gamma-phosphate from the rotavirus RNA minus strands of dsRNA genome segments. Participates in the selective exclusion of host proteins from stress granules (SG) and P bodies (PB). Participates also in the sequestration of these remodeled organelles in viral factories. |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| SUBUNIT | Homooctamer. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 phosphorylation and formation of viral factories. Interacts with host DCP1A, DCP1B, DDX6, EDC4 and EIF2S1/eIF2-alpha; these interactions are probably part of the sequestration of some host SGs and PBs proteins in viral factories. |
| SUBCELLULAR LOCATION | Host cytoplasm. Note=Found in spherical cytoplasmic structures, called viral factories, that appear early after infection and are the site of viral replication and packaging. |
| SIMILARITY | Belongs to the rotavirus NSP2 family. |
Keywords
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Gene Ontology
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| GO:0030430; Cellular component:host cell cytoplasm |
| GO:0016817; Molecular function:hydrolase activity, acting on acid anhydrides |
| GO:0046872; Molecular function:metal ion binding |
| GO:0000166; Molecular function:nucleotide binding |
| GO:0003723; Molecular function:RNA binding |
| GO:0019079; Biological process:viral genome replication |
Cross-references
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| Pfam | PF02509; Rota_NS35; 1; |
Features
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| From: NSP2_ROTS1 (P03537) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 205 | 241 | /note="RNA-binding" | |||||||||
| ACT_SITE | 225 | 225 | /note="For NTPase and RTPase activities" | H | ||||||||
| From: NSP2_ROTSR (Q03243) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 107 | 109 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[SDQ]-[VI]-[RLF] | ||||||||
| BINDING | 221 | 223 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
H-G-[KH] | ||||||||
| BINDING | 188 | 188 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
| BINDING | 227 | 227 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R | ||||||||
Additional information
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| Size range | 279-317 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |