HAMAP rule MF_04089

General rule information [?]

Accession	MF_04089
Dates	9-FEB-2018 (Created) 19-NOV-2022 (Last updated, Version 6)

Name

ROTA_NSP2

Scope

Viruses; Rotavirus

Templates

P03537 (NSP2_ROTS1); Q03243 (NSP2_ROTSR): [Recover all]

Triggered by

HAMAP; MF_04089 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

NSP2

Protein name

RecName:		Full=Non-structural protein 2;
		Short=NSP2;
		EC 3.6.4.-;
AltName:		Full=NCVP3;
AltName:		Full=Non-structural RNA-binding protein 35;
		Short=NS35;

Comments [?]

Function	Participates in replication and packaging of the viral genome. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activities. The unwinding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. The RTPase activity plays a role in the removal of the gamma-phosphate from the rotavirus RNA minus strands of dsRNA genome segments.
Cofactor	Mg(2+)
Subunit	Homooctamer. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 phosphorylation and formation of viral factories.
Subcellular location	Host cytoplasm. Note=Found in spherical cytoplasmic structures, called viral factories, that appear early after infection and are the site of viral replication and packaging.
Similarity	Belongs to the rotavirus NSP2 family.

Keywords [?]

ATP-binding
Host cytoplasm
Hydrolase
Magnesium
Metal-binding
Nucleotide-binding
RNA-binding

Gene Ontology [?]

GO:0030430; Cellular component: host cell cytoplasm.
GO:0016817; Molecular function: hydrolase activity, acting on acid anhydrides.
GO:0046872; Molecular function: metal ion binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0003723; Molecular function: RNA binding.
GO:0019079; Biological process: viral genome replication.

Cross-references [?]

Pfam	PF02509; Rota_NS35; 1;

Features [?]

From: NSP2_ROTS1 (P03537)

Key From To Description Tag Condition FTGroup

REGION 205 241 RNA-binding

ACT_SITE 225 225 For NTPase and RTPase activities H

From: NSP2_ROTSR (Q03243)

BINDING 107 109 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [SDQ]-[VI]-[RLF]

BINDING 221 223 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 H-G-[KH]

BINDING 188 188 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 K

BINDING 227 227 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 R

Additional information [?]

Size range	279-317 amino acids
Related rules	None
Fusion	None

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