HAMAP rule MF_04092
General rule information
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| Accession | MF_04092 |
| Dates | 2-MAR-2018 (Created)
29-AUG-2023 (Last updated, Version 8) |
| Name | ROTA_NSP5 |
| Scope(s) |
Viruses Rotavirus |
| Template(s) | A2T3Q9 (NSP5_ROTSH); [ Recover all ] |
| Triggered by |
HAMAP; MF_04092 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | NSP5 |
| Protein name | RecName: Full=Non-structural protein 5; Short=NSP5; AltName: Full=NS26; |
Comments
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| FUNCTION | Plays an essential role in the viral genome replication. Participates, together with NSP2, in the formation of viral factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Orchestrates the recruitment of viroplasmic proteins such as capsid proteins to these factories. Participates in the selective exclusion of host proteins from stress granules (SG) and P bodies (PB). Participates also in the sequestration of these remodeled organelles in viral factories. |
| case <FTTag:Metal> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| end case | |
| case <FTGroup:1> | |
| SUBUNIT | Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6. Interacts with host DCP1A, DCP1B, DDX6, EDC4, EIF2S1/eIF2-alpha, AGO2 and CAPRIN1; these interactions are probably part of the sequestration of some host SGs and PBs proteins in viral factories. |
| else | |
| SUBUNIT | Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2 and NSP6. |
| end case | |
| SUBCELLULAR LOCATION | Host cytoplasm. Note=Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging. |
| PTM | O-glycosylated. |
| case <FTGroup:1> | |
| PTM | Hyperphosphorylated on serine residues, when in dimeric form. Phosphorylation by host CK1 is required for the hyperphosphorylation of NSP5 dimer. |
| end case | |
| SIMILARITY | Belongs to the rotavirus NSP5 family. |
Keywords
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| Glycoprotein | |
| Host cytoplasm | |
| case <FTTag:Metal> | |
| Magnesium | |
| Metal-binding | |
| end case | |
| Nucleotide-binding | |
| RNA-binding | |
| case <FTGroup:1> | |
| Phosphoprotein | |
| end case | |
Gene Ontology
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| GO:0030430; Cellular component:host cell cytoplasm |
| GO:0016887; Molecular function:ATP hydrolysis activity |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0000166; Molecular function:nucleotide binding |
| GO:0003723; Molecular function:RNA binding |
| GO:0019079; Biological process:viral genome replication |
Cross-references
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| Pfam | PF01525; Rota_NS26; 1; |
| Pfam | PF17580; GBR_NSP5; 1; |
| PIRSF | PIRSF004006; Rota_NS26; 1; |
Features
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| From: NSP5_ROTSH (A2T3Q9) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 92 | 92 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
Metal | D | |||||||
| MOD_RES | 67 | 67 | /note="Phosphoserine; by host CK1" | S | 1 | |||||||
| MOD_RES | 154 | 154 | /note="Phosphoserine; by host" | S | 1 | |||||||
| MOD_RES | 156 | 156 | /note="Phosphoserine; by host" | S | 1 | |||||||
| MOD_RES | 164 | 164 | /note="Phosphoserine; by host" | S | 1 | |||||||
| MOD_RES | 166 | 166 | /note="Phosphoserine; by host" | S | 1 | |||||||
Additional information
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| Size range | 170-212 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |