HAMAP logo

HAMAP rule MF_04092

Send feedback

General rule information [?]

Accession MF_04092
Dates 2-MAR-2018 (Created)
29-AUG-2023 (Last updated, Version 8)
Scope(s) Viruses
Template(s) A2T3Q9 (NSP5_ROTSH); [ Recover all ]
Triggered by HAMAP; MF_04092 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NSP5
Protein name RecName: Full=Non-structural protein 5;
AltName: Full=NS26;

Comments [?]

FUNCTIONPlays an essential role in the viral genome replication. Participates, together with NSP2, in the formation of viral factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Orchestrates the recruitment of viroplasmic proteins such as capsid proteins to these factories. Participates in the selective exclusion of host proteins from stress granules (SG) and P bodies (PB). Participates also in the sequestration of these remodeled organelles in viral factories.
case <FTTag:Metal>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
end case
case <FTGroup:1>
SUBUNITHomodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6. Interacts with host DCP1A, DCP1B, DDX6, EDC4, EIF2S1/eIF2-alpha, AGO2 and CAPRIN1; these interactions are probably part of the sequestration of some host SGs and PBs proteins in viral factories.
SUBUNITHomodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2 and NSP6.
end case
SUBCELLULAR LOCATIONHost cytoplasm. Note=Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.
case <FTGroup:1>
PTMHyperphosphorylated on serine residues, when in dimeric form. Phosphorylation by host CK1 is required for the hyperphosphorylation of NSP5 dimer.
end case
SIMILARITYBelongs to the rotavirus NSP5 family.

Keywords [?]

Host cytoplasm
case <FTTag:Metal>
end case
case <FTGroup:1>
end case

Gene Ontology [?]

GO:0030430; Cellular component:host cell cytoplasm
GO:0016887; Molecular function:ATP hydrolysis activity
GO:0000287; Molecular function:magnesium ion binding
GO:0000166; Molecular function:nucleotide binding
GO:0003723; Molecular function:RNA binding
GO:0019079; Biological process:viral genome replication

Cross-references [?]

Pfam PF01525; Rota_NS26; 1;
Pfam PF17580; GBR_NSP5; 1;
PIRSF PIRSF004006; Rota_NS26; 1;

Features [?]

From: NSP5_ROTSH (A2T3Q9)
Key From To Description Tag Condition FTGroup
BINDING 92 92 /ligand="Mg(2+)"
Metal D
MOD_RES 67 67 /note="Phosphoserine; by host CK1" S 1
MOD_RES 154 154 /note="Phosphoserine; by host" S 1
MOD_RES 156 156 /note="Phosphoserine; by host" S 1
MOD_RES 164 164 /note="Phosphoserine; by host" S 1
MOD_RES 166 166 /note="Phosphoserine; by host" S 1

Additional information [?]

Size range 170-212 amino acids
Related rules None
Fusion Nter: None Cter: None

View rule in raw text format (no links)