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HAMAP rule MF_04092

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General rule information [?]

Accession MF_04092
Dates 2-MAR-2018 (Created)
19-NOV-2022 (Last updated, Version 7)
Name ROTA_NSP5
Scope
Viruses; Rotavirus
Template A2T3Q9 (NSP5_ROTSH)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NSP5
Protein name
RecName: Full=Non-structural protein 5;
Short=NSP5;
AltName: Full=NS26;

Comments [?]

Function Plays an essential role in the viral genome replication. Participates, together with NSP2, in the formation of viral factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Orchestrates the recruitment of viroplasmic proteins such as capsid proteins to these factories.
case <FTTag:Metal>
Cofactor Mg(2+)
end case
case <FTGroup:1>
Subunit Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6.
else
Subunit Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2 and NSP6.
end case
Subcellular location Host cytoplasm. Note=Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.
Ptm O-glycosylated.
case <FTGroup:1>
Ptm Hyperphosphorylated on serine residues, when in dimeric form. Phosphorylation by host CK1 is required for the hyperphosphorylation of NSP5 dimer.
end case
Similarity Belongs to the rotavirus NSP5 family.

Keywords [?]

Glycoprotein
Host cytoplasm
case <FTTag:Metal>
Magnesium
Metal-binding
end case
Nucleotide-binding
RNA-binding
case <FTGroup:1>
Phosphoprotein
end case

Gene Ontology [?]

GO:0030430; Cellular component: host cell cytoplasm.
GO:0016887; Molecular function: ATP hydrolysis activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0003723; Molecular function: RNA binding.
GO:0019079; Biological process: viral genome replication.

Cross-references [?]

Pfam PF01525; Rota_NS26; 1;
PF17580; GBR_NSP5; 1;
PIRSF PIRSF004006; Rota_NS26; 1;

Features [?]

From: NSP5_ROTSH (A2T3Q9)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     92     92       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420   Metal   D  
MOD_RES     67     67       Phosphoserine; by host CK1     S   1
MOD_RES     154     154       Phosphoserine; by host     S   1
MOD_RES     156     156       Phosphoserine; by host     S   1
MOD_RES     164     164       Phosphoserine; by host     S   1
MOD_RES     166     166       Phosphoserine; by host     S   1

Additional information [?]

Size range 170-212 amino acids
Related rules None
Fusion None


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