HAMAP rule MF_04124
General rule information
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Accession | MF_04124 |
Dates | 28-MAR-2018 (Created)
30-JAN-2024 (Last updated, Version 12) |
Name | Rota_VP3 |
Scope(s) |
Viruses Rotavirus |
Template(s) | A2T3S5 (VP3_ROTSH); Q6WAT6 (VP3_ROTBU); [ Recover all ] |
Triggered by |
HAMAP; MF_04124 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | VP3 |
Protein name | RecName: Full=Protein VP3; Includes: RecName: Full=mRNA guanylyltransferase; EC=2.7.7.50; Includes: RecName: Full=mRNA (guanine-N(7))-methyltransferase; EC=2.1.1.56; |
Comments
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FUNCTION | Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms a VP1-VP3 complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores do not. |
CATALYTIC ACTIVITY | Reaction=a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'- end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate; Xref=Rhea:RHEA:67012, Rhea:RHEA-COMP:17165, Rhea:RHEA-COMP:17166, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:167616, ChEBI:CHEBI:167617; EC=2.7.7.50; |
CATALYTIC ACTIVITY | Reaction=a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S- adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'- triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:67008, Rhea:RHEA-COMP:17166, Rhea:RHEA- COMP:17167, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461, ChEBI:CHEBI:167617; EC=2.1.1.56; |
SUBUNIT | Interacts with VP1. Interacts with VP2. |
SUBCELLULAR LOCATION | Virion. Note=Attached inside the inner capsid as a minor component. There are about 11 to 12 copies per virion. |
SIMILARITY | Belongs to the rotavirus VP3 family. |
Keywords
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Gene Ontology
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GO:0019013; Cellular component:viral nucleocapsid |
GO:0005525; Molecular function:GTP binding |
GO:0004482; Molecular function:mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
GO:0004484; Molecular function:mRNA guanylyltransferase activity |
GO:0003723; Molecular function:RNA binding |
GO:0016032; Biological process:viral process |
Cross-references
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PROSITE | PS51589; SAM_MT56_VP3; 1; |
Pfam | PF06929; Rotavirus_VP3; 1; |
PIRSF | PIRSF004015; LigT_rotavirus; 1; |
Features
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From: VP3_ROTSH (A2T3S5) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 171 | 245 | /note="N7-methyltransferase activity" | |||||||||
REGION | 246 | 428 | /note="2'-O-methyltransferase activity" | |||||||||
REGION | 429 | 555 | /note="N7-methyltransferase activity" | |||||||||
REGION | 556 | Cter | /note="GTase/RTPase activity" |
Additional information
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Size range | 690-840 amino acids |
Related rules |
MF_04128 |
Fusion | Nter: None Cter: None |