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Annotation rule MF_04124 |
Accession | MF_04124 |
Dates | 28-MAR-2018 (Created) 8-SEP-2020 (Last updated, Version 8) |
Name | Rota_VP3 |
Scope | Viruses; Rotavirus |
Templates | A2T3S5 (VP3_ROTSH); Q6WAT6 (VP3_ROTBU); B3F2X4 (B3F2X4_ROTRH): [Recover all] |
Triggered by |
Identifier |
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Protein name |
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Function | Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms a VP1-VP3 complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores do not. |
Catalytic activity | RHEA:54592: a 5'-end diphospho-(purine-ribonucleoside) in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-(purine-ribonucleoside) in mRNA + diphosphate
EC 2.7.7.50 |
RHEA:60856: a 5'-end (5'-triphosphoguanosine)-guanosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-guanosine in mRNA + S-adenosyl-L-homocysteine
EC 2.1.1.56 |
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RHEA:60852: a 5'-end (5'-triphosphoguanosine)-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-adenosine in mRNA + S-adenosyl-L-homocysteine
EC 2.1.1.56 |
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Subunit | Interacts with VP1. Interacts with VP2. |
Subcellular location | Virion. Note=Attached inside the inner capsid as a minor component. There are about 11 to 12 copies per virion. |
Similarity | Belongs to the rotavirus VP3 family. |
PROSITE | PS51589; SAM_MT56_VP3; 1; |
Pfam | PF06929; Rotavirus_VP3; 1; |
PIRSF | PIRSF004015; LigT_rotavirus; 1; |
From: VP3_ROTSH (A2T3S5) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 171 | 245 | N7-methyltransferase activity | |||||||||
REGION | 246 | 428 | 2'-O-methyltransferase activity | |||||||||
REGION | 429 | 555 | N7-methyltransferase activity | |||||||||
REGION | 556 | Cter | GTase/RTPase activity |
Size range | 690-840 amino acids |
Related rules | MF_04128 (VP3 supersedes the current rule) |
Fusion | None |