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HAMAP rule MF_04131

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General rule information [?]

Accession MF_04131
Dates 28-MAR-2018 (Created)
19-NOV-2022 (Last updated, Version 6)
Name Rota_VP7_A
Scope(s) Viruses
Template(s) A2T3P5 (VP7_ROTSH); P12476 (VP7_ROTRH); Q86515 (VP7_ROTRF); P03533 (VP7_ROTS1); [ Recover all ]
Triggered by HAMAP; MF_04131 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier VP7
Protein name RecName: Full=Outer capsid glycoprotein VP7;
                 Flags: Precursor;

Comments [?]

FUNCTIONCalcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.
SUBUNITHomotrimer; disulfide-linked. 2 Ca(2+) ions bound at each subunit interface in the trimer hold the trimer together. Interacts with the intermediate capsid protein VP6. Interacts with the outer capsid protein VP5*.
SUBCELLULAR LOCATIONVirion. Host endoplasmic reticulum lumen. Note=The outer layer contains 780 copies of VP7, grouped as 260 trimers. Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles.
PTMThe N-terminus is blocked possibly by pyroglutamic acid.
MISCELLANEOUSSome rotavirus strains are neuraminidase-sensitive and require sialic acid to attach to the cell surface. Some rotavirus strains are integrin-dependent. Some rotavirus strains depend on ganglioside for their entry into the host cell. Hsp70 also seems to be involved in the entry of some strains.
MISCELLANEOUSIn group A rotaviruses, VP7 defines the G serotype.
SIMILARITYBelongs to the rotavirus VP7 family.

Keywords [?]

Gene Ontology [?]

GO:0044166; Cellular component:host cell endoplasmic reticulum lumen
GO:0039621; Cellular component:T=13 icosahedral viral capsid
GO:0039624; Cellular component:viral outer capsid

Cross-references [?]

Pfam PF00434; VP7; 1;
General Signal; -; 1;

Features [?]

From: VP7_ROTSH (A2T3P5)
Key From To Description Tag Condition FTGroup
SIGNAL Nter 50
CHAIN 51 Cter /note="Outer capsid glycoprotein VP7"
BINDING 95 95 /ligand="Ca(2+)"
BINDING 206 206 /ligand="Ca(2+)"
BINDING 214 214 /ligand="Ca(2+)"
BINDING 216 216 /ligand="Ca(2+)"
BINDING 177 177 /ligand="Ca(2+)"
BINDING 228 228 /ligand="Ca(2+)"
BINDING 229 229 /ligand="Ca(2+)"
BINDING 231 231 /ligand="Ca(2+)"
BINDING 301 301 /ligand="Ca(2+)"
DISULFID 82 135 C-x*-C
DISULFID 165 249 C-x*-C
DISULFID 191 244 C-x*-C
DISULFID 196 207 C-x*-C
REGION 237 239 /note="LVD motif; interaction with ITGA4/ITGB1 heterodimer" L-D-V
REGION 253 255 /note="GPR motif; interaction with ITGAX/ITGB2" G-P-R
REGION 165 167 /note="CNP motif; interaction with ITGAV/ITGB3" C-N-P

Additional information [?]

Size range 300-331 amino acids
Related rules None
Fusion Nter: None Cter: None

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