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HAMAP rule MF_04131

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General rule information [?]

Accession MF_04131
Dates 28-MAR-2018 (Created)
19-NOV-2022 (Last updated, Version 6)
Name Rota_VP7_A
Viruses; Rotavirus
Templates A2T3P5 (VP7_ROTSH); P12476 (VP7_ROTRH); Q86515 (VP7_ROTRF); P03533 (VP7_ROTS1): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Outer capsid glycoprotein VP7;
Flags: Precursor;

Comments [?]

Function Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.
Subunit Homotrimer; disulfide-linked. 2 Ca(2+) ions bound at each subunit interface in the trimer hold the trimer together. Interacts with the intermediate capsid protein VP6. Interacts with the outer capsid protein VP5*.
Subcellular location Virion. Host endoplasmic reticulum lumen. Note=The outer layer contains 780 copies of VP7, grouped as 260 trimers. Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles.
Ptm N-glycosylated.
The N-terminus is blocked possibly by pyroglutamic acid.
Miscellaneous Some rotavirus strains are neuraminidase-sensitive and require sialic acid to attach to the cell surface. Some rotavirus strains are integrin-dependent. Some rotavirus strains depend on ganglioside for their entry into the host cell. Hsp70 also seems to be involved in the entry of some strains.
In group A rotaviruses, VP7 defines the G serotype.
Similarity Belongs to the rotavirus VP7 family.

Keywords [?]

Gene Ontology [?]

GO:0044166; Cellular component: host cell endoplasmic reticulum lumen.
GO:0039621; Cellular component: T=13 icosahedral viral capsid.
GO:0039624; Cellular component: viral outer capsid.

Cross-references [?]

Pfam PF00434; VP7; 1;

Computed features [?]

General Signal; -; 1;

Features [?]

From: VP7_ROTSH (A2T3P5)
Key     From     To       Description   Tag   Condition   FTGroup
SIGNAL     Nter     50                
CHAIN     51     Cter       Outer capsid glycoprotein VP7        
BINDING     95     95       /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="1     D  
BINDING     206     206       /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="1     G  
BINDING     214     214       /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="1     [TS]  
BINDING     216     216       /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="1     E  
BINDING     177     177       /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="2     Q  
BINDING     228     228       /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="2     D  
BINDING     229     229       /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="2     V  
BINDING     231     231       /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="2     D  
BINDING     301     301       /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" /ligand_label="2     D  
DISULFID     82     135             C-x*-C  
DISULFID     165     249             C-x*-C  
DISULFID     191     244             C-x*-C  
DISULFID     196     207             C-x*-C  
REGION     237     239       LVD motif; interaction with ITGA4/ITGB1 heterodimer     L-D-V  
REGION     253     255       GPR motif; interaction with ITGAX/ITGB2     G-P-R  
REGION     165     167       CNP motif; interaction with ITGAV/ITGB3     C-N-P  

Additional information [?]

Size range 300-331 amino acids
Related rules None
Fusion None