HAMAP rule MF_04131

General rule information [?]

Accession	MF_04131
Dates	28-MAR-2018 (Created) 19-NOV-2022 (Last updated, Version 6)
Name	Rota_VP7_A
Scope(s)	Viruses Rotavirus
Template(s)	A2T3P5 (VP7_ROTSH); P12476 (VP7_ROTRH); Q86515 (VP7_ROTRF); P03533 (VP7_ROTS1); [ Recover all ]
Triggered by	HAMAP; MF_04131 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	VP7
Protein name	RecName: Full=Outer capsid glycoprotein VP7; Flags: Precursor;

Comments [?]

FUNCTION	Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.
SUBUNIT	Homotrimer; disulfide-linked. 2 Ca(2+) ions bound at each subunit interface in the trimer hold the trimer together. Interacts with the intermediate capsid protein VP6. Interacts with the outer capsid protein VP5*.
SUBCELLULAR LOCATION	Virion. Host endoplasmic reticulum lumen. Note=The outer layer contains 780 copies of VP7, grouped as 260 trimers. Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles.
PTM	N-glycosylated.
PTM	The N-terminus is blocked possibly by pyroglutamic acid.
MISCELLANEOUS	Some rotavirus strains are neuraminidase-sensitive and require sialic acid to attach to the cell surface. Some rotavirus strains are integrin-dependent. Some rotavirus strains depend on ganglioside for their entry into the host cell. Hsp70 also seems to be involved in the entry of some strains.
MISCELLANEOUS	In group A rotaviruses, VP7 defines the G serotype.
SIMILARITY	Belongs to the rotavirus VP7 family.

Keywords [?]

Host endoplasmic reticulum

Host-virus interaction

Metal-binding

Outer capsid protein

Signal

T=13 icosahedral capsid protein

Virion

Gene Ontology [?]

GO:0044166; Cellular component:host cell endoplasmic reticulum lumen

GO:0039621; Cellular component:T=13 icosahedral viral capsid

GO:0039624; Cellular component:viral outer capsid

Cross-references [?]

Pfam	PF00434; VP7; 1;
General	Signal; -; 1;

Features [?]

From: VP7_ROTSH (A2T3P5)

Key

From

Description

Tag

Condition

FTGroup

SIGNAL

Nter

CHAIN

Cter

/note="Outer capsid glycoprotein VP7"

BINDING

/ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="1"

BINDING

206

/ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="1"

BINDING

214

/ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="1"

[TS]

BINDING

216

/ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="1"

BINDING

177