HAMAP rule MF_04136
General rule information
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Accession | MF_04136 |
Dates | 9-APR-2019 (Created)
9-AUG-2022 (Last updated, Version 6) |
Name | SAR_ENDOLYSIN |
Scope(s) |
Viruses |
Template(s) | Q7Y2C0 (ENLYS_BPKMV); P27359 (ENLYS_BPP21); Q37875 (ENLYS_BPP1); [ Recover all ] |
Triggered by |
HAMAP; MF_04136 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | ENLYS |
Protein name | RecName: Full=SAR-endolysin; EC=3.2.1.17; AltName: Full=Endolysin; AltName: Full=Lysis protein; AltName: Full=Lysozyme; AltName: Full=Muramidase; |
Comments
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case <OC:Muvirus> | |
FUNCTION | Signal-arrest-release (SAR) endolysin with lysozyme activity that degrades host peptidoglycans and participates with the releasin and spanin proteins in the sequential events which lead to programmed host cell lysis releasing the mature viral particles. The SAR-endolysin is released by the releasin protein into the periplasm, where it breaks down the peptidoglycan layer. |
else | |
FUNCTION | Signal-arrest-release (SAR) endolysin with lysozyme activity that degrades host peptidoglycans and participates with the pinholin and spanin proteins in the sequential events which lead to programmed host cell lysis releasing the mature viral particles. Once the pinholin has permeabilized the host cell membrane, the SAR-endolysin is released into the periplasm where it breaks down the peptidoglycan layer. |
end case | |
CATALYTIC ACTIVITY | Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17; |
case <AnyFeature:Transmembrane<2> | |
SUBCELLULAR LOCATION | Host cell inner membrane; Single-pass type II membrane protein; Periplasmic side. Note=Secreted as a signal-anchored, membrane-tethered, inactive endolysin which is subsequently refolded, activated and released by membrane depolarization driven by the pinholin. |
DOMAIN | The signal-anchor, which may also be an uncleaved signal sequence tethers the SAR-endolysin to the membrane until the latter is depolarized by the holin, resulting in the escape of SAR-endolysin from the membrane. |
end case | |
case <FTGroup:1> | |
PTM | All the periplasmic cyteines of the inactive, membrane-associated endolysin are involved in disulfide bond. In the active soluble form, disulfide bonds are isomerized and only the catalytic cysteine remains free. |
end case | |
case not <FTTag:Act_site> and not <FTTag:Act_site_Cys> | |
CAUTION | Lacks the conserved Asp active site. |
end case | |
SIMILARITY | Belongs to the glycosyl hydrolase 24 family. |
Keywords
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Gene Ontology
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GO:0003796; Molecular function:lysozyme activity |
GO:0044659; Biological process:viral release from host cell by cytolysis |
GO:0009253; Biological process:peptidoglycan catabolic process |
Cross-references
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Pfam | PF00959; Phage_lysozyme; 1; |
General | Transmembrane; -; 1; |
Features
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From: ENLYS_BPKMV (Q7Y2C0) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
case not <AnyFeature:Transmembrane> | ||||||||||||
TRANSMEM | 1 | 19 | /note="Helical; Signal-anchor for type II membrane protein" | |||||||||
end case | ||||||||||||
ACT_SITE | 21 | 21 | /note="Proton donor/acceptor" | E | ||||||||
ACT_SITE | 30 | 30 | /note="Proton donor/acceptor" | Act_site | D | |||||||
From: ENLYS_BPP1 (Q37875) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 51 | 51 | /note="Proton donor/acceptor" | Act_site_Cys | C | |||||||
DISULFID | 13 | 44 | /note="In the active soluble endolysin; alternate" | C-x*-C | 1 | |||||||
DISULFID | 44 | 51 | /note="In the inactive membrane-associated endolysin; alternate" | C-x*-C | 1 |
Additional information
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Size range | 140-250 amino acids |
Related rules |
MF_04110 |
Fusion | Nter: None Cter: None |