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HAMAP rule MF_04136

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General rule information [?]

Accession MF_04136
Dates 9-APR-2019 (Created)
9-AUG-2022 (Last updated, Version 6)
Name SAR_ENDOLYSIN
Scope
Viruses
Templates Q7Y2C0 (ENLYS_BPKMV); P27359 (ENLYS_BPP21); Q37875 (ENLYS_BPP1): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ENLYS
Protein name
RecName: Full=SAR-endolysin;
EC 3.2.1.17;
AltName: Full=Endolysin;
AltName: Full=Lysis protein;
AltName: Full=Lysozyme;
AltName: Full=Muramidase;

Comments [?]

case <OC:Muvirus>
Function Signal-arrest-release (SAR) endolysin with lysozyme activity that degrades host peptidoglycans and participates with the releasin and spanin proteins in the sequential events which lead to programmed host cell lysis releasing the mature viral particles. The SAR-endolysin is released by the releasin protein into the periplasm, where it breaks down the peptidoglycan layer.
else
Function Signal-arrest-release (SAR) endolysin with lysozyme activity that degrades host peptidoglycans and participates with the pinholin and spanin proteins in the sequential events which lead to programmed host cell lysis releasing the mature viral particles. Once the pinholin has permeabilized the host cell membrane, the SAR-endolysin is released into the periplasm where it breaks down the peptidoglycan layer.
end case
Catalytic activity Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
case <AnyFeature:Transmembrane<2>
Subcellular location Host cell inner membrane; Single-pass type II membrane protein; Periplasmic side. Note=Secreted as a signal-anchored, membrane-tethered, inactive endolysin which is subsequently refolded, activated and released by membrane depolarization driven by the pinholin.
Domain The signal-anchor, which may also be an uncleaved signal sequence tethers the SAR-endolysin to the membrane until the latter is depolarized by the holin, resulting in the escape of SAR-endolysin from the membrane.
end case
case <FTGroup:1>
Ptm All the periplasmic cyteines of the inactive, membrane-associated endolysin are involved in disulfide bond. In the active soluble form, disulfide bonds are isomerized and only the catalytic cysteine remains free.
end case
case not <FTTag:Act_site> and not <FTTag:Act_site_Cys>
Caution Lacks the conserved Asp active site.
end case
Similarity Belongs to the glycosyl hydrolase 24 family.

Keywords [?]

Antimicrobial
Bacteriolytic enzyme
Cytolysis
Glycosidase
Host cell inner membrane
Host cell lysis by virus
Host cell membrane
Host membrane
Hydrolase
Membrane
Signal-anchor
Transmembrane
Transmembrane helix
Viral release from host cell
case <FTGroup:1>
Disulfide bond
end case

Gene Ontology [?]

GO:0003796; Molecular function: lysozyme activity.
GO:0044659; Biological process: viral release from host cell by cytolysis.
GO:0009253; Biological process: peptidoglycan catabolic process.

Cross-references [?]

Pfam PF00959; Phage_lysozyme; 1;

Computed features [?]

case <AnyFeature:Transmembrane<2>
General Transmembrane; -; 1; trigger=yes;
end case

Features [?]

case not <AnyFeature:Transmembrane>
From: ENLYS_BPKMV (Q7Y2C0)
Key     From     To       Description   Tag   Condition   FTGroup
TRANSMEM     1     19       Helical; Signal-anchor for type II membrane protein        
end case
ACT_SITE (Optional)     21     21       Proton donor/acceptor     E  
ACT_SITE (Optional)     30     30       Proton donor/acceptor   Act_site   D  
From: ENLYS_BPP1 (Q37875)
ACT_SITE (Optional)     51     51       Proton donor/acceptor   Act_site_Cys   C  
DISULFID     13     44       In the active soluble endolysin; alternate     C-x*-C   1
DISULFID     44     51       In the inactive membrane-associated endolysin; alternate     C-x*-C   1

Additional information [?]

Size range 140-250 amino acids
Related rules MF_04110 (ENLYS)
Fusion None


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