HAMAP rule MF_04145

General rule information [?]

Accession	MF_04145
Dates	17-JAN-2020 (Created) 1-JUN-2023 (Last updated, Version 9)
Name	TERL_SPP1
Scope(s)	Viruses Caudoviricetes
Template(s)	P54308 (TERL_BPSPP); [ Recover all ]
Triggered by	HAMAP; MF_04145 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	TERL
Protein name	RecName: Full=Terminase, large subunit; AltName: Full=DNA-packaging protein; Includes: RecName: Full=Endonuclease; EC=3.1.-.-; Includes: RecName: Full=ATPase; EC=3.6.4.-;

Comments [?]

FUNCTION	The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer once the capsid is full (headful packaging). Once the capsid is packaged with the DNA, the terminase complex is substituted by the adapter and the stopper protein that form the connector.
case <FTGroup:1>
COFACTOR	Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 divalent metal cations per subunit. Mn(2+) is preferred over Mg(2+).;
end case
SUBUNIT	Monomer. Interacts with the terminase small subunit; the active complex is probably heterooligomeric. Interacts with the portal protein.
DOMAIN	The N-terminus contains an ATPase domain and the C-terminus contains an endonuclease domain.

Keywords [?]

Gene Ontology [?]

GO:0098009; Cellular component:viral terminase, large subunit

GO:0004519; Molecular function:endonuclease activity

GO:0046872; Molecular function:metal ion binding

GO:0004518; Molecular function:nuclease activity

GO:0051276; Biological process:chromosome organization

GO:0019073; Biological process:viral DNA genome packaging

Cross-references [?]

Features [?]

From: TERL_BPSPP (P54308)
Key	From	To	Description	Tag	Condition	FTGroup
REGION	1	198	/note="ATPase activity"
REGION	232	422	/note="Nuclease activity and binding to the portal protein"
MOTIF	33	40	/note="Walker A motif"		G-[GS]-[AR]-[AG]-S-[AGK]-[AGK]-S
MOTIF	130	135	/note="Walker B motif"		x-[CAGLM]-W-[IFY]-E-E
ACT_SITE	135	135	/note="For ATPase activity"		E
BINDING	266	266	/ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1" /ligand_note="catalytic; for nuclease activity"		D	1
BINDING	266	266	/ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2" /ligand_note="catalytic; for nuclease activity"		D	1
BINDING	321	321	/ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1" /ligand_note="catalytic; for nuclease activity"		D	1
BINDING	400	400	/ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2" /ligand_note="catalytic; for nuclease activity"		H	1
BINDING	403	403	/ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2" /ligand_note="catalytic; for nuclease activity"		D	1

Additional information [?]