HAMAP rule MF_04147

General rule information [?]

Accession	MF_04147
Dates	7-FEB-2020 (Created) 19-NOV-2022 (Last updated, Version 6)
Name	TERL_T7
Scope(s)	Viruses Caudoviricetes
Template(s)	P03694 (TERL_BPT7); P10310 (TERL_BPT3); [ Recover all ]
Triggered by	HAMAP; MF_04147 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	TERL
Protein name	RecName: Full=Terminase, large subunit; AltName: Full=DNA-packaging protein; Includes: RecName: Full=ATPase; EC=3.6.4.-; Includes: RecName: Full=Endonuclease; EC=3.1.21.-;

Comments [?]

FUNCTION	The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome at a unique and precise dsDNA sequence to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer. Once the DNA is packaged, the terminase detaches from the portal and gets replaced by the tail to finish maturation of the virion.
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SUBUNIT	Homopentamer. Interacts with the terminase small subunit; the active complex is probably heterooligomeric. Interacts with the portal protein.
DOMAIN	The ATPase region is in the N-terminus, whereas the nuclease region is in the central part. The C-terminus is involved in prohead binding.
SIMILARITY	Belongs to the Teseptimavirus large terminase family.

Keywords [?]

Gene Ontology [?]

GO:0098009; Cellular component:viral terminase, large subunit

GO:0004519; Molecular function:endonuclease activity

GO:0046872; Molecular function:metal ion binding

GO:0004518; Molecular function:nuclease activity

GO:0051276; Biological process:chromosome organization

GO:0019073; Biological process:viral DNA genome packaging

Cross-references [?]

Features [?]

From: TERL_BPT7 (P03694)
Key	From	To	Description	Tag	Condition	FTGroup
REGION	1	229	/note="ATPase activity"
REGION	344	429	/note="Nuclease activity"
REGION	571	586	/note="Involved in prohead binding"
MOTIF	58	65	/note="Walker A motif"		[AG]-F-R-G-[AIV]-[AG]-K-[ST]
MOTIF	156	161	/note="Walker B motif"		[ILV]-[ILM]-[ILV]-[AF]-D-D
BINDING	364	364	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" /ligand_note="catalytic; for nuclease activity"		D	1
BINDING	364	364	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for nuclease activity"		D	1
BINDING	420	420	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" /ligand_note="catalytic; for nuclease activity"		E	1
BINDING	518	518	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" /ligand_note="catalytic; for nuclease activity"		D	1

Additional information [?]