HAMAP logo

HAMAP rule MF_04147

Send feedback

General rule information [?]

Accession MF_04147
Dates 7-FEB-2020 (Created)
19-NOV-2022 (Last updated, Version 6)
Name TERL_T7
Scope(s) Viruses
Template(s) P03694 (TERL_BPT7); P10310 (TERL_BPT3); [ Recover all ]
Triggered by HAMAP; MF_04147 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TERL
Protein name RecName: Full=Terminase, large subunit;
AltName: Full=DNA-packaging protein;
RecName: Full=ATPase;
RecName: Full=Endonuclease;

Comments [?]

FUNCTIONThe terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome at a unique and precise dsDNA sequence to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer. Once the DNA is packaged, the terminase detaches from the portal and gets replaced by the tail to finish maturation of the virion.
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SUBUNITHomopentamer. Interacts with the terminase small subunit; the active complex is probably heterooligomeric. Interacts with the portal protein.
DOMAINThe ATPase region is in the N-terminus, whereas the nuclease region is in the central part. The C-terminus is involved in prohead binding.
SIMILARITYBelongs to the Teseptimavirus large terminase family.

Keywords [?]

Gene Ontology [?]

GO:0098009; Cellular component:viral terminase, large subunit
GO:0004519; Molecular function:endonuclease activity
GO:0046872; Molecular function:metal ion binding
GO:0004518; Molecular function:nuclease activity
GO:0051276; Biological process:chromosome organization
GO:0019073; Biological process:viral DNA genome packaging

Cross-references [?]

Features [?]

From: TERL_BPT7 (P03694)
Key From To Description Tag Condition FTGroup
REGION 1 229 /note="ATPase activity"
REGION 344 429 /note="Nuclease activity"
REGION 571 586 /note="Involved in prohead binding"
MOTIF 58 65 /note="Walker A motif" [AG]-F-R-G-[AIV]-[AG]-K-[ST]
MOTIF 156 161 /note="Walker B motif" [ILV]-[ILM]-[ILV]-[AF]-D-D
BINDING 364 364 /ligand="Mg(2+)"
/ligand_note="catalytic; for nuclease activity"
D 1
BINDING 364 364 /ligand="Mg(2+)"
/ligand_note="catalytic; for nuclease activity"
D 1
BINDING 420 420 /ligand="Mg(2+)"
/ligand_note="catalytic; for nuclease activity"
E 1
BINDING 518 518 /ligand="Mg(2+)"
/ligand_note="catalytic; for nuclease activity"
D 1

Additional information [?]

Size range 500-680 amino acids
Related rules None
Fusion Nter: None Cter: None

View rule in raw text format (no links)